AccuNanoBead™ Ni-NTA Magnetic Beads, size 400nm (0.5 g/25 ml)
Magnetic beads are used as materials for cell experiments, DNA purification and disease observation, and can also be used as materials for direct treatment. This AccuNanoBead™ have the advantages of high Binding Capacity due to their relatively large surface area because they are nano-sized.
Recently, there have been many researches applying magnetic beads in the field of biology: from cellular experiments and DNA purification to disease monitoring and even disease treatment. The surface of magnetic beads are coated with functional groups that allows binding of DNA, RNA, proteins, and specific cells. Afterwards, the beads are moved or attached to a desired position through external magnetic fields to perform purification. In addition, while conventional Magnetic Beads are micro-sized, this AccuNanoBeads™ is nano-sized, bearing the advantages of high binding capacity due to their relatively large surface area.
Features and Benefits
Average sizes of 400 nm magnetic nanobeads
Uniformed size distribution
Sphere form of silica coated magnetic nanobeads
Excellent protein purification efficiency
• Magnetic DNA/RNA Purification
• Magnetic Protein Purification
• Magnetic Biosimilar Separation
• Magnetic Antibody Separation
▶ Protein Purification Method by using Magnetic Nanobeads
Affter loading the protein sample to the tube, add the magnetic beads to combine them. Afterwards, fix the beads by using external magnetic field and wash the solution. Finally, elute the DNA or RNA from the magnetic beads. This method provides faster DNA purification than traditional ones.
Figure 1. Recombinant Protein and Antibody protein purification protocol by Magnetic Nanobeads
▶ FE-SEM and TEM Images of Magnetic Nanobeads
Figure 2. FE-SEM picture of Magnetic Nanobeads. The electron microscope picture shows spherical magnetic nanobeads.
▶ EDS Analysis of Magnetic Nanobeads
Figure 3. EDS analysis results of Magnetic Nanobeads. The results show that the Magnetic Nanobeads consist of silica and iron oxide.
▶ Size Distribution of Magnetic Nanobeads
Figure 4. Particle size distribution of Magnetic Nanobeads. The average size of the Magnetic Nanobeads is about 400 nm.
▶ Comparison of Protein Purification Beads
(a) SEM image of 450 nm Bioneer Magnetic Nanobeads
(b) SEM image of A company Magnetic Beads
(c) SEM image of B company Magnetic Beads
(d) SEM image of C company Magnetic Beads
(e) SEM image of D company Magnetic Beads
Figure 5 shows scanning electron microscope images of magnetic beads from Bioneer and a different company (b~e). Scanning electron microscope image of synthesized magnetic nanobeads shows that magnetic nanobeads of Bioneer is 400 nm size and spherical shape. However, the magnetic beads from the other company are micro meter size and various shape.
▶ Comparison of Protein Purification Yield
The protein purification yields of Bioneer Ni-NTA Magnetic Nanobeads and other company magnetic beads are listed in Table 1. His-Tagged DNA Polymerase proteins of the 97 kDa expressed in E. coli were used in experiment. Purification yield represents the amount of purified target protein from loading protein. In Table1, it indicate that the purification yield of Bioneer Magnetic Nanobeads is higher than that of four different company Magnetic Beads. In addition, purity of protein purification was more than 90%. Bioneer Magnetic Nanobeads have the highest purification yields compared to 4 different Magnetic Beads because the surface area of Magnetic Beads increases with size.
Table 1. Comparison of protein purification yield of Bioneer beads and other company beads .
Figure 6. Comparison of elution protein of Bioneer beads and other company beads..
Figure 7. SDS-PAGE image of purified 94 kDa DNA Polymerase with Bioneer Magnetic Nanobeads.
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
▶ Ordering Information
Magnetic Nanobeads are coated for various functions and sold for research and separation.
|Cat. No.||Product Description||Option||Price|
|TA-1010-1||AccuNanoBead ™ Silica Magnetic Nanobeads, size 400nm||0.5 g/25 ml||$250.00|
|TA-1010-2||1.0 g/50 ml||$450.00|
|TA-1010-3||10 g/500 ml||$2,550.00|
|TA-1011-1||AccuNanoBead ™ NH2 Magnetic Nanobeads, size 400 nm||0.5 g||$280.00|
|TA-1012-1||AccuNanoBead ™ COOH Magnetic Nanobeads, size 400 nm||0.5 g||$290.00|
|TA-1013-1||AccuNanoBead ™ Epoxy Magnetic Nanobeads, size 400 nm||0.5 g||$310.00|
|TA-1014-1||AccuNanoBead ™ C18 Magnetic Nanobeads, size 400 nm||0.5 g||$310.00|
|TA-1015-1||AccuNanoBead ™ Streptavidin Magnetic Nanobeads, size 400 nm||50 mg/5 ml||$250.00|
|TA-1016-1||AccuNanoBead ™ Biotin Magnetic Nanobeads, size 400 nm||0.5 g/25 ml||$310.00|
|TA-1017-1||AccuNanoBead ™ Ni-NTA Magnetic Nanobeads, size 400 nm||0.5 g/25 ml||$510.00|
|TA-1019-1||AccuNanoBead ™ Thiol Magnetic Nanobeads, size 400 nm||0.5 g||$380.00|
|TA-1021-1||AccuNanoBead ™ Protein G Magnetic Nanobeads, size 400nm||40 mg/1 ml, 1 ea||$152.00|
|TA-1021-5||40 mg/1 ml, 5 ea||$522.00|
|TA-1021-10||40 mg/1 ml, 10 ea||$800.00|
|TA-1022-1||AccuNanoBead ™ Protein A Magnetic NanoBeads, size 400 nm||40 mg/1 ml, 1 ea||$152.00|
|TA-1022-5||40 mg/1 ml, 5 ea||$522.00|
|TA-1022-10||40 mg/1 ml, 10 ea||$800.00|
|TF-1012-1||COOH Magnetic Beads for NGS||500 mg||$590.00|
|TS-1000-1||Magnet Φ15x1.5 and Screw Tube 1.5 ml||1 set||$5.00|
*Please inquiry for bulk orders.
▶ Related Products
|Cat. No.||Product Description||Option|
|K-7250||AccuRapid ™ Cell-Free Protein Expression Kit||45 ul x 24 reactions|
|K-7300||ExiProgen™ EC Protein Synthesis Kit||16 reactions|
|D-2010||AccuLadder™ Protein Size Marker (Broad)||500 ul|
|D-2020||AccuLadder™ Protein Size Marker (Low)||500 ul|
|K-7220||ExiProgen™ His-tagged Protein Purification Kit||16 reactions|
▶ Technical Support and Ordering
For more information on our products, please contact us by email.