AccuTarget™ Human GPCR signaling pathway siRNA set

All synthesized siRNAs are meticulously tested in quality through MALDI-TOF mass spectrometry while their the duplex structures are confirmed by PAGE analysis (Figure. 1, 2).

Figure 1. MALDI-TOF mass spectrometry analysis of a custom siRNA. All siRNAs are processed by MALDI-TOF mass spectrometry to ensure its quality.

Figure 2. PAGE data of double-stranded custom siRNA. Complementary single-stranded RNA strands were hybridized to form siRNA duplex and analyzed by 15% non-denaturing PAGE.
- SS: single-stranded RNA
- DS: double-stranded siRNA

Figure 3. Knockdown efficiency of AccuTarget ™ siRNA Library. To determine the knockdown efficiency, HeLa cells were transfected with individual siRNAs at 100 nM concentration. 24 hours post-transfection, total RNA was isolated and the level of target mRNA was measured by qRT-PCR. This data demonstrates the effectiveness of the Turbo si-Designer algorithm: 83.8% of tested siRNAs induced > 70% knockdown and 38.1% of tested siRNAs elicited > 90% knockdown.

Figure 4. Knockdown efficiency of AccuTarget ™ Human Validated Cell Cycle siRNA set. HeLa cells were transfected with individual siRNAs targeting 33 different genes at a concentration of 20 nM. Total RNAs was isolated and the level of target mRNA was measured by qRT-PCR. This data demonstrates the effectiveness of the Turbo si-Designer algorithm.

We provide AccuTarget™ Premade Human siRNA Library, consisting of 25,368 siRNAs designed for immediate experimental use. These pre-made siRNA libraries can be ordered at 10, 20, 50 or 100 nmole Guaranteed Yield. We can also offer 25 pathway-specific siRNA library sets for various cellular processes, cancer, and disease related researches, which can be ordered at 0.1, 0.25, 0.5, or 1 nmole Guaranteed Yield, and 21 siRNA pre-validated libraries with proven knockdown efficiency, which can be ordered at 10, 20, 50, or 100 nmole Guaranteed Yield.

▶ siRNA Set

Bioneer provides siRNA library sets for 25 different gene families and pathways for various cellular processes, cancer, and disease related research purposes. These siRNAs can be ordered at 0.1, 0.25, 0.5, or 1 nmole Guaranteed Yield.

▶ siRNA Subset

The siRNA subset library is a functional family subset of the kinase or phosphatase library. These siRNAs can be ordered at 0.1, 0.25, 0.5, or 1 nmole Guaranteed Yield.

▶ siRNA Druggable Set

This set can be ordered at 0.1, 0.25, 0.5, or 1 nmole Guaranteed Yield.