AccuTarget™ Human Premade siRNA Set

We provide 25 Gene Family and Pathway-specific siRNA Library Sets for research on various cellular processes, cancer, and diseases. Related siRNAs can be ordered in 0.1, 0.25, 0.5, 1 nmole Guaranteed Yield.

Cat. No.	Pathway Name	No. of Genes	No. of siRNAs
SHS-0010	Antioxidant	38	114
SHS-0020	Apoptosis	290	870
SHS-0250	Cancer	1,157	3,471
SHS-0030	Caspase	37	111
SHS-0040	Cell cycle	112	336
SHS-0050	Cyclase	21	63
SHS-0060	Cytochrome	52	156
SHS-0070	Deaminase	22	66
SHS-0080	GPCR signaling pathway	727	2,181
SHS-0090	Helicase	114	342
SHS-0100	Isomerase	104	312
SHS-0110	Kinase	699	2,097
SHS-0120	Ligase	272	816

Cat. No.	Pathway Name	No. of Genes	No. of siRNAs
SHS-0130	Lyase	123	369
SHS-0140	Motor	122	366
SHS-0150	NF-kB	37	111
SHS-0160	Nucleic acid binding	2,573	7,719
SHS-0170	Oxidoreductase	551	1,653
SHS-0180	Peptidase	491	1,473
SHS-0190	Phosphatase	188	564
SHS-0200	Receptor	1,516	4,548
SHS-0210	Transferase	1,428	4,284
SHS-0220	Transporter	1,021	3,063
SHS-0230	Tubulin	20	60
SHS-0240	Ubiquitin	77	231

Product Overview

Overview

Bioneer provides various siRNA library sets according to gene families and pathway classifications. All siRNAs are synthesized in a clean room and supplied via our Bio-RP purification system. Futhermore, Bioneer's Premade Library can directly undergo high-throughput analysis into the functional class of interest. Libraries specific to the certain research areas can be also easily found.

• AccuTarget^TM Human Premade siRNA Set

We provide 25 gene families and pathway-specific siRNA library sets for various cellular processes, cancer, and disease related researches.

• AccuTarget^TM Human Premade siRNA Subset

The siRNA subset library is a functional family subset of kinase or phosphatase libraries.

• AccuTarget^TM Human siRNA Druggable siRNA Library Set

This product is a human druggable siRNA library consisting of 8,175 gene targets known as disease-causing genomes.

Features and Benefits

Guaranteed qualified products

All siRNAs are synthesized in a clean room and supplied via our Bio-RP purification system
Highly customizable synthesis

Choices for scale (10, 20, 50, 100 nmole) and provide Pathway-specific siRNA Library Sets for research on various cellular processes, cancer, and diseases
Reasonable price
Maximized efficiency compared with customer's research costs

Technical/Specs

▶ Experimental data

All synthesized siRNAs are meticulously tested in quality through MALDI-TOF mass spectrometry while their the duplex structures are confirmed by PAGE analysis (Figure. 1, 2).

Figure 1. MALDI-TOF mass spectrometry analysis of a custom siRNA. All siRNAs are processed by MALDI-TOF mass spectrometry to ensure its quality.

Figure 2. PAGE data of double-stranded custom siRNA. Complementary single-stranded RNA strands were hybridized to form siRNA duplex and analyzed by 15% non-denaturing PAGE.
- SS: single-stranded RNA
- DS: double-stranded siRNA

Figure 3. Knockdown efficiency of AccuTarget ™ siRNA Library. To determine the knockdown efficiency, HeLa cells were transfected with individual siRNAs at 100 nM concentration. 24 hours post-transfection, total RNA was isolated and the level of target mRNA was measured by qRT-PCR. This data demonstrates the effectiveness of the Turbo si-Designer algorithm: 83.8% of tested siRNAs induced > 70% knockdown and 38.1% of tested siRNAs elicited > 90% knockdown.

Figure 4. Knockdown efficiency of AccuTarget ™ Human Validated Cell Cycle siRNA set. HeLa cells were transfected with individual siRNAs targeting 33 different genes at a concentration of 20 nM. Total RNAs was isolated and the level of target mRNA was measured by qRT-PCR. This data demonstrates the effectiveness of the Turbo si-Designer algorithm.

Literature/Support

◈ Manual

• AccuTarget™ Genome-wide Predesigned siRNA library

• Annealing of single-stranded RNA for siRNA

• AccuTarget™ Real-Time PCR Primer Library

◈ Brochure

• siRNA Products and services

• All about Bioneer's Custom Services

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

We provide AccuTarget™ Premade Human siRNA Library, consisting of 25,368 siRNAs designed for immediate experimental use. These pre-made siRNA libraries can be ordered at 10, 20, 50 or 100 nmole Guaranteed Yield. We can also offer 25 pathway-specific siRNA library sets for various cellular processes, cancer, and disease related researches, which can be ordered at 0.1, 0.25, 0.5, or 1 nmole Guaranteed Yield, and 21 siRNA pre-validated libraries with proven knockdown efficiency, which can be ordered at 10, 20, 50, or 100 nmole Guaranteed Yield.

▶ siRNA Set

Bioneer provides siRNA library sets for 25 different gene families and pathways for various cellular processes, cancer, and disease related research purposes. These siRNAs can be ordered at 0.1, 0.25, 0.5, or 1 nmole Guaranteed Yield.

Cat. No.	Product Description	No. of Genes	No. of siRNAs
SHS-0010	AccuTarget ™ Human Antioxidant siRNA set	38	114
SHS-0020	AccuTarget ™ Human Apoptosis siRNA set	290	870
SHS-0250	AccuTarget ™ Human Cancer siRNA set	1,157	3,471
SHS-0030	AccuTarget ™ Human Caspase siRNA set	37	111
SHS-0040	AccuTarget ™ Human Cell cycle siRNA set	112	336
SHS-0050	AccuTarget ™ Human Cyclase siRNA set	21	63
SHS-0060	AccuTarget ™ Human Cytochrome P450 siRNA set	52	156
SHS-0070	AccuTarget ™ Human Deaminase siRNA set	22	66
SHS-0080	AccuTarget ™ Human GPCR signaling pathway siRNA set	727	2,181
SHS-0090	AccuTarget ™ Human Helicase siRNA set	114	342
SHS-0100	AccuTarget ™ Human Isomerase siRNA set	104	312
SHS-0110	AccuTarget ™ Human Kinase siRNA set	699	2,097
SHS-0120	AccuTarget ™ Human Ligase siRNA set	272	816
SHS-0130	AccuTarget ™ Human Lyase siRNA set	123	369
SHS-0140	AccuTarget ™ Human Motor siRNA set	122	366
SHS-0150	AccuTarget ™ Human NF-kB pathway siRNA set	37	111
SHS-0160	AccuTarget ™ Human Nucleic acid binding siRNA set	2,573	7,719
SHS-0170	AccuTarget ™ Human Oxidoreductase siRNA set	551	1,653
SHS-0180	AccuTarget ™ Human Peptidase siRNA set	491	1,473
SHS-0190	AccuTarget ™ Human Phosphatase siRNA set	188	564
SHS-0200	AccuTarget ™ Human Receptor siRNA set	1,516	4,548
SHS-0210	AccuTarget ™ Human Transferase siRNA set	1,428	4,284
SHS-0220	AccuTarget ™ Human Transporter siRNA set	1,021	3,063
SHS-0230	AccuTarget ™ Human Tubulin siRNA set	20	60
SHS-0240	AccuTarget ™ Human Ubiquitin siRNA set	77	231

▶ siRNA Subset

The siRNA subset library is a functional family subset of the kinase or phosphatase library. These siRNAs can be ordered at 0.1, 0.25, 0.5, or 1 nmole Guaranteed Yield.

Cat. No.	Product Description	No. of Genes	No. of siRNAs
SHS-0110-1	AccuTarget ™ Human AGC Kinase siRNA Subset	58	174
SHS-0110-2	AccuTarget ™ Human CAMK Kinase siRNA Subset	65	195
SHS-0110-3	AccuTarget ™ Human CK Kinase siRNA Subset	11	33
SHS-0110-4	AccuTarget ™ Human CMGC Kinase siRNA Subset	56	168
SHS-0110-5	AccuTarget ™ Human Other Kinase siRNA Subset	342	1,026
SHS-0110-6	AccuTarget ™ Human STE Kinase siRNA Subset	43	129
SHS-0110-7	AccuTarget ™ Human TK Kinase siRNA Subset	84	252
SHS-0110-8	AccuTarget ™ Human TKL Kinase siRNA Subset	40	120
SHS-0190-1	AccuTarget ™ Human Other Phosphatase siRNA Subset	147	441
SHS-0190-2	AccuTarget ™ Human Tyrosine Phosphatase siRNA Subset	41	123

▶ siRNA Druggable Set

This set can be ordered at 0.1, 0.25, 0.5, or 1 nmole Guaranteed Yield.

Cat. No.	Product Description	No. of Genes	No. of siRNAs
SHD-00XX	AccuTarget ™ Human Druggable siRNA library set	8,175	24,525