AccuNanoBead™ Protein G Magnetic Nanobeads, size 400nm
AccuNanoBead™ Protein G Magnetic Nanobeads contains silica magnetic nanobeads coated with high-purity (> 95%) of Protein G. 1 ml of bead solution can purify various antibodies including 1.2 mg or more of Human IgG and is compatible with Bead of ExiProgen Kit. It can be applied to antibody purification, immunoprecipitation, antigen-antibody interaction studies, protein complex studies, cell separation, etc.
Features and Benefits
- Reduced experimental time
Rapid purification with minimized loss through powerful magnetism of Magnetic Nanobeads
- High binding capacity
Large surface area of Magnetic Nanobeads with average diameter of 400nm
- Strong specificity
Reduced non-specific binding through the use of homogeneous spherical nanobeads
• Antibody purification
• Antigen-Antibody interaction studies
• Protein complex studies
• Cell separation
|Protein G magnetic nano beads||1mL X 1ea (4 %)||2 - 8°C Storage|
|Concentration||40 mg/ml (4% suspension)|
|Max. Binding Capacity||> 30 μg Human IgG/mg beads sample|
|Storage and Stability||Beads can be stored at 2 - 8°C until the expiration date.
Do not freeze and vortex vigorously.
▶ Binding affinity table
▶ Experiment Results
Figure 1. Comparison of protein binding efficiency of AccuNanoBead ™ Protein G Magnetic Nanobeads with a competitor.
A) Eluted fraction after binding of Human IgG followed by washing through Protein G magnetic bead from Bioneer (AccuNanoBead™) or from a competitor L. Upper and lower bands are heavy (HC) and light chain (LC) of purified Human IgG in SDS-PAGE gel, respectively.
B) Comparison of binding capacity in Bioneer’s AccuNanoBead ™ with competitor’s. Amount of bound IgG is quantified by UV-spectrophotometer. All reaction is carried out for 1 hour at room temperature. Human IgG is purchased from Sigma (Cat. no. I4506).
- M: protein marker (Bioneer, Cat. No. D-2020)
- Bioneer: AccuNanoBead™ Protein G Magnetic Nanobeads.
Figure 2. IgG purification of AccuNanoBead ™ Protein G Magnetic Nanobeads from human whole blood
IgG was purified from human whole blood. Arrows point to heavy and light chains of IgG, respectively.
- M: protein marker
- W: human whole blood
- U: unbound fraction
- E1 & 2: elution fraction 1 & 2.
|Cat. No.||Product Description||Option||Price|
|TA-1021-1||AccuNanoBead ™ Protein G Magnetic Nanobeads, size 400 nm||40 mg/1 ml, 1 ea||$152.00|
|TA-1021-5||40 mg/1 ml, 5 ea||$522.00|
|TA-1021-10||40 mg/1 ml, 10 ea||$800.00|
The BIONEER products for recombinant protein synthesis and purification are categorized as the following, Those can be selected according to the purpose.
To apply for the Exiprogen™ demo, plase contact email@example.com .
|Process||Product type||Equipment used||Service|
Syn / Puri / Dialysis