ExiProgen™ is an instrument that automatically synthesizes protein from DNA. This product performs In-vitro transcription and translation automatically when loaded with DNA coding a protein and a protein synthesis kit. By purifying this protein through affinity chromatography method, more than 90% pure protein can be obtained within 6 hours and up to 16 species can be produced at one time. In addition, highly expressed affinity tagged proteins can be purified in cell lines with high purity.
Application Note
• ExiProgen™ Application_Fluorescent proteins
• ExiProgen™ Application_High molecular weight protein
• ExiProgen™ Application_in vivo protein expression pET vectors
Protein synthesis using ExiProgen™ is carried out using its compatible ExiProgen™ Protein Synthesis Kit Series. ExiProgen™ performs protein expression using Cell-free Protein Synthesis system, and affinity purification using Ni-NTA magnetic beads, both in a fully-automated way. After adding a target gene to the template DNA sample and running the instrument, the protein is expressed in 3 hours with the help of Cell-free Protein Expression reagent. The expressed protein is then collected using Ni-NTA magnetic bead, and is purified using a reagent to yield a final high-purity target proteins.
ExiProgen™ DNA/RNA Kit consists of reagents and silica magnetic beads optimized for efficient nucleic acid extraction from various samples. The nucleic acid eluted from the sample is collected by a silica magnetic bead, washed with a washing solution and extracted with a high purity nucleic acid.
Simplified usage and reproducible results with built-in nucleic acid extraction and protein purification protocols optimized for various samples (whole blood, tissue, cell, bacteria, plant, etc.)
Minimized protein denaturation by installing cooling reaction block inside the instrument keeping the temperature of elution tube rack below 10°C
Quick performance of nucleic acid extraction and protein purification with great purity and efficiency by attaching magnetic particles to the bottom of each well equally and rapidly by magnetic blocks attached to the instrument
Increased elution efficiency allowing complete evaporation of alcohols by its heating block (Korean Patent No. 10-1025135)
Substantial blocking of cross-contamination, which may occur in other wells by the aerosols generated from the tip, through the sophisticated designs drop-contamination prevention device located at the bottom of the filter tip
Sterilization to prevent infection for the users by the samples through the UV lamp installed inside the instrument
User-friendly UI capable of checking the experimental progress rate real-time without the need of seperate PC by equipping with a 13.3-inch touch screen on the front of the device
Minimized error range, that may occur during the automatic dispensing of the solution, through installment of an adjustable syringe motor up to 0.1 mm capable of stably distributing in a volume of 25 ㎕ ~ 1000 ㎕ for application to various protocols and obtaining of experimental results
