SARS-CoV-2 Mutation Research

COVID-19 virus are constantly evolving to new variants which have different shedding capacity and susceptibility to vaccine, which is mainly caused by the change of spike protein. To prevent the super-spreading of the variants, the S protein should be sequenced more quickly and efficiently. 
AccuPower^® SARS-CoV-2 S gene Amplification Kit provides the more fast and convenient solution for sequencing of S gene. The kit contains every RT-PCR components including primers and enzymes. Just add your RNA sample into 4 tubes of kit, you can amplify whole S gene for direct sanger sequencing.
You can also purchase an additional PCR kit to analyze the S-Protein gene sequence of fewer positive samples, such as 100 copies or less.
Direct Sanger Sequencing is possible after PCR purification without cloning.

Step 2: Viral RNA Extraction
Spin column type	Magnetic Bead type	Automatic type
AccuPrep® Viral RNA Extraction Kit	MagListo™ 5M Viral DNA/RNA Extraction Kit	ExiPrep™96 Lite
Cat. No. Product Description Specification K-3033 AccuPrep® Viral RNA Extraction Kit 100 reactions K-3624 MagListo™ 5M Viral DNA/RNA Extraction Kit 100 reactions TM-1011 MagListo™ 2-12h Magnetic Separation Rack 2 ml x 12 holes A-5250 ExiPrep™96 Lite -

Step 3: Amplification & Diagnosis
Diagnostics kit	Real-Time PCR
AccuPower® SARS-CoV-2 Multiplex Real-Time RT-PCR Kit	Exicycler™ 96/384 Real-Time Quantitative Thermal Block
Cat. No. Product Description A-2060-1 Exicycler™ 96 Real-Time Quantitative Thermal Block A-2061 Exicycler™ 384 Real-Time Quantitative Thermal Block

Step 4: S gene Amplification
AccuPower® SARS-CoV-2 S-gene Amplification Kit (K-2520)	AccuPower® SARS-CoV-2 S-gene Amplification Additional Kit for low copy sample (K-2521)
Kit Components
① 1st S gene Full length RT-PCR (96 tests X 1 plate) ② S gene Fragment RT-PCR (24 tests X 4 plates)	Additional Kit for low copy sample (24 tests X 1 plate)
Workflow
≤ 104 copies ≤ 106 copies ≤ 102 copies ① 1st S gene Full length RT-PCR (K-2520) ① AccuPower® SARS-CoV-2 S gene Amplification Additional Kit for low copy sample (K-2521) ② AccuPower® SARS-CoV-2 S gene Amplification Kit (K-2520) S gene Fragment RT-PCR
Cat. No. Product Description Specification K-2520 AccuPower® SARS-CoV-2 S gene Amplification Kit 96 reactions K-2521 AccuPower® SARS-CoV-2 S gene Amplification Additional Kit for low copy sample 24 reactions Related Instruments A-2041 AllInOneCycler™ PCR System - A-7040 ExiSpin™ -

Step 6: Sanger Sequencing Service

Fast and cost-effective Bioneer's Sanger sequencing service

Cat. No. Product Description N-8250 Sequencing Primer set for SARS-CoV-2 S gene Analysis S-3010-15 Sequencing Service for S gene Analysis * The primer is free if you use the Sanger sequencing service in Bioneer.