Gene to Protein Synthesis Custom Order

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Services related to gene synthesis require some time to ship. Please order separately from the other products.
Only orders before 16:00 KST will start on the same day. (If we need to receive your samples, it will start on the date of their arrival.)
For genes exceeding 3kb, please fill out the Order Form and send it to geneorder@bioneer.co.kr.

Gene Synthesis Service [Order Notice]

Sequence Name

5' Add Sequence

3' Add Sequence

Sequence

Mutant Name

Mutant Sequence

Mutant Position [help]

Protein

Species

Library [help]

AccuGene Block Scale

End Type

Gene Synthesis Vector

Rapid Gene Synthesis Vector

Mutagenesis Vector

Codon Library Mutagenesis Vector

Gene to Protein Vector

Insert Type

Insert Size

Cloning Vector Size

Cloning Vector Name

Customer provided Vector

Cloning Vector Sequence

Product

Antibiotics Name

5' Enzyme Name

3' Enzyme Name

Forward Sequencing Primer Name [help]

Reverse Sequencing Primer Name [help]

Toxic

Gene Synthesis E-coli Stab culture Service

Gene Synthesis Plasmid Increase Service

Rapid Gene E-coli Stab culture Service

Rapid Gene Plasmid Increase Service

Mutant E-coli Stab culture Service

Mutant Plasmid Increase Service

Cloning E-coli Stab culture Service

Cloning Plasmid Increase Service

Codon Library E-coli Stab culture Service

Codon Library Plasmid Increase Service

His tag position

Disulfide bond

Storage buffer [help]

Gene synthesis product receipt [help]

Mutagenesis product receipt [help]

Codon Library Mutagenesis product receipt [help]

5' cap

3' poly(a) tail

Comments

Gene Synthesis Invoice Information

Qty

Product Overview

Overview

Gene to Protein Service is an integrated “gene synthesis-protein synthesis” service which can provide proteins and genes within at least 2 weeks at a reasonable price. Depending on the method of gene synthesis, it is classified as Standard and Cloning-free service with a wide variety of synthesizable proteins.

1. Standard Service
Standard Service synthesizes genes based on our Gene Synthesis Service. It is cloned into an expression vector for E. coli, followed by the protein synthesis service.

2. Cloning-free Service
Cloning-free Service synthesizes genes based on our AccuGeneBlock Service. PCR products are used to synthesize proteins. This service takes shorter time to complete than the other.

Features and Benefits

Advanced protein synthesis system

Synthesis of various proteins (enzymes, growth factors, hormones, antigen proteins, antibodies, etc.) possible, including toxic proteins that could not be done by traditional cell culture method.
Accuracy
100% guaranteed exact sequences using Automatic DNA sequencer (ABI 3730)
Codon optimization service
Complimentary codon optimization for custom gene to enhance protein expression and function.
Compatibility
Manufacture of target protein possible to be done by oneself if necessary, as this service utlizes Bioneer’s protein synthesis kits.

Workflow

▶ Comparsion of Service Time

▶ Schematics of Gene to Protein (Standard) Process

1Online quotation confirmation and order

Quote confirmation is available in online.

After confirming the quotation, proceed to add shopping cart and final order.

2Oligo synthesis

Proceed with design and synthesize oligo for gene synthesis.

3Gene Synthesis and Cloning

Proceed with gene synthesis.

4Screening

Proceed with colony screening.

5Sequencing

Proceed with sequencing.

6Data analysis

Proceed to Q.C based on sequencing data.

7Completed synthesis and delivery/ Protein service linkage

8Protein synthesis

Proceed with protein synthesis using ExiProgen™.

9Protein delivery

▶ Schematics of Gene to Protein (Cloning free) Process

1Online quotation confirmation and order

Quote confirmation is available in online.

After confirming the quotation, proceed to add shopping cart and final order.

2Oligo synthesis

Proceed with design and synthesize oligo for gene synthesis.

3Gene Synthesis

Proceed with gene synthesis.

4Data analysis

Proceed with Q.C process.

5Completed synthesis and delivery/ Protein service linkage

6Protein synthesis

Proceed with protein synthesis using ExiProgen™.

7Protein delivery

Literature/Support

◈ MSDS

• Gene to Protein Service MSDS

◈ Brochure

• All about Bioneer's Custom Services

◈ Quality Assurance

Bioneer is the holder of Quality Management Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

▶ Standard Gene to Protein Service

Cost of synthesis¹⁾		$1,600 (Normal sequence/300bp~1Kb)
Additional cost of gene synthesis²⁾		Gene synthesis - $0.45/bp (>1kb)
Period of synthesis		300~1500 bp	20~35 working days
		1501~3000 bp	30~40 working days
Delivery form	Gene	2 ~ 5 ug of lyophilized plasmid
	Protein³⁾	100 µg (0.1~1.0 mg/ml) of solution

1) Canceling your order within 5 days after receiving the confirmation email will charge 50% of the original price. After 5 days, 80% of the fee will be charged.
2) Additional costs may be charged upon requesting complicated sequences such as containing high or low GC content, repeat sequences or homopolymeric runs.
3) Failing protein synthesis will only cost $300 of set up charge. However, the gene synthesis fee will be charged normally. The synthesized gene will be sent along with the data report.

▶ Cloning-free Gene to Protein Service

Cost of synthesis		$1,400 (Normal sequences/300bp~1Kb)
Period of synthesis		10~15 working days
Delivery form	Gene	500 ng~1 µg of lyophilized plasmid PCR product
	Protein¹⁾	100 µg (0.1~1.0 mg/ml) of solution

1) Failing protein synthesis will only cost $300 of set up charge. However, the gene synthesis fee will be charged normally. The synthesized gene will be sent along with the data report.

▶ Contact us

• Contact
Please contact Tel. +82-42-930-8777(Customer Support Center) or +82-42-930-8793, 8515 (Gene Synthesis Team) or E-mail; geneorder@bioneer.co.kr

• Consultation time
Weekdays from 9:00 am to 6:00 pm (closed on weekends and holidays)

FAQ

How can I use the service?

All the services are available online.

Select the service and enter the required information. Add the product to the cart and proceed to payment for the order conformation.

How is Gene to Protein Service carried out?

After synthesizing the gene of the protein ordered, protein expression is done through ExiProgen™, along with purification.

The synthesized gene that expresses the target protein is added to the protein synthesis kit, mounted on an ExiProgen™ instrument, and run as described in the manual to obtain the purified protein easily.

What is the difference between Standard Gene to Protein Service and Cloning-free Gene to Protein Service?

Standard Gene to Protein Service synthesizes the target gene (300 bp~3000 bp). Then, it is cloned to expression vector (pBT7-N-His or pBT7-C-His) in the form of a plasmid. Afterwards, it is mounted in the protein synthesis kit for the target protein recovery.

Cloning-free Gene utilizes AccuGeneBlock service (300 bp~1000 bp). The gene is synthesized in the form of PCR product to be directly applied to the protein expression kit.

What is the composition of the buffer containing the protein sample? What if I want to change it?

We provide the basic protein dissolved in the following buffer.

Normal Proteins: 50 mM Tris-Cl (pH7.6), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.05% NaN3, 50% Glycerol

Disulfide Proteins: 50 mM Tris-Cl (pH7.6), 100 mM NaCl, 1 mM DTT, 0.05% NaN3, 50% Glycerol

If you want to receive your proteins in a specific buffer, please state its name in the order form. However, please note that additional time and costs will be required.

What kinds of kits are used for protein synthesis?

The kit used in the protein synthesis services depends on the protein characteristics as the following:

Protein type	Cat. No.	Product Description
Normal Protein	K-7300	ExiProgen™ EC Protein Synthesis Kit
Disulfide Protein	K-7330	ExiProgen™ EC-Disulfide Protein Synthesis Kit
His-tag free Protein	K-7320	ExiProgen™ EC-Tagfree Protein Synthesis Kit