The SARS-CoV-2 virus has caused a number of infections around the world and continues to mutate. Variations that have increased infectivity due to various mutations, mutations that have increased mortality, and mutations that neutralize vaccines are emerging. Since these changes in virus characteristics are primarily affected by structural mutations in spike (S) proteins present on the surface of the virus, continuous tracking of changes in S-Protein is essential for effective control of COVID-19. AccuPower® SARS-CoV-2 S gene Amplification Kit is a one-step RT-PCR kit developed to easily and economically analyze the variation of S gene. The kit consists of four RT-PCR tubes per sample, so you can immediately add extracted RNA to analyze the genes of S-Protein with Sanger sequencing. You can also analyze the S-Protein gene sequence of fewer positive samples such as 100 copies or less using a Nested PCR kit. To quickly determine the variation of the S gene for SARS-CoV-2 positive samples, use this kit to perform RT-PCR quickly and accurately. And when you request the sequencing service of Bioneer, we analyze the sequence of S gene and provide the data. If you analyze the sequence of S-Protein yourself, you can purchase the sequencing primer set separately. The number of viruses is different for each sample of COVID-19 patients, so you need to use the appropriate kit for successful amplification. You can quickly and economically check the variation of S-protein by selecting the appropriate kits according to the virus copy of the estimated RNA extract through molecular diagnostic tests.
※This product is shipped in dry ice.
| Spin column type | Magnetic Bead type | Automatic type |
 AccuPrep® Viral RNA Extraction Kit |
 MagListo™ 5M Viral DNA/RNA Extraction Kit |
 ExiPrep™96 Lite |
| Cat. No. | Product Description | Specification |
| K-3033 | AccuPrep® Viral RNA Extraction Kit | 100 reactions |
| K-3624 | MagListo™ 5M Viral DNA/RNA Extraction Kit | 100 reactions |
| TM-1011 | MagListo™ 2-12h Magnetic Separation Rack | 2 ml x 12 holes |
| A-5250 | ExiPrep™96 Lite | - |
| Diagnostics kit | Real-Time PCR |
 AccuPower® SARS-CoV-2 Multiplex Real-Time RT-PCR Kit |
 Exicycler™ 96/384 Real-Time Quantitative Thermal Block |
| Cat. No. | Product Description |
| A-2060-1 | Exicycler™ 96 Real-Time Quantitative Thermal Block |
| A-2061 | Exicycler™ 384 Real-Time Quantitative Thermal Block |
| AccuPower® SARS-CoV-2 S gene Amplification Kit (K-2520) | |
| Kit Components | |
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| workflow | |
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| Cat. No. | Product Description | Specification |
| K-2520 | AccuPower® SARS-CoV-2 S gene Amplification Kit | ① 20 µl/rxn, 96 tests ② 20 µl/rxn, 24 tests |
| Related Instruments | ||
| A-2041 | AllInOneCycler™ PCR System | - |
| A-7040 | ExiSpin™ | - |
| Cat. No. | Product Description | Specification |
| K-3037 | AccuPrep® PCR/Gel Purification | 200 reactions |
| K-3038 | 50 reactions |
| Fast and cost-effective Bioneer's Sanger sequencing service |
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| Cat. No. | Product Description |
| N-8250 | Sequencing Primer set for SARS-CoV-2 S gene Analysis |
| Sequencing Service | |
* The primer is free if you use the Sanger sequencing service in Bioneer.
AccuPower® SARS-CoV-2 S gene Amplification kit contains all the required components for cDNA synthesis and PCR such as reverse transcriptase, DNA Polymerase, dNTPs, Reaction Buffer and etc, in the PreMix. Just add your RNA sample into 4-tubes of RT-PCR, your can amplify whole S gene for direct sanger sequencing.
For Low Copy Sample (>100 copies), provided additional Nested PCR kit for low copy sample.
The world's first Dual-HotStart™ RT-PCR reaction using PyroHotStart RT Reaction and HotStart PCR optimized to detect only the target gene preciesly.
Direct Sanger Sequencing is possible after PCR purification without cloning.
Reproducibility
Reproducible experimental results by mass production in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product.
• Full length Sanger sequencing for SARS-CoV-2 spike(S) gene
