ExiPrep™96 Lite

ExiPrep™96 Lite is a instrument that automatically extracts nucleic acids using magnetic rods from up to 96 samples. Nucleic acid extraction is possible within less than 30 minutes in a small place (width 40 x height 58 cm).

Scientific Poster

• Automated Cell Free DNA Extraction using ExiPrep™96 Lite

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Cat. No.

A-5250(RUO)

Product Overview

Features and Benefits

Simultaneous sample extraction
Nucleic acid extraction from at least 32 to 96 samples by magnetic rods composed of 32 units, allowing usage without reagent waste
Built-in optimized nucleic acid extraction protocol
Built-in optimized nucleic acid extraction protocol for optimized kits and various samples, with a function for users to easily create their own protocol for special samples
Minimize nucleic acid extraction time
Quick nucleic acid extraction time only within 30 minutes
Wide compatibility

Nucleic acid extraction possible from various types of sample such as blood, bacteria, tissues, cells, plants, etc.
Prevention of contamination
Various parts such as UV lamp, contamination shield, etc. equipped to prevent contamination
Minimum workspace
Minimized equipment size and increased lab space efficiency

Technical/Specs

▶ Specifications

Physical specifications
Dimension (cm)	40(W) x 57.5(D) x 54.6(H)
Weight (kg)	46
Voltage / Frequency	100-240V~, 50/60Hz
Power	500VA Max (Fuse: 250V, F6:3AL, 2ea)
Operating specifications
Heating block	30-90℃
Temperature controlled block	4-90℃
Operating temperature	15-35℃
Operating humidity	20-80%, no condensation
Operating system	Built-in
Communication	TCP/IP
User interface Display	7 inch Touch
Data Storage	USB 2.0 (x2)

* Specifications of this product are subject to change without notice to improve product performance.

▶ Compatible Kits

Cat. No.	List of Kits
K-3603	MagListo™ 5M Genomic DNA Extraction Kit
K-3613	MagListo™ 5M Universal RNA Extraction Kit
K-3615	MagListo™ 5M Forensic Sample DNA Extraction Kit
K-3619	MagListo™ cfDNA Extraction Kit
K-3624	MagListo™ 5M Viral DNA/RNA Extraction Kit

* Reaction no. and product link can be accessed through [Ordering Info] tab.

▶ Experimental Data

(A) MagListo™ 5M Genomic DNA Extraction Kit

Genomic DNA Extraction from Human Whole Blood

Figure 1. Blood genomic DNA extraction using MagListo™ Genomic DNA Extraction Kit.
* Company Q Kit does not use RNase.
Lane 1: MagListo™ (Manual type, Bioneer)
Lane 2: ExiPrep™96 Lite (Bioneer)
Lane 3: Company Q (Manual type)
M: Bioneer 1 kb Ladder

Application on FFPE DNA Extraction

Figure 2. FFPE DNA extracted by using Manual method and ExiPrep™96 Lite. Gel electrophoresis and NanoDrop measurement of FFPE DNA. FFPE DNA was isolated with MagListo™ Genomic DNA Extraction Kit by manual method (lane 1-3) and ExiPrep™96 Lite (lane 4-11).

(B) MagListo™ 5M Universal RNA Extraction Kit

RNA Extraction from Animal Tissue (Mouse kidney)

Figure 3. Animal tissue RNA extraction using MagListo™ 5M Universal RNA Extraction Kit. RNA extraction from 20 mg of Mus musculus liver. DNase was not treated.
Lane 1: MagListo™ (Manual type, Bioneer)
Lane 2: ExiPrep™96 Lite (Bioneer)
Lane 3: Company Q (Manual type)

(C) MagListo™ 5M Forensic Sample DNA Extraction Kit

Comparison of real-time PCR data of extracted DNA
■ Sample : Urine 1 ~ 10 ml ■ Kit : MagListo™ 5M Forensic Sample DNA Extraction Kit, Company Q
■ Premix : AccuPower^® Plus DualStar™ qPCR PreMix (Cat.No. K-6600) ■ Target : GAPDH

Figure 4. DNA was extracted using MagListo™ 5M Forensic Sample DNA Extraction Kit and Company Q kit. DNA was extracted from 1 ~ 10 ml of Urine.

(D) MagListo™ cfDNA Extraction Kit

Comparison of cfDNA concentration purified with ExiPrep™96 Lite and competitor kit

Figure 5. Cell-free DNA was isolated from normal human serum using ExiPrep™96 Lite and Company T kit. DNA was quantified and visualized using Agilent 4150 TapeStation System.

(E) MagListo™ 5M Viral DNA/RNA Extraction Kit

Real-Time RT-PCR data of different genes extracted using ExiPrep™96 Lite

Figure 6. Real-Time RT-PCR data of E-gene/R-gene/infA/infB RNA in VTM media extracted using MagListo™ 5M Viral DNA/RNA Extraction Kit (Cat.No. K-3624) by ExiPrep™96 Lite. The regular results indicate that nucleic acid is extracted with high reproducibility.

▶ Selection Guide for each sample type

			K-3603	K-3613	K-3615	K-3619	K-3624
Sample Type	Cell		∨	∨
	Tissue		∨	∨
	Bacteria		∨
	Blood		∨
	Stool
	FFPE*		∨
	Swab						∨
	Sputum						∨
	Body fluid	Serum				∨	∨
		Plasma				∨	∨
		Saliva				∨
		Urine				∨
	Forensic sample	Dried body fluid spot			∨
		Hair			∨
		Bone & teeth			∨
		Chewing gum			∨
		Cigaratte butts			∨
		Buccal swab			∨

* FFPE DNA requires a pretreatment buffer.

Literature/Support

◈ Manuals

• User’s Guide_ExiPrep™ 96 Lite

• Onepage protocol_MagListo™ Genomic DNA Extraction Kit for ExiPrep™96 Lite

• Onepage protocol_MagListo™ Universal RNA Extraction Kit for ExiPrep™96 Lite

• Onepage protocol_MagListo™ Forensic Sample DNA Extraction Kit for ExiPrep™96 Lite

• Onepage protocol_MagListo™ cfDNA Extraction Kit for ExiPrep™96 Lite

• Onepage protocol_MagListo™ 5M Viral DNA/RNA Extraction Kit for Exiprep™96 Lite

◈ Brochure

• ExiPrep™ 96 Lite

• BIONEER - Instruments & Devices

• Automation of Nucleic acid extraction using ExiPrep™96 Lite

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 13485 - certificate

Ordering Info

▶ Ordering Infomation

Cat. No.	Product Description	Price
A-5250	ExiPrep™ 96 Lite	Inquire

▶ Related Products

Cat. No.	Product Description	Option
K-3603	MagListo™ 5M Genomic DNA Extraction Kit	100 reactions
K-3613	MagListo™ 5M Universal RNA Extraction Kit	100 reactions
K-3624	MagListo™ 5M Viral DNA/RNA Extraction Kit	100 reactions
K-3619	MagListo™ 5M cfDNA Extraction Kit	50 reactions
K-3615	MagListo™ 5M Forensic Sample DNA Extraction Kit	100 reacitons