EcoQprep™ mRNA Kit

EcoQprep™ mRNA Kit is a magnetic bead–based system for isolating high-quality mRNA with poly(A) tails from cultured cells, animal tissues, and plant tissues. Using oligo(dT) magnetic nanobeads and the EcoQprep™ Magnetic Separation Rack, the kit provides a faster and simpler workflow than traditional spin column methods. Because it does not require centrifugation, it reduces energy use and plastic waste. The purified mRNA is ready for downstream applications such as RT-PCR, RT-qPCR, cDNA synthesis, and gene cloning.

$380.00

Cat. No.

K-3708

Product Overview

Features

High-purity, high-yield mRNA isolation
Oligo(dT) magnetic nanobeads selectively capture polyadenylated mRNA, efficiently removing genomic DNA and ribosomal RNA to deliver highly pure results.
Eco-friendly nucleic acid extraction
Compared with conventional column filter methods, the magnetic bead–based workflow reduces plastic waste.
Broad sample compatibility
Enables high-quality mRNA extraction from diverse sources, including mammalian cells, animal tissues, and plant tissues.
Rapid processing time
Magnetic bead technology allows mRNA extraction in approximately 10 minutes*
Minimal equipment required
Only theEcoQprep™ Magnetic Separation Rack(TM-1012) is needed—no centrifuge or additional instruments are required.
Automation compatible
Fully compatible with the ExiPrep™ 96 Lite (A-5250, RUO) automated nucleic acid extraction system, enabling simultaneous processing of up to 96 samples.

^*excluding sample pretreatment

Applications

• cDNA synthesis

• Reverse transcription PCR(RT-PCR)

• Reverse transcription quantitative PCR(RT-qPCR)

• Northern blot analysis

• RNA sequencing

Technical/Specs

▶ Specifications

Technial Specifications
Expected Total RNA Yield (mRNA typically accounts for 1–5% of total RN)	~ 100 μg
Minimum Elution Volume	15 μL
Extraction Time	~ 10 min

* Results may differ according to sample types.

RNA Extraction Efficiency by Sample Type
Sample	Recommended Input Amount	Total RNA Yield (mRNA typically 1 - 5 %)	Purity*
Cultured Animal Cells	10⁴ - 10⁸ cells	15 - 20 μg	A₂₆₀/A₂₈₀ > 2.0
Animal Tissue (Liver)	25 - 50 mg	10 - 60 μg
Animal Tissue (Spleen)	100 mg	30 - 60 μg
Plant Tissue	100 mg	70 - 80 μg

▶ Components

Components	Volume	Quantity*
MRD Buffer	30 mL	1 ea
MRW1 Buffer	40 mL	1 ea
MRW2 Buffer	50 mL	1 ea
MRW3 Buffer	50 mL	1 ea
EM Buffer	25 mL	1 ea
Magnetic Nanobead-Oligo dT (10 mg/mL)	3 mL	1 ea
1.5 mL Tube	50 ea	1 pack
One Page Protocol	-	1 ea

* Mini-50 rxn

▶ Experimental Data

Table 1. Yield and purity of total RNA and mRNA purified from various samples.

Sample		Yield (μg)	Purity (A₂₆₀/A₂₈₀)	mRNA : total RNA Ratio (%)
HeLa Cell (2 x 10⁵ cells)	Total RNA	9.31	2.10	3.14
HeLa Cell (2 x 10⁵ cells)	mRNA	0.29	2.12	3.14
Mouse Liver (10 mg)	Total RNA	19.06	2.17	3.86
Mouse Liver (10 mg)	mRNA	0.74	2.16	3.86
Mouse Brain (10 mg)	Total RNA	25.13	2.10	2.00
Mouse Brain (10 mg)	mRNA	0.50	2.23	2.00
Broccoli (40 mg)	Total RNA	8.69	1.97	1.82
Broccoli (40 mg)	mRNA	0.16	1.97	1.82

Total RNA was purified using the AccuPrep^® Universal RNA Extraction Kit, and polyadenylated mRNA was purified using the EcoQprep™ mRNA Kit.
RNA yield (µg) and purity (A₂₆₀/A₂₈₀) were measured with a spectrophotometer. The mRNA ratio in total RNA (%) was calculated from the proportion of mRNA yield relative to total RNA yield. Samples tested included HeLa cells (2 × 10⁵ cells), mouse liver (10 mg), mouse brain (10 mg), and broccoli (40 mg).

Total RNA	mRNA	Number of HeLa Cells	Total RNA	mRNA
		2 x 10⁵	20.98	20.60
		2 x 10⁴	25.04	24.57
		2 x 10³	29.05	28.13
		2 x 10²	24.85	31.90
		2 x 10¹	UD	35.01
		2 x 10⁰	UD	37.51

Figure 1. Comparison of real-time two-step RT-qPCR amplification of HPRT1 from HeLa cells using total RNA and mRNA purification kits.

HeLa cells (2 × 10⁵ – 2 × 10⁰ cells) were used as input samples. Total RNA was purified with the AccuPrep^® Universal RNA Extraction Kit, and polyadenylated mRNA was purified with the EcoQprep™ mRNA Kit. Amplification of the HPRT1 gene was performed by two-step RT-qPCR.
[Total RNA] Amplification curves obtained from total RNA.
[mRNA] Amplification curves obtained from mRNA.
[Table] Ct values of HPRT1 detected from serial dilutions of HeLa cells. UD = undetermined.

Literature/Support

◈ Manuals

• User Guide - EcoQprep™ mRNA Kit(EN)

• One Page Protocol - EcoQprep™ mRNA Kit

◈ MSDS

• MSDS - EcoQprep™ mRNA Kit MRD Buffer EN.pdf

• MSDS - EcoQprep™ mRNA Kit MRW1 Buffer EN.pdf

• MSDS - EcoQprep™ mRNA Kit MRW2 Buffer EN.pdf

• MSDS - EcoQprep™ mRNA Kit MRW3 Buffer EN.pdf

• MSDS - EcoQprep™ mRNA EM Buffer EN.pdf

• MSDS - EcoQprep™ mRNA Magnetic Nano Bead-Oligo dT Buffer EN.pdf

◈ Brochure/Flyer

• EcoQprep™ Series (EN)

• EcoQprep™ mRNA Kit Flyer

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

Ordering Info

▶ Ordering Information

Cat. No.	Product	Volume
K-3708	EcoQprep™ mRNA Kit	50 reactions

▶Related Products

Cat. No.	Product	Volume
TM-1012	EcoQprep™ Magnetic Separation Rack	2 mL tube x 12 holes
TM-1021	EcoQprep™ Magnetic Separation Rack	15 mL tube x 6 holes
TM-1031	EcoQprep™ Magnetic Separation Rack	50 mL tube x 3 holes