AccuNanoBead™ Protein G Magnetic Nanobeads, size 400nm

AccuNanoBead™ Protein G Magnetic Nanobeads contains silica magnetic nanobeads coated with high-purity (> 95%) of Protein G. 1 ml of bead solution can purify various antibodies including 1.2 mg or more of Human IgG and is compatible with Bead of ExiProgen Kit. It can be applied to antibody purification, immunoprecipitation, antigen-antibody interaction studies, protein complex studies, cell separation, etc.

※This product is shipped with ice pack.

As low as $152.00

Cat. No.

Product Overview

Features and Benefits

Reduced experimental time
Rapid purification with minimized loss through powerful magnetism of Magnetic Nanobeads
High binding capacity
Large surface area of Magnetic Nanobeads with average diameter of 400nm
Strong specificity
Reduced non-specific binding through the use of homogeneous spherical nanobeads

Application

• Antibody purification

• Immunoprecipitation

• Antigen-Antibody interaction studies

• Protein complex studies

• Cell separation

Technical/Specs

▶ Components

Components		Storage Temperature
Protein G magnetic nano beads	1mL X 1ea (4 %)	2 - 8°C Storage
Manual	1 ea	2 - 8°C Storage

▶ Specifications

Feature	Specification
Matrix	Silica-coated Fe₃O₄
Concentration	40 mg/ml (4% suspension)
Max. Binding Capacity	> 30 μg Human IgG/mg beads sample
Storage and Stability	Beads can be stored at 2 - 8°C until the expiration date. Do not freeze and vortex vigorously.

▶ Binding affinity table

▶ Experiment Results

Figure 1. Comparison of protein binding efficiency of AccuNanoBead ™ Protein G Magnetic Nanobeads with a competitor.
A) Eluted fraction after binding of Human IgG followed by washing through Protein G magnetic bead from Bioneer (AccuNanoBead™) or from a competitor L. Upper and lower bands are heavy (HC) and light chain (LC) of purified Human IgG in SDS-PAGE gel, respectively.
B) Comparison of binding capacity in Bioneer’s AccuNanoBead ™ with competitor’s. Amount of bound IgG is quantified by UV-spectrophotometer. All reaction is carried out for 1 hour at room temperature. Human IgG is purchased from Sigma (Cat. no. I4506).
- M: protein marker (Bioneer, Cat. No. D-2020)
- Bioneer: AccuNanoBead™ Protein G Magnetic Nanobeads.

Figure 2. IgG purification of AccuNanoBead ™ Protein G Magnetic Nanobeads from human whole blood
IgG was purified from human whole blood. Arrows point to heavy and light chains of IgG, respectively.
- M: protein marker
- W: human whole blood
- U: unbound fraction
- E1 & 2: elution fraction 1 & 2.

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

Cat. No.	Product Description	Option	Price
TA-1021-1	AccuNanoBead ™ Protein G Magnetic Nanobeads, size 400 nm	40 mg/1 ml, 1 ea	$TA-1021-1
TA-1021-5		40 mg/1 ml, 5 ea	$TA-1021-5
TA-1021-10		40 mg/1 ml, 10 ea	$TA-1021-10

* It is shipped in ice packing.

Selection Guide

The BIONEER products for recombinant protein synthesis and purification are categorized as the following, Those can be selected according to the purpose.

To apply for the ExiProgen™ demo, plase contact sales@bioneer.co.kr .

Process

Product type

Equipment used

Service

DNA Template

Preparation