ExiPrep™ Beef Genomic DNA Kit (96 reactions)

ExiPrep™ Beef Genomic DNA Kit allows extraction of genomic DNA for Korean beef discrimination using automatic nucleic acid extraction instruments: ExiPrep™16 series (ExiPrep™16, ExiPrep™16 Plus) and ExiProgen™. Pre-filled buffer cartridges with essential reagents for nucleic acid extraction can be used to isolate maximum of 16 samples simultaneously with ease. Furthermore, following the preparation method in the user manual, the Tissue Lysis buffer and Proteinase K included in this product can effectively break down animal tissues.

$363.00

Cat. No.

K-3200-CB

Product Overview

Features and Benefits

Pre-filled buffer cartidge system
Diluted enzymes and reagents essential for nucleic acid extraction
Great lysis optimization
Tissue Lysis Buffer and consumables (Disposable filter tip, Elution tube, etc.) in the kit for various animal tissue lysis
High-yield production of highly-pure nucleic acid
Implementation of Silica Magnetic Particles developed and produced by Bioneer's proprietary technology
High reproducibility
Reliable results through the use of automatic nucleic acid extraction instrument (ExiPrep™ 16, ExiPrep™ 16 Plus, ExiProgen™)

Application

• PCR

• Quantitative real-time PCR

• Gene cloning

• SNP analysis

• Genetics

Technical/Specs

▶ Specifications

Feature	Specification
Starting culture volume	10 - 40 mg
Elution volume	50 - 100 µl
Expected yield	up to 10 µg
Expected purity	A_260/280: > 1.8

▶ Components

Components	K-3200-CB
Buffer Cartridge ①	6 ea
Buffer Cartridge ②	6 ea
Beef Lysis Buffer	25 ml x 1 ea
Proteinase K powder, lyophilized	20 mg x 2 ea
Disposable Filter Tip	3 packs (32 ea/pack)
Reaction Tube (0.5 ml)	1 pack (96 ea/pack)
Elution Tube	8-tube strips x 12 ea
User Guide	1 ea

▶ Procedure

▶ Experimental data

Figure 1. Electrophoresis results of genomic DNA extracted from bovine tissues

Results of electrophoresis of genomic DNA extracted from bovine tissue samples (40 mg) on 1% agarose gel were shown above. To check the occurrence of cross-contamination during genomic DNA extraction, the bovine tissue samples were placed sparsely in a checkerboard pattern while the rest wells were filled with distilled water (D.W.). No signs of cross-contamination were found in the extracted genomic DNA, with the average yield of 10 µg and the mean purity (A_260/280) of more than or at least 1.8.

- M; Size marker
- S; Extraction with bovine tissue sample
- B; Extraction with D.W. only.

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

Cat. No.	Product Description	Option
K-3200-CB	ExiPrep™ Beef Genomic DNA Kit	96 reactions

* This kit can be used with ExiProgen™, ExiPrep™16 Plus, ExiPrep™16