SARS-CoV-2 Mutation Research

COVID-19 virus are constantly evolving to new variants which have different shedding capacity and susceptibility to vaccine, which is mainly caused by the change of spike protein. To prevent the super-spreading of the variants, the S protein should be sequenced more quickly and efficiently. 
AccuPower® SARS-CoV-2 S gene Amplification Kit provides the more fast and convenient solution for sequencing of S gene. The kit contains every RT-PCR components including primers and enzymes. Just add your RNA sample into 4 tubes of kit, you can amplify whole S gene for direct sanger sequencing.
You can also purchase an additional PCR kit to analyze the S-Protein gene sequence of fewer positive samples, such as 100 copies or less.
Direct Sanger Sequencing is possible after PCR purification without cloning.

Step 1
Sample
Loading
Step 2
Viral RNA
Extraction
Step3
Amplification
& Diagnosis
Step 4
S-gene
Amplification
Step 5
PCR
Clean-up
Step 6
Sanger
Sequencing
Step 7
Variant
Analysis

Step 1: Sample Loading
  • Collection samples from Patients
  • Specimen
    - Nasopharyngeal & Oropharyngeal swab,
     Sputum
  • Loading into RNA extraction Catridge

Step 2: Viral RNA Extraction
Spin column type Magnetic Bead type Automatic type

AccuPrep® Viral RNA Extraction Kit

MagListo™ 5M Viral DNA/RNA Extraction Kit

ExiPrep™96 Lite
Cat. No. Product Description Specification
K-3033 AccuPrep® Viral RNA Extraction Kit 100 reactions
K-3624 MagListo™ 5M Viral DNA/RNA Extraction Kit 100 reactions
TM-1011 MagListo™ 2-12h Magnetic Separation Rack 2 ml x 12 holes
A-5250 ExiPrep™96 Lite -

Step 3: Amplification & Diagnosis
Diagnostics kit Real-Time PCR 

AccuPower® SARS-CoV-2 Multiplex Real-Time RT-PCR Kit


Exicycler 96/384 Real-Time Quantitative Thermal Block

Cat. No. Product Description
A-2060-1 Exicycler™ 96 Real-Time Quantitative Thermal Block
A-2061 Exicycler™ 384 Real-Time Quantitative Thermal Block

Step 4: S gene Amplification
AccuPower® SARS-CoV-2 S-gene Amplification Kit (K-2520) AccuPower® SARS-CoV-2 S-gene Amplification Additional Kit
for low copy sample (K-2521)
Kit Components
① 1st S gene Full length RT-PCR (96 tests X 1 plate)
② S gene Fragment RT-PCR (24 tests X 4 plates)
Additional Kit for low copy sample (24 tests X 1 plate)
Workflow

104 copies 106 copies 102 copies
① 1st S gene Full length RT-PCR (K-2520) AccuPower® SARS-CoV-2 S gene Amplification
Additional Kit for low copy sample (K-2521)

② AccuPower® SARS-CoV-2 S gene Amplification Kit (K-2520)
S gene Fragment RT-PCR 

Cat. No. Product Description Specification
K-2520 AccuPower® SARS-CoV-2 S gene Amplification Kit 96 reactions
K-2521 AccuPower® SARS-CoV-2 S gene Amplification Additional Kit for low copy sample 24 reactions
Related Instruments
A-2041 AllInOneCycler™ PCR System -
A-7040 ExiSpin™ -

Step 5: PCR Clean-up
Cat. No. Product Description Specification
K-3037 AccuPrep® PCR/Gel Purification 200 reactions
K-3038 50 reactions

Step 6: Sanger Sequencing Service
Fast and cost-effective Bioneer's Sanger sequencing service
Cat. No. Product Description
N-8250 Sequencing Primer set for SARS-CoV-2 S gene Analysis
S-3010-15 Sequencing Service for S gene Analysis

* The primer is free if you use the Sanger sequencing service in Bioneer.

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  1. Rapid Purification of DNA fragments from various enzymatic reactants and agarose gel, PCR purification, gel purification
    AccuPrep® PCR/Gel Purification Kit
    As low as $60.00
  2. Vortexing & Spin-down system, vortex, spin down, vortexer, vortexing, centrifuge, table centrifuge
    ExiSpin™ [A-7040]
    $900.00
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