MagListo™ Protein L Kit

MagListo™ Protein L Kit uses magnetic separation method that allows quick and easy purification of antibodies. It is consist of Protein L Magnetic Nanobead which consists of magnetic particles in silica beads, coated with high-purity (> 95%) of protein L. These characteristics allow specific recognition of antibodies which can purify 800 μg of Human IgG per 1 ml of bead solution. It is applied to antibody purification, immunoprecipitation, antigen-antibody interaction studies, protein complex studies, cell separation, etc

※This product is shipped with ice pack.

$230.00

Cat. No.

K-7730

Product Overview

Features and Benefits

Reduced experimental time
Rapid with minimized loss through powerful magnetism of Magnetic Nanobeads
High binding capacity
Large surface area of Magnetic Nanobeads with average diameter of 400nm
Strong specificity
Reduced non-specific binding through the use of homogeneous spherical nanobeads

Application

• Antibody purification

• Immunoprecipitation

• Antigen-Antibody interaction studies

• Protein complex studies

• Cell separation

Technical/Specs

▶ Components

Components		Storage Temperature
AccuNanoBead ™ Protein L Magnetic NanoBeads	1 ml x 1 ea (4%)	4 ~ 8°C Storage
Binding & Washing Buffer	20 ml x 2 ea
Elution Buffer	1 ml x 2 ea
Neutralization Buffer	1 ml
Manual	1 ea

▶ Specifications

Feature	Specification
Matrix	Silica-coated Fe₃O₄
Concentration	40 mg/ml (4% suspension)
Max. binding capacity	> 20 μg Human IgG/mg beads sample
Storage and stability	Beads can be stored at 2-8℃ until the expiration date. Do not freeze or vortex vigorously.

▶ Binding affinity table

▶ Experiment Results

Figure 1. Purification of Human IgG With MagListo™ Protein L Kit.

Elution fraction contains human IgG purified with protein L magnetic bead from Bioneer (MagListo™ ). Upper and lower bands are heavy (HC) and light chain (LC) of purified human IgG in SDS-PAGE gel, respectively.
- M: protein marker
- L: human plasma

Figure 2. Purification of scFv with MagListo™ Protein L Kit.
scFv was purified from cell lysate. Arrow points to scFv, about 25 kDa size.
- M: protein marker
- L: scFv expression cell lysate

Literature/Support

◈ Manuals

• MagListo™ Protein L Kit

• AccuNanoBead ™ Protein L Magnetic NanoBeads

◈ MSDS

• MSDS_MagListo™ Protein L Kit Magnetic Nanobeads

• MSDS_MagListo™ Protein L Kit Binding&Washing Buffer

• MSDS_MagListo™ Protein L Kit Elution Buffer

• MSDS_MagListo™ Protein L Kit Neutralization Buffer

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

Ordering Info

▶ Ordering Information

Cat. No.	Product Description	Option
K-7730	MagListo™ Protein L Kit	40 mg/ml

▶ Related Products

Cat. No.	Product Description	Option
TA-1023-1	AccuNanoBead ™ Protein L Magnetic NanoBeads, size 400nm	40 mg/1 ml, 1 ea
TA-1023-5		40 mg/1 ml, 5 ea
TA-1023-10		40 mg/1 ml, 10 ea

Selection Guide

The BIONEER products for recombinant protein synthesis and purification are categorized as the following, Those can be selected according to the purpose.

To apply for the ExiProgen™ demo, plase contact sales@bioneer.co.kr .

Process

Product type

Equipment used

Service

DNA Template

Preparation

Protein

Syn / Puri / Dialysis