MagListo™ Protein G Kit

MagListo™ Protein G Kit allows rapid and easy purification of antibodies using magnetic separation method. It consists of Protein G Magnetic Nanobead and buffer for antibodies purification. Magnetic Nanobeads use silica beads containing magnetic particles and are coated on the beads with high purity (> 95%). These characteristics allow specific recognition of antibodies and purification of 1.2 mg Human IgG per 1 ml of bead solution. The recovered samples can be applied to antibody purification, immunoprecipitation, antigen-antibody interaction studies, protein complex studies, cell separation, etc.

$230.00

Cat. No.

K-7710

Product Overview

Features and Benefits

Reduced experimental time

Rapid with minimized loss through powerful magnetism of Magnetic Nanobeads
High binding capacity

Large surface area of Magnetic Nanobeads with average diameter of 400nm
Strong specificity
Reduced non-specific binding through the use of homogeneous spherical nanobeads

Application

• Antibody purification

• immunoprecipitation

• Antigen-Antibody interaction studies

• Protein complex studies

• cell separation

Technical/Specs

▶ Components

Components		Storage Temperature
AccuNanoBead ™ Protein G Magnetic NanoBeads	1 ml x 1 ea (4%)	2 - 8°C Storage
Binding & Washing Buffer	20 ml x 2 ea
Elution Buffer	1 ml x 2 ea
Neutralization Buffer	1 ml
Manual	1 ea

▶ Specifications

Feature	Specification
Matrix	Silica-coated Fe₃O₄
Concentration	40 mg/ml (4% suspension)
Max. Binding Capacity	> 30 μg Human IgG/mg beads sample
Storage and Stability	Beads can be stored at 2 - 8°C until the expiration date. Do not freeze and vortex vigorously.

▶ Binding affinity table

▶ Experiment Results

Figure 1. Comparison of protein binding efficiency of AccuNanoBead ™ Protein G Magnetic NanoBeads in MagListo™ Protein G Kit with a competitor.
A) Elution fraction contains Human IgG purified with Protein G magnetic bead from Bioneer(MagListo™), a competitor L or a competitor Q. Upper and lower bands are heavy (HC) and light chain (LC) of purified Human IgG in SDS-PAGE gel, respectively.
B) Comparison of binding capacity of Bioneer’s MagListo™ and competitor’s. Amount of bound IgG is quantified by UV-spectrophotometer. All reactions are carried out for 1 hour at room temperature. Human IgG is purchased from Sigma (Cat. no. I4506). M: protein marker (Bioneer, Cat. No. D-2020), Bioneer: MagListo™ Protein G Kit.

Figure 2. Purification of Human IgG with AccuNanoBead™ Protein G Magnetic NanoBeads in MagListo™ Protein G Kit.
IgG was purified from human whole blood. Arrows point to heavy and light chains of IgG, respectively.

- M: protein marker
- W: human whole blood
- U: unbound fraction
- E1 & 2: elution fraction 1 & 2.

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

▶ Ordering Information

Cat. No.	Product Description	Option
K-7710	MagListo™ Protein G Kit	40 mg/ml

▶ Related Products

Cat. No.	Product Description	Option
TA-1021-1	AccuNanoBead ™ Protein G Magnetic Nanobeads, size 400 nm	40 mg/1 ml, 1 ea
TA-1021-5		40 mg/1 ml, 5 ea
TA-1021-10		40 mg/1 ml, 10 ea
TM-1000	MagListo ™ Magnetic Separation Rack	1 ml x 8 holes
TM-1010		2 ml x 8 holes
TM-1020		15 ml x 6 holes
TM-1030		50 ml x 3 holes

Selection Guide

The BIONEER products for recombinant protein synthesis and purification are categorized as the following, Those can be selected according to the purpose.

To apply for the ExiProgen™ demo, plase contact sales@bioneer.co.kr .

Process

Product type

Equipment used

Service

DNA Template

Preparation