Standard Protein Synthesis Service

After filling and sending the order form according [Quotation and order process] on the Ordering Information page, please send the template DNA samples according to the conditions below.

¹⁾ Additional cost of $100 will be charged if the sample is in the form of non-expression vector as these require process of template DNA preparation for protein expression

²⁾ DNA size represents the size of the ORF (Open Reading Frame) except the vector.

You can use DNA amplication service. but an additional cost of $50/10 ug DNA will be charged. 

Generally, the protein synthesis takes one day, but depending on the amount of requested protein, it may take a longer time.

The exact time can be checked through the quotation.

Our company has synthesis data for molecular weights of 10~150 kDa.

If your requested protein is out of range, a fee of $100 will be charged as additional experiments will be required.

If you don’t have the template DNA for your desired proteins, you may try using Gene to Protein service which not only synthesizes genes, but also provide protein synthesis service.

In this case, you must pay an additional cost for the gene expression.

Please refer to Gene to Protein Service order page for more details.

Generally, the buffer composition for the protein delivery is as the following:

Normal Proteins: 50 mM Tris-Cl (pH7.6), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.05% NaN3, 50% Glycerol

Disulfide Proteins: 50 mM Tris-Cl (pH7.6), 100 mM NaCl, 1 mM DTT, 0.05% NaN3, 50% Glycerol

If you want to recieve your proteins in a specific buffer, please state its name in the order form. However, please note that additional time and costs will be required.

The kits used in the protein synthesis services depends on the protein characteristics Refer to the following table:

In case of E. coli expression vector having both T7 promoter and His-tag, protein synthesis can be done immediately without other steps.

On the other hand, in case of non-expression vector, there is no sequence that can undergo protein expression or purification. Thus, additional procedures must be done beforehand.

A two-step process is done after the primer design. First, a primer design for the gene amplification is performed and PCR is performed for procedure of making template DNA for protein expression. Then, a protein synthesis is done with the purified template DNA.