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ExiPrep™ Plus Seed Genomic DNA Kit (96 reactions)
ExiPrep™ Plus Seed Genomic DNA Kit allows rapid extraction of highly-pure genomic DNA with high-yield from various plant samples such as seed, tissue, root, etc, using automatic nucleic acid extraction instruments: ExiPrep™16 Plus and ExiProgen™. Pre-filled buffer cartridges with essential reagents for nucleic acid extraction can be used to isolate maximum of 16 samples simultaneously with ease. Furthermore, Seed Lysis Buffer and Proteinase K included this product can break down plant tissues while Precipitation Buffer can effectively remove impurities.
Features and Benefits
-
Pre-filled buffer cartidge system
Diluted enzymes and reagents essential for nucleic acid extraction -
Great lysis optimization
Plant Lysis Buffer and consumables (Disposable filter tip, Elution tube, etc.) in the kit for various plant samples -
High-yield production of highly-pure nucleic acid
Implementation of Silica Magnetic Particles developed and produced by Bioneer's proprietary technology -
High reproducibility
Reliable results through the use of automatic nucleic acid extraction instrument (ExiPrep™ 16 Plus and ExiProgen™)
Application
• PCR
• Quantitative real-time PCR
• Gene cloning
• Genetics
• GMO detection
▶ Specifications
Feature | Specification |
Starting material | up to 50 mg (seed), up to 100 mg (leaf) |
Elution volume | 50-100 μl |
Expected yield | 0.25-5 μg |
Expected purity | A260/280: > 1.7 |
▶ Components
Components | Quantity | Note |
Buffer Cartridge ① | 6 ea | |
Buffer Cartridge ② | 6 ea | |
Seed Lysis Buffer | 1 ea | 30 ml |
Precipitation Buffer | 1 ea | 10 ml |
Proteinase K | 2 ea | 20 mg/tube |
RNase A | 2 ea | 24 mg/tube |
Disposable filter tip | 3 pack | 32 ea/pack |
Elution tube | 1 pack | 12 ea/pack |
User's Guide | 1 ea |
▶ Experimental data
Figure 1. Comparison of genomic DNA extracted from Citrullus lanatus seed (50 mg) using ExiPrep™ Plus Seed Genomic DNA Kit (Bioneer, automatic), ExiPrep™ Plus Plant Genomic DNA Kit (Bioneer, automatic), those done using MagListo™ 5M Plant Genomic DNA Extraction Kit (Bioneer, manual/magnetic bead) and those done using a competitor’s kit (Competitor Q, manual/single column).
M: Bioneer 1 kb ladder
1-2: Bioneer ExiPrep™ Plus Seed Genomic DNA Kit, mean yield; 2.8 μg, mean purity; 1.74
3-4: Bioneer ExiPrep™ Plus Plant Genomic DNA Kit, mean yield; 1.9 μg, mean purity; 1.4
5-6: Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit, mean yield; 3.4 μg, mean purity; 1.66
7-8: Competitor Q Genomic DNA Extraction Kit, mean yield; 2.1 μg, mean purity; 1.5
Figure 2. Comparison of genomic DNA extracted from Zea mays seed (50 mg) using ExiPrep™ Plus Seed Genomic DNA Kit (Bioneer, automatic), ExiPrep™ Plus Plant Genomic DNA Kit (Bioneer, automatic), that done using MagListo™ 5M Plant Genomic DNA Extraction Kit (Bioneer, manual/magnetic bead) and that done using a competitor’s kit (Competitor Q, manual/single column).
M: Bioneer 1 kb ladder
1-2: Bioneer ExiPrep ™ Plus Seed Genomic DNA Kit, mean yield; 3.7 μg, mean purity; 1.70
3-4: Bioneer ExiPrep™ Plus Plant Genomic DNA Kit, mean yield; 1.6 μg, mean purity; 2.1
5-6: Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit, mean yield; 2.7 μg, mean purity; 1.67
7-8: Competitor Q Genomic DNA Extraction Kit, mean yield; 0.9 μg, mean purity; 1.91
Figure 3. Comparison of genomic DNA extracted from Callistephus chinensis seed (50 mg) using ExiPrep™ Plus Seed Genomic DNA Kit (Bioneer, automatic), those done using ExiPrep™ Plus Plant Genomic DNA Kit (Bioneer, automatic), and that done using MagListo™ 5M Plant Genomic DNA Extraction Kit (Bioneer, manual/magnetic bead).
M: Bioneer 1 kb ladder
1-2: Bioneer ExiPrep™ Plus Seed Genomic DNA Kit, mean yield; 4.4 μg, mean purity; 1.58
3-4: Bioneer ExiPrep™ Plus Plant Genomic DNA Kit, mean yield; 4.7 μg, mean purity; 1.66
5-6: Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit, mean yield; 5.2 μg, mean purity; 1.61
Figure 4. Comparison of Conventional PCR targeting sus1 (sucrose synthase 1) gene from Maize genomic DNA purified with ExiPrep™ Plus Seed Genomic DNA Kit (Bioneer, automatic), that using MagListo™ 5M Plant Genomic DNA Extraction Kit (Bioneer, manual/magnetic bead) and that using a competitor’s kit (Competitor Q, single column).
M: Bioneer 100bp plus ladder
1-2: PCR product from genomic DNA of 1 mg seed purified with ExiPrep™ Plus Seed Genomic DNA Kit
3-4: PCR product from genomic DNA of 5 mg seed purified with ExiPrep™ Plus Seed Genomic DNA Kit
5-6: PCR product from genomic DNA of 10 mg seed purified with ExiPrep™ Plus Seed Genomic DNA Kit
7-8: PCR product from genomic DNA of 50 mg seed purified with ExiPrep™ Plus Seed Genomic DNA Kit
9-10: PCR product from genomic DNA of 50 mg seed purified with MagListo™ 5M Plant Genomic DNA Extraction Kit
11-12: PCR product from genomic DNA of 50 mg seed purified with competitor Q kit
13-14: Negative control
◈ Manual
• ExiPrep™ Plus Seed Genomic DNA Extraction Kit
◈ MSDS
• ExiPrep™ Resuspension/Lysis Buffer
• ExiPrep™ Precipitation Buffer
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
Cat. No. | Product Description | Option |
K-4217 | ExiPrep™ Plus Seed Genomic DNA Kit | 96 reactions |