Features and Benefits
-
Using pre-filled buffer cartridge system with diluted enzyme and reagent required for nucleic acid extraction
-
Provide Resuspension Buffer and consumables (disposable filter tip, elution tube, etc.) optimized for re-suspension and lysis of Bacterial cell
-
Use of silica magnetic particles developed and produced by Bioneer's proprietary technology, and extraction of high purity nucleic acid with high yield
-
It is a product using automatic nucleic acid extraction instrument (ExiPrep™16 Plus, ExiProgen™) and produces reproducible results.
Application
• PCR
• Quantitative real-time PCR
• Gene cloning
• Genetics
• Classification of bacteria
▶ Specifications
| Feature |
Specification |
| Starting culture volume |
up to 1X109 cells |
| Elution volume |
50 - 100 µl |
| Expected yield |
5-15 µg |
| Expected purity |
A260/280 > 1.8 |
▶ Components
| Components |
Quantity |
Note |
| Buffer Cartridge ① |
6 ea |
|
| Buffer Cartridge ② |
6 ea |
|
| Resuspension buffer |
1 ea |
25 ml |
| Disposable filter tip |
3 pack |
32 ea/pack |
| Elution tube |
1 pack |
12 ea/pack |
| Manual |
1 ea |
|
▶ Procedure
▶ Experimental data
Figure 1. Results of experiment using E.coli cell (1X109 cells)
Results of 1% agarose gel electrophoresis of genomic DNA extracted from E.coli cell (1X109 cells). To observe cross-contamination that may occur during the extraction process, the E.coli cell samples were placed in a checkerboard pattern, and the rest of the wells were filled with D.W. And genomic DNA extraction was performed. As a results, No cross-contamination could be detected. The yields of extracted genomic DNA were found to be an average of 10 µg and the purity (A260/280) averaged 1.8 or more.
- M; Size marker
- S; Extraction with E.coli cell sample
- B; Extraction with D.W. only.
Figure 2. Results of STD testing using urine sample
Results of real-time PCR of bacterial genomic DNA from 10 ml urine sample using AccuPower® CT & NG Real-time PCR Kit (CNM-1111, Bioneer), Exicycler™ 96 Real-Time Quantitative Thermal Block (A-2060).
- Blue; IPC (Internal Positive Control)
- Black; CT positive result (CT positive sample)
- Pink; NG positive result (NG positive sample)
