Exicycler™ 96 V4 Real-Time Quantitative Thermal Block

Exicycler™ 96 V4 Real-Time Quantitative Thermal Block simultaneously reads 96-well using self-patented LT (Light Tunnel) technology which uniformly detects excited light, excitation light blocking technology and a 2D sensor. Those allow minimized optical sensitivity and deviation between wells during the real-time gene amplification. Furthermore, the installed thermal block is designed in standard 96-well format, allowing to be used with standard 0.2 ml PCR tube (white type) for high convenience.

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SKU
A-2060-1(RUO)

Overview

Optical Patent Technology

Bioneer patented fluorescence detection method (Korean Patent No. 10-1089045, US Patent No. US 8427643) was applied to minimize noise caused by reflected light, thus improving sensitivity and accuracy.

Figure.  Separation of a reflection light from fluorescence

* Noise caused by reflected light: The fluorescent materials used in qPCR create emission when the absorbed excitation energy excites them then falls to the ground state. In conventional qPCR instruments, part of excited light irradiated from the sample is reflected from the surface of the plate and the instrument, which may enter the detector to be also mistakenly read as part of the light from the sample. As the excited light has much more luminance than the fluorescent light, reflected light also contain high luminance, making it unable to be completely removed by the fluorescent light and creating noise in the process, deteriorating sensitivity and accuracy. However, by using our patented technology, excitation and fluorescence have different polarity, allowing the reflect light unable to be blocked by fluorescent filter to be filtered once again through polarizing filter. This allows drastic reduction of noise caused by reflected light compared with other qPCR instruments.

Features and Benefits

  • High reproducibility and accuracy

    Highly reproducible and accurate results by light tunnel (LT) technology (Korean Patent No. KR 0794703, US Patent No. 8139210, Japanese Patent JP 4751821, China Patent No. CN 1798969) irradiating uniform excitation light, excitation light interception method (Korean Patent No. 10-1089045 and US Pat. No. 8427643) using polarization, and a 96-well simultaneous detection technique using a 2-D sensor minimizing optical sensitivity and deviation between well

  • Reduce reaction time

    Maximum ramping rate of 4.5 ℃/sec (Exicycler ™ 96 Fast: max 5.0 ℃/sec) decreases reaction time by using a unique thermal block and temperature control technology

  • 5 color multiplexing system

    Simultaneous detection of five targets capable of being used in multiplex PCR without the need of reference dyes, used for the compensation of light quantity deviation, through the uniform optical system patent technology (Korean Patent KR 0794703, US Patent US 8139210, Japanese Patent JP 4751821, China Patent CN 1798969)

  • Convenience

    Diverse choice of fluorescent dyes including the universal ones with wide excitation/emission wavelength detection range being 475 to 690nm, unlike other traditional instruments designed for specific dyes 

  • Wide dynamic range

    Large detection range being more than 9 log allowing DNA detection and qualitative analysis even at low and high concentration samples using a short Arc lamp, a powerful excitation light source

  • Temperature gradient function

    Simplified search of optimal PCR annealing temperature with temperature gradients from 1~20°C available in the range of 20~95°C

  • Various application

    Wide applications such as touch down PCR through time increment and temperature increment adjustment functions

  • User-friendly software

    Accessible User-centric GUI for easy operation and manipulation for designing experiments and analyzing results

Application

• Gene expression analysis

• MicroRNA expression analysis

• Gene copy number analysis

• Pathogen detection

• Genetic mutation test

• GMO quantitative and qualitative test

Specifications

Dimension (cm) 35.5 (W) x 54 (D) x 47 (H)
Weight (kg) 39
Sample capacity/size Fast block: Opaque White 96-well Low Profile PCR Plate
/ 0.1 ml Opaque White 8-strip Low Profile PCR Tube
Normal block: Opaque White 96-well PCR Plate
/0.2 ml Opaque White 8-strip PCR Tube
Sample volume Fast block : 10 ~ 50 ㎕
Normal block : 20~100 ㎕ (50 ㎕ recommended)
Power consumption 100 - 240 VAC, 50/60 Hz, Max 850 VA
Operating temperature 15 ~ 35℃
Operating humidity 20 ~ 80%, no condensation
Thermo module specifications
Method of heating/cooling Peltier
Temperature range 4.0°C ~ 99.9°C
Max ramp rate Fast block : 5.0°C/sec
Normal block : 4.5°C/sec
Temperature accuracy ± 0.3°C
Temperature uniformity ± 0.3°C
Lid temperature 90 ~ 120°C
Temperature increment range 0.1°C ~ 2.0°C
Time increment range 1 sec ~ 60 sec
Gradient range 20°C ~ 95°C
Ramp rate control 1~100%
Computer specifications
Operating system Window 7 & 10 (32/64 bit OS)
Processor speed Intel Dual Core E2160 (1.8GHz) or higher
Memory 1GB or higher
Communication port USB 2.0 high speed
Screen resolution 1280X1024 or higher
Optical specifications
Light source Short arc lamp (120W)
Sensor 16 bit 2D CCD
Excitation filter/emission filter 5 sets*

※Specifications of this product are subject to change without notice to improve product performance.


Filter sets
Position Excitation (㎚) Emission (㎚) Set Applicable Fluorescent dye
1 Blank Blank 1  -
2 475 530 1  FAM, SYBR Green I
3 520 560 1  JOE, TET
4 550 590 1  TAMRA, CY3
5 570 630 1  Texas Red, ROX
6 630 690 1  CY5

Experimental data

Excellent uniformity

Figure 1. Fluorescence data using 106 copies of IRF3 gene (FAM labeled) in each of 96 well positions. The average Ct of 96 well is 21.8 and the Ct variation range is 0.19.



Wide linear dynamic range

Figure 2. Graph shows standard curve of tenfold serial dilutions of 10 copies to 1011 copies MMP9 gene (FAM labeled). The PCR efficiency generated by the standard curve is 103%.



Precise discrimination (1.33-fold dilutions)

Figure 3. Fluorescence data from a series of 1.33-fold dilutions of TMV gene (106 copies) amplified using reporter dyes to check one target: FAM/TMV. The PCR efficiency generated by the standard curve is 101%.



Real 5-color Multiplexing

Figure 4. 5 target genes can be detected in a single tube. (FAM:T. vaginalis, TET: M. Hominis, TAMRA: TMV, Texas Red: HSV type1, CY5: HSV type2)

Manual

Exicycler™ 96 (Ver.4)

Exicycler™ 96 (Ver.4) Fast


Brochure

Exicycler™ 96 (Ver.4)


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate


Cat. No. Product Description Price
A-2060-1 Exicycler™ 96 (Ver.4) Real-Time Quantitative Thermal Block Inquire
A-2060-2 Exicycler™ 96 (Ver.4) Fast Real-Time Quantitative Thermal Block Inquire

Related Products

Cat. No. Accessories
A-2060-A1 AccuPower® Fluorescence Test Kit for Exicycler™ 96
A-2060-A2 AccuPower® Fluorescence Test Kit for Exicycler™ 96 Fast
Cat. No. Premix & Reagent
K-6200 AccuPower® Greenstar™ qPCR PreMix, Exicycler 8-well strip, 50 µl, 12 strips, optical film included
K-6203 AccuPower® Greenstar™ qPCR PreMix, Exicycler 96-well plate, 50 µl, 1 plate, optical film included
K-6210 AccuPower® Greenstar™ qPCR PreMix, Exicycler 8-well strip, 20 µl, 12 strips, optical film included
K-6213 AccuPower® Greenstar™ qPCR PreMix, Exicycler 96-well plate, 20 µl, 1 plate, 20 µl, 1 plate, optical film included
K-6251 AccuPower® 2X Greenstar™ qPCR Master Mix with 80X ROX Dye / 100Rxn, 50 μL reaction
K-6252 AccuPower® 2X Greenstar™ qPCR Master Mix with 80X ROX Dye / 200Rxn, 50 μL reaction
K-6600 AccuPower® Plus DualStar ™ qPCR PreMix, Exicycler™ 96, 12 strips, Exicycler 8-well strip, 50 µl, optical film included, 96rxn
K-6704 AccuPower® Dual-HotStart ™ RT-qPCR PreMix, Exicycler™ 96, 12 strips, Exicycler 8-well strip, 50㎕, optical film included, 96 rxn
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