AccuNanoBead™ Ni-NTA Magnetic Beads, size 400nm (0.5 g/25 ml)

Magnetic beads are used as materials for cell experiments, DNA purification and disease observation, and can also be used as materials for direct treatment. This AccuNanoBead™ have the advantages of high Binding Capacity due to their relatively large surface area because they are nano-sized.

$510.00

Cat. No.

TA-1017-1

Product Overview

Overview

Recently, there have been many researches applying magnetic beads in the field of biology: from cellular experiments and DNA purification to disease monitoring and even disease treatment. The surface of magnetic beads are coated with functional groups that allows binding of DNA, RNA, proteins, and specific cells. Afterwards, the beads are moved or attached to a desired position through external magnetic fields to perform purification. In addition, while conventional Magnetic Beads are micro-sized, this AccuNanoBeads™ is nano-sized, bearing the advantages of high binding capacity due to their relatively large surface area.

Features and Benefits

Miniscule size
Average sizes of 400 nm magnetic nanobeads
Uniformed size distribution
Sphere form of silica coated magnetic nanobeads
Excellent protein purification efficiency
Rapid purification

Application

• Magnetic DNA/RNA Purification

• Magnetic Protein Purification

• Magnetic Biosimilar Separation

• Magnetic Antibody Separation

Technical/Specs

▶ Protein Purification Method by using Magnetic Nanobeads

Affter loading the protein sample to the tube, add the magnetic beads to combine them. Afterwards, fix the beads by using external magnetic field and wash the solution. Finally, elute the DNA or RNA from the magnetic beads. This method provides faster DNA purification than traditional ones.

Figure 1. Recombinant Protein and Antibody protein purification protocol by Magnetic Nanobeads

▶ FE-SEM and TEM Images of Magnetic Nanobeads

Figure 2. FE-SEM picture of Magnetic Nanobeads. The electron microscope picture shows spherical magnetic nanobeads.

▶ EDS Analysis of Magnetic Nanobeads

Figure 3. EDS analysis results of Magnetic Nanobeads. The results show that the Magnetic Nanobeads consist of silica and iron oxide.

▶ Size Distribution of Magnetic Nanobeads

Figure 4. Particle size distribution of Magnetic Nanobeads. The average size of the Magnetic Nanobeads is about 400 nm.

▶ Comparison of Protein Purification Beads

Figure 5.
(a) SEM image of 450 nm Bioneer Magnetic Nanobeads
(b) SEM image of A company Magnetic Beads
(c) SEM image of B company Magnetic Beads
(d) SEM image of C company Magnetic Beads
(e) SEM image of D company Magnetic Beads

Figure 5 shows scanning electron microscope images of magnetic beads from Bioneer and a different company (b~e). Scanning electron microscope image of synthesized magnetic nanobeads shows that magnetic nanobeads of Bioneer is 400 nm size and spherical shape. However, the magnetic beads from the other company are micro meter size and various shape.

▶ Protein Purification Yield

Table 1 shows the protein purification yield of Bioneer Ni-NTA magnetic nanobeads. From these results, it can be seen that Bioneer magnetic nanobeads have a high protein purification yield of 77%. The protein used in the experiment was a 17kDa His-Tagged protein. The yield is the amount of target protein purified from the loading protein.
Table 1

Table 1. Protein purification yield of bioneer Ni-NTA magnetic nanobeads(30 mg).

Figure 6. SDS-PAGE image of purified 17 kDa His-tagged protein with Bioneer Ni-NTA magnetic nanobeads.

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

• EN ISO 13485 - certificate

Ordering Info

▶ Ordering Information

Magnetic Nanobeads are coated for various functions and sold for research and separation.

Cat. No.	Product Description	Option	Price
TA-1010-1	AccuNanoBead ™ Silica Magnetic Nanobeads, size 400nm	0.5 g/25 ml	$TA-1010-1
TA-1010-2		1.0 g/50 ml	$TA-1010-2
TA-1010-3		10 g/500 ml	$TA-1010-3
TB-1010-1	AccuNanoBead ™ Silica Magnetic Nanobeads, size 200nm	0.5g/25ml/EA	$TB-1010-1
TB-1012-1	AccuNanoBead ™ COOH Magnetic Nanobeads, size 400 nm	0.5 g	$TB-1012-1
TA-1011-1	AccuNanoBead ™ NH2 Magnetic Nanobeads, size 400 nm	0.5 g	$TA-1011-1
TA-1012-1	AccuNanoBead ™ COOH Magnetic Nanobeads, size 400 nm	0.5 g	$TA-1012-1
TA-1013-1*	AccuNanoBead ™ Epoxy Magnetic Nanobeads, size 400 nm	0.5 g	$TA-1013-1
TA-1014-1	AccuNanoBead ™ C18 Magnetic Nanobeads, size 400 nm	0.5 g	$TA-1014-1
TA-1015-1*	AccuNanoBead ™ Streptavidin Magnetic Nanobeads, size 400 nm	50 mg/5 ml	$TA-1015-1
TB-1015-1*	AccuNanoBead ™ Streptavidin Magnetic Nanobeads, size 200 nm	50 mg/5 ml	$TB-1015-1
TA-1016-1*	AccuNanoBead ™ Biotin Magnetic Nanobeads, size 400 nm	0.5 g/25 ml	$TA-1016-1
TA-1017-1	AccuNanoBead ™ Ni-NTA Magnetic Nanobeads, size 400 nm	0.5 g/25 ml	$TA-1017-1
TA-1019-1	AccuNanoBead ™ Thiol Magnetic Nanobeads, size 400 nm	0.5 g	$TA-1019-1
TA-1021-1	AccuNanoBead ™ Protein G Magnetic Nanobeads, size 400nm	40 ml/ 1 ml, 1 ea	$TA-1021-1
TA-1021-5		40 ml/ 1 ml, 5 ea	$TA-1021-5
TA-1021-10		40 ml/ 1 ml, 10 ea	$TA-1021-10
TA-1022-1	AccuNanoBead ™ Protein A Magnetic NanoBeads, size 400 nm	40 ml/ 1 ml, 1 ea	$TA-1022-1
TA-1022-5		40 ml/ 1 ml, 5 ea	$TA-1022-5
TA-1022-10		40 ml/ 1 ml, 10 ea	$TA-1022-10
TF-1012-1	COOH Magnetic Beads for NGS	500 mg	$TF-1012-1
TF-1012-2		1 g	$TF-1012-2
TF-1012-3		4 g	$TF-1012-3
TS-1000-1	Magnet Φ15x1.5 and Screw Tube 1.5 ml	1 set	$TS-1000-1

* It is shipped in ice packing.

** Please inquiry for bulk orders.

▶ Related Products

Cat. No.	Product Description	Option
K-7250	AccuRapid ™ Cell-Free Protein Expression Kit	45 ul x 24 reactions
K-7300	ExiProgen™ EC Protein Synthesis Kit	16 reactions
K-7301		32 reactions
K-7302		96 reactions
D-2010	AccuLadder™ Protein Size Marker (Broad)	500 ul
D-2020	AccuLadder™ Protein Size Marker (Low)	500 ul
K-7220	ExiProgen™ His-tagged Protein Purification Kit	16 reactions
K-7200	ExiProgen™ His-tagged Protein Purification Kit	5 reactions

▶ Technical Support and Ordering

For more information on our products, please contact us by email.
nano-support@bioneer.com