Features and Benefits
- Reduced experimental time
Rapid with minimized loss through powerful magnetism of Magnetic Nanobeads
- High binding capacity
Large surface area of Magnetic Nanobeads with average diameter of 400nm
- Strong specificity
Reduced non-specific binding through the use of homogeneous spherical nanobeads
Application
• Antibody purification
• immunoprecipitation
• Antigen-Antibody interaction studies
• Protein complex studies
• cell separation
▶ Components
Components |
Storage Temperature |
AccuNanoBead ™ Protein G Magnetic NanoBeads |
1 ml x 1 ea (4%) |
2 - 8°C Storage |
Binding & Washing Buffer |
20 ml x 2 ea |
Elution Buffer |
1 ml x 2 ea |
Neutralization Buffer |
1 ml |
Manual |
1 ea |
▶ Specifications
Feature |
Specification |
Matrix |
Silica-coated Fe3O4 |
Concentration |
40 mg/ml (4% suspension) |
Max. Binding Capacity |
> 30 μg Human IgG/mg beads sample |
Storage and Stability |
Beads can be stored at 2 - 8°C until the expiration date. Do not freeze and vortex vigorously. |
▶ Binding affinity table

▶ Experiment Results

Figure 1. Comparison of protein binding efficiency of AccuNanoBead ™ Protein G Magnetic NanoBeads in MagListo™ Protein G Kit with a competitor.
A) Elution fraction contains Human IgG purified with Protein G magnetic bead from Bioneer(MagListo™), a competitor L or a competitor Q. Upper and lower bands are heavy (HC) and light chain (LC) of purified Human IgG in SDS-PAGE gel, respectively.
B) Comparison of binding capacity of Bioneer’s MagListo™ and competitor’s. Amount of bound IgG is quantified by UV-spectrophotometer. All reactions are carried out for 1 hour at room temperature. Human IgG is purchased from Sigma (Cat. no. I4506). M: protein marker (Bioneer, Cat. No. D-2020), Bioneer: MagListo™ Protein G Kit.

Figure 2. Purification of Human IgG with AccuNanoBead™ Protein G Magnetic NanoBeads in MagListo™ Protein G Kit.
IgG was purified from human whole blood. Arrows point to heavy and light chains of IgG, respectively.
- M: protein marker
- W: human whole blood
- U: unbound fraction
- E1 & 2: elution fraction 1 & 2.
The BIONEER products for recombinant protein synthesis and purification are categorized as the following, Those can be selected according to the purpose.
To apply for the Exiprogen™ demo, plase contact sales@bioneer.co.kr .
Process |
Product type |
Equipment used |
Service |
DNA Template
Preparation
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Protein
Syn / Puri / Dialysis
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