ExiPrep™ Tissue Genomic DNA Kit (96 reactions)

ExiPrep™ Tissue Genomic DNA Kit allows rapid extraction of highly pure genomic DNA with high yield from animal tissue samples using automatic nucleic acid extraction instruments: ExiPrep™16 series (ExiPrep™16, ExiPrep™16 Plus) and ExiProgen™. Pre-filled buffer cartridges with essential reagents for nucleic acid extraction can be used to isolate maximum of 16 samples simultaneously with ease. Furthermore, following the preparation method in the user manual, the Tissue Lysis Buffer and Proteinase K included in this product can effectively break down animal tissues.

Application Note

• ExiPrep™ Tissue Genomic DNA Kit_Animal tissue(mouse).pdf

$363.00

Cat. No.

K-3225

Product Overview

Features and Benefits

Pre-filled buffer cartidge system
Diluted enzymes and reagents essential for nucleic acid extraction
Great lysis optimization
Tissue Lysis Buffer and consumables (Disposable filter tip, Elution tube, etc.) included in the kit for various animal tissue lysis
Quick purification time
Simultaneous processing of at least 1 to 16 large samples at maximum
High-quality, high-yield quick productivity
Implementation of Silica Magnetic Particles developed and produced by Bioneer's proprietary technology
High reproducibility
Reliable results through the use of automatic nucleic acid extraction instrument (ExiPrep™ 16, ExiPrep™ 16 Plus, ExiProgen™)

Application

• PCR

• Quantitative real-time PCR

• Gene cloning

• SNP analysis

• Genetics

Technical/Specs

▶ Specifications

Feature	Specification
Starting culture volume	up to 25 mg
Elution volume	50 - 100 µl
Expected yield	5 - 20 µg
Expected purity	A_260/280: > 1.8

▶ Components

Components	Quantity	Note
Buffer Cartridge ①	6 ea
Buffer Cartridge ②	6 ea
Tissue lysis buffer	1 ea	25 ml
Disposable filter tip	3 pack	32 ea/pack
Reaction tube	1 pack	96 ea/pack
Elution tube	1 pack	12 ea/pack
User's Guide	1 ea

▶ Procedure

Tissue_Genomic_DNA_Kit

▶ Experimental data

Figure 1. Agarose gel electrophoresis results of genomic DNA extracted from mouse tissue

accupower

Results of electrophoresis of genomic DNA extracted from mouse tissue cells (lung, 25 mg) on 1% agarose gel were shown above. To check the occurrence of cross-contamination during genomic DNA extraction, mouse tissue samples were placed sparsely in a checkerboard pattern while the rest wells were filled with distilled water (D.W.). No signs of cross-contamination were found in the extracted genomic DNA, with the average yield of 20 µg and the mean purity (A_260/280)of at least 1.8.

- M; Size marker
- S; Extraction with mouse tissue sample
- B; Extraction with D.W. only.

accupower

Results of electrophoresis of genomic DNA extracted from a mouse tail tip (~ 1 cm) on 1% agarose gel were shown above. To check the occurrence of cross-contamination during genomic DNA extraction, mouse tail-tip samples were placed sparsely in a checkerboard pattern while the rest wells were filled with distilled water (D.W.). No signs of cross-contamination were found in the extracted genomic DNA, with the average yield of 20 µg and the mean purity (A_260/280) of at least 1.8.

- M; Size marker
- S; Extraction with mouse tail-tip sample
- B; Extraction with D.W. only.

Literature/Support