ExiProgen™

ExiProgen™ is an instrument that automatically synthesizes protein from DNA. This product performs In-vitro transcription and translation automatically when loaded with DNA coding a protein and a protein synthesis kit. By purifying this protein through affinity chromatography method, more than 90% pure protein can be obtained within 6 hours and up to 16 species can be produced at one time. In addition, highly expressed affinity tagged proteins can be purified in cell lines with high purity.                                                                                                 

 Application Note

• ExiProgen™ Application_Fluorescent proteins

• ExiProgen™ Application_High molecular weight protein

• ExiProgen™ Application_in vivo protein expression pET vectors

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SKU
A-5041

Overview

Principle of protein synthesis and purification

ExiProgen™ Protein Synthesis Kit contains a Ni-NTA magnetic bead and two types of reagents: one optimized for efficient synthesis of various proteins and the other for purification of synthesized proteins. The target proteins synthesized from recombinant plasmid DNA are collected by Ni-NTA magnetic beads, then washed with washing solution and isolated with high-purity.

Nucleic Acid Extraction Principle

ExiProgen™ DNA/RNA Kit consists of reagents and silica magnetic beads optimized for efficient nucleic acid extraction from various samples. The nucleic acid eluted from the sample is collected by a silica magnetic bead, washed with a washing solution and extracted with a high purity nucleic acid.

Product Function

  • Fast protein production
    Rapid synthesis of proteins from genes in about 6 hours

  • DNA/RNA Purification
    Genomic DNA auto-purification from various biological samples such as animal, plant blood or tissue

  • Affinity protein purification
    Automatic purification of protein through affinity tagging

Features and Benefits

  • Built-in optimized nucleic acid and protein synthesis protocol

    Simplified usage and reproducible results with built-in nucleic acid extraction and protein purification protocols optimized for various samples (whole blood, tissue, cell, bacteria, plant, etc.)

  • High stability

    Minimized protein denaturation by installing cooling reaction block inside the instrument keeping the temperature of elution tube rack below 10°C

  • High-pure nucleic acid extraction & protein purification

    Quick performance of nucleic acid extraction and protein purification with great purity and efficiency by attaching magnetic particles to the bottom of each well equally and rapidly by magnetic blocks attached to the instrument
    Increased elution efficiency allowing complete evaporation of alcohols by its heating block (Korean Patent No. 10-1025135)

  • Contamination prevention device

    Substantial blocking of cross-contamination, which may occur in other wells by the aerosols generated from the tip, through the sophisticated designs drop-contamination prevention device located at the bottom of the filter tip

  • Automatic UV sterilization Function

    Sterilization to prevent infection for the users by the samples through the UV lamp installed inside the instrument

  • Easy-to-use

    User-friendly UI capable of checking the experimental progress rate real-time without the need of seperate PC by equipping with a 13.3-inch touch screen on the front of the device

  • Excellent precision

    Minimized error range, that may occur during the automatic dispensing of the solution, through installment of an adjustable syringe motor up to 0.1 mm capable of stably distributing in a volume of 25 ㎕ ~ 1000 ㎕ for application to various protocols and obtaining of experimental results

Application

• Enzyme engineering

• Protein evolution

• Synthetic biology

• Bio-energy R&D

• Protein drug R&D

Experimental Process

Specifications

Dimensions (cm) 32 (W) x 53.5 (D) x 50 (H)
Weight (kg) 27
Temperature range 15-35℃
Humidity range 20-80%, no condensation
Operating system Standalone
User interface 320 x 240 touch screen graphic LCD
Input voltage 100 – 240 VAC
Frequency 50 / 60 Hz
UV sterilization 15 minute cycle
Communications TCP/IP
Heat block 40-90℃

Function

DNA/RNA Extraction O
Protein Expression O
Protein Purification O
TFT Touch Screen O
Sample Number 16 samples
UV sterilization O
Heating & magnetic block O
Cartridge Contamination shield O
TCP/IP network connection Numerous equipment can be connected
Cooling system for sample store O
Automatic dispensing O
Power supply 24 VDC, 7.5 A(180 W)

* Specifications of this product are subject to change without notice to improve product performance.

Experimental data

1Protein expression and purification

  • DNA concentration used: 6 - 10 μg (purity > 1.8)
  • Protocol number : 902
  • Total run time : < 6 h
  • yield : <100 μg

Experimental Process

ExiProgen
SDS-PAGE analysis
ExiProgen

<Simultaneous expression/purification of the same protein (GFP) 16 wells>
Figure 1. GFP protein (His-tagged protein)'s expression/purification with ExiProgen
M; AccuLadder™ Protein Size Maker (Low),
E; expression sample
P; purification sample
ExiProgen <Simultaneous expression/purification of different kinds of proteins>
Figure 2. His-tagged proteins expression/purification with ExiProgen
M; AccuLadder™ Protein Size Maker (Low)
E; expression sample,
P; purification sample


2Total RNA Extraction From Tissue or cultured mammalian cell

  • Sample: 15 ㎎ (rat liver tissue) or 1 X 106 cells (HeLa)
  • Protocol number: 202
  • Average yield: 25 ㎍ (rat liver tissue), 10 ㎍ (HeLa)
  • Average RQS: 9.5 over

RQS(RNA quality score): The quality of the extracted RNA is expressed as a numerical value, the height and width of each rRNA peak, and the peak of degraded RNA fragments are comprehensively calculated and displayed. It is the same concept as the RNA integrity number (RIN) developed by Agilent. It is usually expressed as a value from 0 to 10. The closer to 10, the better the quailty of RNA.

Experimental Process

ExiProgen

Analysis results of Total RNA extracted from tissues of Rat


ExiProgen

Figure 3. Since each subunit of rRNA is well observed, it can be confirmed that RNA was intactly extracted without decomposition of RNA during total RNA extraction process.



Results of Real-Time PCR using Total RNA extracted from tissues of Rat


ExiProgen

Figure 4.Results of real time RT-PCR using AccuPower® RocketScrpt ™ RT PreMix(K-2101, Bioneer), AccuPower® DualStar™ qPCR PreMix(K-6110, Bioneer) and Exicycler™ 96 Real-Time Quantitative Thermal Block(A-2060)



Analysis results of total RNA extracted from HeLa cell


ExiProgen

Figure 5. Total RNA was extracted from HeLa cell (1 X 106 cells) and analyzed for quality of extracted RNA using LabChip GX (Caliper Life Science). As each subunit of rRNA is well observed, intact RNA was extracted without RNA degradation during total RNA extraction. Also, the average yield was 10 ㎍ and the average RQS was 9.5 or more.



Results of real-time PCR using total RNA extracted from HeLa cell


ExiProgen

Figure 6. After the total RNA extraction from HeLa cell (1 X 106 cells), it is results of real-time RT-PCR using AccuPower® RocketScrpt ™ RT PreMix (K-2101, Bioneer), AccuPower® DualStar ™ qPCR PreMix (K-6110, Bioneer) and Exicycler™ 96 Real-Time Quantitative Thermal Block (A-2060)



3Genomic DNA from cultured mammalian cell (HeLa)

  • Sample volume: 1X106 cells
  • Protocol number: 107
  • Total prep time: about 1hr 30min.
  • Average yield: 4-8 μg

Experimental Process

ExiProgen

Agarose gel analysis

ExiProgen

Figure 7. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X106 cells of cultured HeLa cell and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have similar yields. Purity was also tested and was consistently between 1.8 and 2.0 (not shown).



4Genomic DNA from bacteria (E.coli)

  • Sample volume: 1X109 cells
  • Protocol number: 109
  • Total prep time: about 1hr 40min.
  • Average yield: 8-12 μg

Experimental Process

ExiProgen

Agarose gel analysis

ExiProgen

Figure 8. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X109 cells of E.coli cells and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).



5Genomic DNA from tissue (bovine skeletal muscle)

  • Sample volume: ~25 mg
  • Protocol number: 102
  • Total prep time: about 1hr 30min.
  • Average yield: Up to 20 µg

Experimental Process

ExiProgen

Agarose gel analysis

ExiProgen

Figure 9. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 25 mg of bovine tissues and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).



6Genomic DNA from plant tissue (spinach leaf)

  • Sample volume: ~ 100 mg
  • Protocol number: 104
  • Total prep time: about 1 hr
  • Average yield: Up to 5 µg

Experimental Process

ExiProgen

Agarose gel analysis

ExiProgen

Figure 10. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 100 mg of spinach leaves and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).

Manual

Manual_ExiProgen


Brochure

ExiProgen

Guide book_ExiProgen


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate

ISO 13485 - certificate


Technical support

Email: Proteinsupport@bioneer.co.kr


Cat. No. Product Description Price
A-5041 ExiProgen Inquire

Related Products

1Protein Synthesis/Purification Kits for ExiProgen™)

Cat. No. Product Description
K-7220 ExiProgen™ His-tagged Protein Purification Kit, 16 reactions
K-7221 ExiProgen™ His-tagged Protein Purification kit, 32 reactions
K-7300 ExiProgen™ EC Protein Synthesis Kit, 16 reactions
K-7301 ExiProgen™ EC Protein Synthesis Kit, 32 reactions
K-7302 ExiProgen™ EC Protein Synthesis Kit, 96 reactions
K-7310 ExiProgen™ EC-Maxi Protein Synthesis Kit, 8 reactions
K-7320 ExiProgen™ EC-Tagfree Protein Synthesis Kit, 8 rxns
K-7330 ExiProgen™ EC-Disulfide Protein Synthesis Kit, 8 reactions


2Nucleic Acid Extraction Kits for ExiProgen™)

Cat. No. Product Description
K-3200-CB ExiPrep™ Beef Genomic DNA Kit
K-3200-CR ExiPrep™ Rice Genomic DNA Kit
K-4212 ExiPrep™ Plus Tissue Genomic DNA Kit, 96 reactions
K-4214 ExiPrep™ Plus Bacteria Genomic DNA Kit, 96 reactions
K-4215 ExiPrep™ Plus Plant Genomic DNA Kit, 96 reactions
K-4244 ExiPrep™ Plus Plant total RNA Kit, 96 reactions
K-4271 ExiPrep™ Plus Viral DNA/ RNA Kit, 96 reactions
K-4272 ExiPrep™ Plus Viral DNA Kit, 96 reactions
K-4273 ExiPrep™ Plus Viral RNA Kit, 96 reactions


3Accessories

Cat. No. Product Description
A-5041-A Accessories Set for ExiProgen™ (A2,3,4,5,6,7,10)
A-5041-A1 Multi Puncher (option)
A-5041-A2 Setup Tray
A-5041-A3 Disposable Tip Rack
A-5041-A4 Elution Tube Rack
A-5041-A5 Reaction Block (For Protein synthesis)
A-5041-A6 Waste Tray
A-5041-A7 Hole Puncher (6-hole)
A-5041-A9 AC Adapter for ExiProgen™, ExiPrep™16 Dx
A-5041-A10 Contamination Shield

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