After filling and sending the order form according [Quotation and order process] on the Ordering Information page, please send the template DNA samples according to the conditions below.
|DNA type||E. coli expression vector(ex) pET etc T7-based vetor)||Non expression vector1) (ex) Cloning vector, PCR product)|
|DNA size2)||0.3 kb ~ 4.0 kb||0.3 kb ~ 1.5 kb|
|DNA amount||10 ug or more (A260/280 : > 1.7, A260/230 : >1.5)||2 ug or more (A260/280 : > 1.7, A260/230 : >1.5)|
1) Additional cost of $100 will be charged if the sample is in the form of non-expression vector as these require process of template DNA preparation for protein expression
2) DNA size represents the size of the ORF (Open Reading Frame) except the vector.