ExiProgen™

* Specifications of this product are subject to change without notice to improve product performance.

▶ Experimental data

1Protein expression and purification

• DNA concentration used: 6 - 10 μg (purity > 1.8)
• Protocol number : 902
• Total run time : < 6 h
• yield : <100 μg

Experimental Process

SDS-PAGE analysis
	<Simultaneous expression/purification of the same protein (GFP) 16 wells> Figure 1. GFP protein (His-tagged protein)'s expression/purification with ExiProgen™ M; AccuLadder™ Protein Size Maker (Low), E; expression sample P; purification sample
	<Simultaneous expression/purification of different kinds of proteins> Figure 2. His-tagged proteins expression/purification with ExiProgen™ M; AccuLadder™ Protein Size Maker (Low) E; expression sample, P; purification sample

2Total RNA Extraction From Tissue or cultured mammalian cell

• Sample: 15 ㎎ (rat liver tissue) or 1 X 10⁶ cells (HeLa)
• Protocol number: 202
• Average yield: 25 ㎍ (rat liver tissue), 10 ㎍ (HeLa)
• Average RQS: 9.5 over

※ RQS(RNA quality score): The quality of the extracted RNA is expressed as a numerical value, the height and width of each rRNA peak, and the peak of degraded RNA fragments are comprehensively calculated and displayed. It is the same concept as the RNA integrity number (RIN) developed by Agilent. It is usually expressed as a value from 0 to 10. The closer to 10, the better the quailty of RNA.

Experimental Process

• Analysis results of Total RNA extracted from tissues of Rat

Figure 3. Since each subunit of rRNA is well observed, it can be confirmed that RNA was intactly extracted without decomposition of RNA during total RNA extraction process.

• Results of Real-Time PCR using Total RNA extracted from tissues of Rat

Figure 4.Results of real time RT-PCR using AccuPower^® RocketScrpt ™ RT PreMix(K-2101, Bioneer), AccuPower^® DualStar™ qPCR PreMix(K-6110, Bioneer) and Exicycler™ 96 Real-Time Quantitative Thermal Block(A-2060)

• Analysis results of total RNA extracted from HeLa cell

Figure 5. Total RNA was extracted from HeLa cell (1 X 10⁶ cells) and analyzed for quality of extracted RNA using LabChip GX (Caliper Life Science). As each subunit of rRNA is well observed, intact RNA was extracted without RNA degradation during total RNA extraction. Also, the average yield was 10 ㎍ and the average RQS was 9.5 or more.

• Results of real-time PCR using total RNA extracted from HeLa cell

Figure 6. After the total RNA extraction from HeLa cell (1 X 10⁶ cells), it is results of real-time RT-PCR using AccuPower^® RocketScrpt ™ RT PreMix (K-2101, Bioneer), AccuPower^® DualStar ™ qPCR PreMix (K-6110, Bioneer) and Exicycler™ 96 Real-Time Quantitative Thermal Block (A-2060)

3Genomic DNA from cultured mammalian cell (HeLa)

• Sample volume: 1X10⁶ cells
• Protocol number: 107
• Total prep time: about 1hr 30min.
• Average yield: 4-8 μg

Experimental Process

• Agarose gel analysis

Figure 7. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X10⁶ cells of cultured HeLa cell and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH₂O as a negative control in DNA extraction. Note all samples have similar yields. Purity was also tested and was consistently between 1.8 and 2.0 (not shown).

4Genomic DNA from bacteria (E.coli)

• Sample volume: 1X10⁹ cells
• Protocol number: 109
• Total prep time: about 1hr 40min.
• Average yield: 8-12 μg

Experimental Process

• Agarose gel analysis

Figure 8. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X10⁹ cells of E.coli cells and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH₂O as a negative control in DNA extraction. Note all samples have similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

5Genomic DNA from tissue (bovine skeletal muscle)

• Sample volume: ~25 mg
• Protocol number: 102
• Total prep time: about 1hr 30min.
• Average yield: Up to 20 µg

Experimental Process

• Agarose gel analysis

Figure 9. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 25 mg of bovine tissues and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH₂O as a negative control in DNA extraction. Note all samples have similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

6Genomic DNA from plant tissue (spinach leaf)

• Sample volume: ~ 100 mg
• Protocol number: 104
• Total prep time: about 1 hr
• Average yield: Up to 5 µg

Experimental Process

• Agarose gel analysis

Figure 10. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 100 mg of spinach leaves and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH₂O as a negative control in DNA extraction. Note all samples have similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).