SARS-CoV-2 Mutation Research
COVID-19 virus are constantly evolving to new variants which have different shedding capacity and susceptibility to vaccine, which is mainly caused by the change of spike protein. To prevent the super-spreading of the variants, the S protein should be sequenced more quickly and efficiently.
AccuPower® SARS-CoV-2 S gene Amplification Kit provides the more fast and convenient solution for sequencing of S gene. The kit contains every RT-PCR components including primers and enzymes. Just add your RNA sample into 4 tubes of kit, you can amplify whole S gene for direct sanger sequencing.
You can also purchase an additional PCR kit to analyze the S-Protein gene sequence of fewer positive samples, such as 100 copies or less.
Direct Sanger Sequencing is possible after PCR purification without cloning.
Step 1 Sample Loading |
Step 2 Viral RNA Extraction |
Step3 Amplification & Diagnosis |
Step 4 S-gene Amplification |
Step 5 PCR Clean-up |
Step 6 Sanger Sequencing |
Step 7 Variant Analysis |
Step 1: Sample Loading | |||||||||||||||||||||||||||||||
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Step 2: Viral RNA Extraction | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Spin column type | Magnetic Bead type | Automatic type | ||||||||||||||||||||||||||||||||||||||||||||||||||||
![]() AccuPrep® Viral RNA Extraction Kit |
![]() MagListo™ 5M Viral DNA/RNA Extraction Kit |
![]() ExiPrep™96 Lite |
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Step 3: Amplification & Diagnosis | |||||||||||||||||||||||||||||||
Diagnostics kit | Real-Time PCR | ||||||||||||||||||||||||||||||
![]() AccuPower® SARS-CoV-2 Multiplex Real-Time RT-PCR Kit |
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Step 4: S gene Amplification | |||||||||||||||||||||||||||||||
AccuPower® SARS-CoV-2 S-gene Amplification Kit (K-2520) | AccuPower® SARS-CoV-2 S-gene Amplification Additional Kit for low copy sample (K-2521) |
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Kit Components | |||||||||||||||||||||||||||||||
① 1st S gene Full length RT-PCR (96 tests X 1 plate) ② S gene Fragment RT-PCR (24 tests X 4 plates) |
Additional Kit for low copy sample (24 tests X 1 plate) | ||||||||||||||||||||||||||||||
Workflow | |||||||||||||||||||||||||||||||
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Step 5: PCR Clean-up | |||||||||||||||||||||||||||||||
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Step 6: Sanger Sequencing Service | |||||||||||||||||||||||||||||||
Fast and cost-effective Bioneer's Sanger sequencing service | |||||||||||||||||||||||||||||||
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* The primer is free if you use the Sanger sequencing service in Bioneer. |