Bioneer has world-class infrastructure and know-how, and all the procedures of qPCR experiment using it follow the guideline of MIQE† (Minimum Information for Publication of Quantitative Real-Time PCR Experiments). The whole process is done by experienced professionals, so you can get accurate and reliable results quickly.
Bioneer’s gene expression analysis service provides high quality data following *MIQE Guideline designed for Real-Time PCR experts. Accurate results can be quickly gained and readily presented to your paper. Those are verified with papers published by our past customers.
*The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) “qPCR gene expression analysis” Agreed for quality control of papers: Bustin, S.A., et al. 2009. The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments, Clinical Chemistry 55:4, 611-622.Precise primer designs, synthesis, and tests are crucial for accurate Real-Time PCR data. Even for experienced researchers, considerable amounts of time and money must be spent for making good primers as those require not only suitable designs, but also verification tests. With our continuous researches over the last 10 years, our Gene Expression Assay Team has acquired over 12,000 pairs of proven primer sets with strong specificity and sensitivity that are capable of producing high quality data. Furthermore, no matter what gene analysis that a customer orders, our proven experts having years of experiences can swiftly build and test the best primers to provide the gene synthesis analysis data as quickly as possible. For the improved accuracy, we undergo a primer test to see whether target specific amplification is possible. If the result does not meet the standard, we will start the whole process again from the beginning.
Figure 1. Time required for primer synthesis and verification
A) A single target gene: Minimum 4 business days B) Multiple target genes: 4-7 business days
1Order and Consultation
Please fill out “qPCR Array Service Order Form” according to the instruction written on the ‘Ordering Protocol' Tab, then send the completed form to the Gene Expression Analysis Team by email. (qPCRarray@bioneer.com)
qPCR Array Service Download order form
2Sample Shipping
Various types of samples can be shipped to our company in number of ways to prevent degradation of the RNA for us to analyze. For more information, see the instructions on the ‘Ordering Protocol’ tab.
3Total RNA extraction and gDNA removal
According to the type of requested samples, we first choose the suitable high quality, high purity RNA extraction kit to undergo total RNA extraction. Moreover, to eliminate gDNAs that affects mRNA quantification, we treat the sample with RNase free DNase I.
4Quality Check
All the impurities in the extracted total RNA may affect the amplification efficiency by inhibiting the PCR polymerase reaction. Therefore, spectrophotometers are used for measuring both the quantity and purity of the nucleic acids. Since it is difficult to accurately quantify the amount of degraded RNA, capillary gel electrophoresis is also used to measure the RNA quality score (RQS). All those measurements will be recorded in the report and sent to the customers. We will proceed to the next step only if the purity and the degree of degradation of the RNA satisfy the recommended value (RQS > 8.0).
*If the customer wants, the qPCR service will be still available for samples even for those that do not satisfy the RQS standards. However, Bioneer will not guarantee the reliability of the derived data.
Figure 1. RNA quality check example. Caliper LabChip™ GX Results
5cDNA synthesis
cDNA synthesis is an important step along with RNA quality check for the gene expression analysis as its efficiency is greatly influenced by the mRNA. By using Bioneer’s patent technologies of Thermostable RTase (RocketScript™) and Cyclic Temperature Reverse Transcription (CTRT), full-length cDNA can be synthesized, even with RNAs having complex secondary structures, for accurate expression analysis. The RT primers used may vary from Oligo dT and Random primer(dN6, dN12) depending on the purpose of the customer’s research purposes.
Figure 2. Comparison of cDNA synthesis efficiency between Bioneer's and other companies'
6qPCR
Bioneer's qPCR Array Service is performed by experienced experts in qPCR experiments. It is a high-sensitivity detection optical system and has been patented Exicycler™ 96 Real-Time Quantitative Thermal Cycler is used to produce highly reliable qPCR data. All equipment is regularly inspected and managed by ISO regulations.
Figure 3. (A) Exicycler™ 96 Real-Time Quantitative Thermal Block (B) Amplification Curve
7Analyzing and providing results
2-ΔΔCt method is used for carrying out relative quantitative analysis using qPCR. Excel file will be sent to the customers with the following data: raw Ct values, melting curve analysis, p-value, fold change, scatter plots, volcano plots, and heat map image. qPCR raw data can be provided if Exicycler™ analysis software is installed. Information on primers such as genetic information, amplicon size, and PCR conditions following the MIQE can be also provided upon the customer’s request.
Figure 4. qPCR analysis result
▶ qPCR Array Service Order
▶ qPCR Array Service Time required
| Number of analyzed genes | Service deadline |
| ≤ 16 genes | 2 weeks |
| ≤ 32 genes | 2 ~ 3 weeks |
| ≤ 96 genes | 3 ~ 5 weeks |
| Tissue sample request |
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| Cell sample request |
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| Request blood and other samples |
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| Required sample amount | * Depending on your experimental design, we may ask for more.
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| Precautions |
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| Required sample amount | * Depending on your experimental design, we may ask for more.
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▶ Sending the samples
qPCR Array Service provides accurate quantitative results for many genes by providing a combination of quantitative PCR with high sensitivity and reliability for gene expression analysis and microarray technique for analyzing expression patterns of several genes at once.
Please refer to the How to Order tab. Then, click the qPCR Array Service Order Form qPCR Array Service Order Form and send it to qPCRarray@bioneer.com . We will immediately guide you to consultation and quotation.
In the 'How to Order' tab, you can refer to the note on sample request.
1) RNA extraction request
2) Total RNA sample request
3) cDNA sample request
1. qPCR array service report (pdf file)
Provides workflow-specific results and fold change values for the entire qPCR Array Service.
2. Fold change analysis file (excel file)
Provides the calculation sheet recorded with the analyzed fold change values such as raw Ct, dCt, 2-dCt , fold change, etc., applied with a relative quantification 2-ΔΔCt method.
3. qPCR array service raw data (excel file)
Provides qPCR results with the analyzed raw data such as PCR protocol, Ct, Ct Threshold, Baseline, Tm values, etc., through Exicycler analysis program.
4. qPCR array service Interpret results Help (pdf)
Provides guidelines on qPCR array services and result analysis.
You don’t need to. As long as you provide an accurate information for the samples, we will undergo the analysis after the primer design and validation steps.
We also have an addidional service called "DNase I treatment." Although we only apply the servive for those requesting the RNA sample analysis, it is provided as default when requesting qPCR array services from the RNA extraction.
1. Check your Gene ID
1) Search by target gene symbol in NCBI Gene (https://www.ncbi.nlm.nih.gov/gene/).
2) Gene ID is the number found at the bottom of the gene name. Check the Gene name and Description to make sure it is correct.
2. Find Accession number
1) Search by target gene symbol in NCBI Gene (https://www.ncbi.nlm.nih.gov/gene/).
2) After selecting the gene name, under 'mRNA and Protein(s)' category, you can see the mRNA and protein accession number in the category.
The relative analysis 2-ddCt method uses a reference gene to normalize the extraction yield, reverse transcription yield, amplification efficiency, and the RNA quantity. The selected gene must be able to undergo stable expression and closely related to the total RNA contents of the samples. Also, as selecting the reference genes are highly important to get accurate qPCR results for each experiment, we advise choosing them based on the related papers and experiment results. If you find this step difficult, we provide an additional service for reference gene screening. Refer to AccuPower® qPCR Array System: Reference qPCR primer Set page.
| Angiogenesis PCR Library |
Angiogenesis PCR Library is for researching the expression of key genes involved with modulating the biological processes of angiogenesis by including includes growth factors and their receptors, chemokines and cytokines, matrix and adhesion molecules, proteases and their inhibitors, as well as transcription factors, all involved in the development of new blood vessels.
· Click here for gene information |
| Apoptosis PCR Library |
Apoptosis PCR Library is for researching the expression of key genes involved with programmed cell death which has critical role in normal biological processes requiring cell removal including differentiation, development, and homeostasis.
· Click here for gene information |
| Cancer Drug Target, Metabolism and Resistance PCR Library |
Cancer Drug Target, Metabolism and Resistance PCR Library is for researching the expression of key genes involved in the body's response to chemotherapy, such as important enzymes in drug resistance (P-glycoproteins), phase I metabolism (specifically the P450 family), and phase II metabolism (such as various covalent modification enzymes).
· Click here for gene information |
| Cancer Mechanism and pathway PCR Library |
Cancer Mechanism and Pathway PCR Library is for researching the expression of key gene involved in the major tumorigenesis and transformation mechanism of cancer, such as cell cycle control, DNA damage repair, apoptosis, cell senescence, signal transduction, transcription factors, adhesion, angiogenesis, invasion and metastasis.
· Click here for gene information |
| Oncogenes and Tumor Suppressor Genes PCR Library |
Oncogenes and Tumor Suppressor Genes PCR Library is for researching the expression of key genes involved with promotion of oncogenesis, oncogenic or tumor suppressor properties finding the correlation with biological phenotypes or clinical outcomes like staging, therapy selection, metastasis, recurrence, and survival rate, among others.
· Click here for gene information |
| Tumor Metastasis PCR Library |
Tumor Metastasis PCR Library is for researching the expression of key genes involved with in metastasis by including several classes of protein factors including cell adhesion, ECM components, cell cycle, cell growth and proliferation, apoptosis, transcription factors and regulators and other genes related to tumor metastasis.
· Click here for gene information |
| Adipogenesis PCR Library |
Adipogenesis PCR Library is for researching the expression of key genes involved in the differentiation and maintenance of mature adipocytes which have multi function, such as storing energy, doing as dynamic endocrine organ, hormone and cytokine (adipokine) secretion.
· Click here for gene information |
| Blood Coagulation PCR Library |
Blood Coagulation PCR Library is for researching the expression of key genes involved in blood coagulation processes, including factors in many blood disorders such as peripheral arterial and cardiovascular disease, sickle cell anemia, anti-thrombin deficiencies, von Willebrand disease (vWD) and many other conditions associated with thrombosis and platelet aggregation defects.
· Click here for gene information |
| Cardiogenesis-promoting Factor PCR Library |
Cardiogenesis-promoting Factor PCR Library is for researching the expression of key genes involved in heart development including the process of cell specification, cell differentiation, cell migration, morphogenesis and interactions among cells from several embryonic origins.
· Click here for gene information |
| Cardiomyocyte Differentiation PCR Library |
Cardiomyocyte Differentiation PCR Library is for researching the expression of key genes involved in cardiac organogenesis.
· Click here for gene information |
| Cell Cycle and Regulators PCR Library |
Cell Cycle and Regulators PCR Library contains genes that both positively and negatively regulate the cell cycle, the transitions between the each of the phases, DNA replication, checkpoints and arrest. Using real-time PCR, you can easily and reliably analyze expression.
· Click here for gene information |
| Cell Lineage Identity PCR Library |
Cell Lineage Identity PCR Library is for researching the expression of key genes involved in cellular differentiation. Analysis with the genes could suggest the cellular status of an intermediately or terminally differentiated cell, and/or the mechanism of a studied differentiation process.
· Click here for gene information |
| Cell Motility and Regulators PCR Library |
Cell Motility and Regulators PCR Library is for researching the expression of key genes involved in the movement of cells.
· Click here for gene information |
| Cell Surface Markers PCR Library |
Cell Surface Markers profiling PCR Library contains genes for researching the expression of cell surface markers. Analysis results from these genes can be used as cell-type markers, subtype characterization or organ transplantation studies.
· Click here for gene information |
| Cellular Senescence PCR Library |
Cellular Senescence PCR Library is for researching the expression of key genes involved in the initiation and progression of the biological process causing cells to lose the ability to divide.
· Click here for gene information |
| CYP450 and other Oxygenases PCR Library |
CYP450 and other Oxygenases PCR Library is for researching the expression of key genes participating in the CYP450 containing systems including the cytochrome oxidoreductase and the cytochrome b5 reductases. Moreover, these include the functionally related members of the monooxygenase, monoamine oxygenase and lipooxygenase families.
· Click here for gene information |
| Cytoskeleton Regulators PCR Library |
Cytoskeleton Regulators PCR Library is for researching the expression of key genes involved in controlling the intracellular scaffolding's biogenesis, organization, polymerization, and depolymerization.
· Click here for gene information |
| Differentiation Markers PCR Library |
Differentiation Markers PCR Library is for researching the expression of key genes which are important for the identification of specific cell types.
· Click here for gene information |
| DNA Methylation and Transcriptional Repression PCR Library |
DNA Methylation and Transcriptional Repression PCR Library is for researching the expression of key genes involved in transcriptional repression mechanism in the precise control of gene expression.
· Click here for gene information |
| Endothelial Cell PCR Library |
Endothelial Cell PCR Library is for researching the expression of key genes involved in endothelial cell biology which is critical in the progression of disease processes including inflammation, atherosclerosis, and tumor angiogenesis.
· Click here for gene information |
| Epigenetic Chromatin Remodeling Regulators PCR Library |
Epigenetic Chromatin Remodeling Regulators PCR Library is for researching the expression of key genes involved with recognizing chromatin modifications and remodeling, enzymes known or predicted to modify genomic DNA, histones to regulate chromatin accessibility and therefore gene expression.
· Click here for gene information |
| Epithelial to Mesenchymal Transition and Regulators PCR Library |
Epithelial to Mesenchymal Transition and Regulators PCR Library is for researching the expression of key genes involved in either change their expression during this process or regulate those gene expression changes.
· Click here for gene information |
| Extracellular Matrix and Adhesion Molecules PCR Library |
Extracellular Matrix and Adhesion Molecules PCR Library is for researching the expression of key genes important for cell-cell and cell-matrix interactions.
· Click here for gene information |
| Glycosylation Regulators PCR Library |
Glycosylation Regulators PCR Library is for researching the expression of key genes involved in encoding enzymes that post-translationally add and remove sugar residues to and from proteoglycans and glycoproteins.
· Click here for gene information |
| Guidance Cues, Growth Cone Motility and Regulators PCR Library |
Guidance Cues, Growth Cone Motility and Regulators PCR Library is for researching the expression of key genes involved in promoting the initiation, extension, stabilization or retraction of individual filopodia, or the capture or stabilization of microtubules in specific regions of the neuronal growth cone.
· Click here for gene information |
| Heat Shock Proteins and Regulators PCR Library |
Heat Shock Proteins PCR Library is for researching the expression of key genes encoding heat shock proteins (HSPs, or molecular chaperones) which are important components of cellular networks.
· Click here for gene information |
| Ligand Gated Ion Channels and Regulators PCR Library |
Ligand Gated Ion Channels and Regulators PCR Library is for researching the expression of key genes encoding ion channel family members responding to chemical signal (the ligand).
· Click here for gene information |
| Mitochondria Metabolism PCR Library |
Mitochondria Metabolism PCR Library is for researching the expression of key genes involved in the biogenesis and function of the cell's powerhouse organelle. It includes regulators and mediators of mitochondrial molecular transport of not only the metabolites needed for the electron transport chain and oxidative phosphorylation, but also the ions required for maintaining the mitochondrial membrane polarization and potential important for ATP synthesis.
· Click here for gene information |
| Osteogenesis PCR Library |
Osteogenesis PCR Library is for researching the expression of key genes involved in osteogenic differentiation which is critical for development of the skeletal system as well as bone mineral metabolism.
· Click here for gene information |
| Phosphodiesterase Pathway PCR Library |
Phosphodiesterase Pathway PCR Library is for researching the expression of key genes encoding Phosphodiesterases. Its activity is cleaving a variety of substrates, such as phosphodiester bonds in second messenger molecules cAMP and cGMP.
· Click here for gene information |
| Polycomb, Trithorax Complexes and Regulators PCR Library |
Polycomb, Trithorax Complexes and Regulators PCR Library is for researching the expression of key genes encoding polycomb and trithorax complex components including chromatin modification enzymes and remodeling factors.
· Click here for gene information |
| Primary Cilia and Regulators PCR Library |
Primary Cilia and Regulators PCR Library is for researching the expression of key genes important for ciliary organization and maintenance.
· Click here for gene information |
| Skeletal Muscle PCR Library |
Skeletal Muscle PCR Library is for researching the expression of key genes involved in skeletal muscle differentiation, function and disease-related processes.
· Click here for gene information |
| Sperm Motility PCR Library |
Sperm Motility PCR Library is for researching the expression of key genes involved in sperm cell motility and behavior.
· Click here for gene information |
| Stress Response and Regulators PCR Library |
Stress Response and Regulators PCR Library is for researching the expression of key genes involved in cellular stress response, such as encoding antioxidant and pro-oxidant enzymes, molecular chaperones and proteins involved in xenobiotic metabolism.
· Click here for gene information |
| Synaptic Plasticity and Regulators PCR Library |
Synaptic Plasticity and Regulators PCR Library is for researching the expression of key genes involved in synaptic alterations during learning and memory.
· Click here for gene information |
| Telomeres, Telomerase and Regulators PCR Library |
Telomeres, Telomerase and Regulators PCR Library are for researching the expression of key genes involved in telomere replication and maintenance.
· Click here for gene information |
| Transcription Factors PCR Library |
Transcription Factors PCR Library is for researching the expression of key genes directly controlling when, where, and the extent to which genes are expressed.
· Click here for gene information |
| Ubiquitination and Regulators PCR Library |
Ubiquitination and Regulators PCR Library is for researching the expression of key genes involved in regulated degradation of cellular proteins or foreign proteins by the ubiquitin-proteasome system.
· Click here for gene information |
| Ubiquitin-Proteasome Dependent Proteolysis PCR Library |
Ubiquitin-Proteasome Dependent Proteolysis PCR Library is for researching the expression of key genes involved in ubiquitin degradation process.
· Click here for gene information |
| Unfolded Protein and Regulators PCR Library |
Unfolded Protein and Regulators PCR Library is for researching the expression of key genes involved in the recognizing and responding to misfolded protein accumulation in the endoplasmic reticulum (ER). Misfolded proteins and chaperon complex in ER induce extreme stress by initiating protein kinase cascades which cause inhibiting ER translation, reverse ER translocation, and activating ubiquitination enzymes. This signaling pathway is also involved in transcriptional factors synthesis increasing the expression of heat shock proteins, protein disulfide isomerases and more chaperones. Moreover, it influences glycosylation enzymes regulating ER protein folding quality control and the sensor recognizing, and transcriptional factors responding to cholesterol biosynthesis dysregulation.
· Click here for gene information |
| Voltage-Gated Ion Channels PCR Library |
Voltage-Gated Ion Channels PCR Library is for researching the expression of genes encoding the component of transmembrane ion channels which have a critical role in neurons as well as in other types of cells.
· Click here for gene information |
| Wound Healing PCR Library |
Wound Healing PCR Library is for researching the expression of key genes involved in wound healing processes which pass through three overlapping phases; inflammation, granulation and tissue remodeling.
· Click here for gene information |
| Alopecia PCR Library |
Alopecia PCR Library is for researching the expression of key genes Alopecia that means a condition in which hair is lost from some or all areas of the body including immunological processes such as interleukin signaling, skin related genes such as the keratins, and apoptosis/apoptosis inhibition related factors
· Click here for gene information |
| ALS Pathogenesis PCR Library |
ALS Pathogenesis PCR Library is for researching the expression of key genes involved in ALS (Amyotrophic Lateral Sclerosis) which is a devastating neurodegenerative disorder..
· Click here for gene information |
| Alzheimer's Disease PCR Library |
Alzheimer's Disease PCR Library is for researching the expression of key genes involved in onset, development and progression of Alzheimer’s disease.
· Click here for gene information |
| Asthma and Allergy PCR Library |
Asthma and Allergy PCR Library is for researching the expression of key genes involved in allergic responses and asthma caused by over-representation of the T helper 2 (Th2) cell subtype and allergic inflammation is commonly characterized by the IgE Ab isotype. Both the cell and the molecule play central roles in allergic disease mechanisms.
· Click here for gene information |
| Atherosclerosis PCR Library |
Atherosclerosis PCR Library is for researching the expression of key genes involved in atherosclerosis including the factor of blood coagulation and circulation, cell-adhesion, lipid transport and metabolism, stress response, cell growth and proliferation, and apoptosis.
· Click here for gene information |
| Autism PCR Library |
Autism PCR Library is for researching the expression of key genes involved in a brain development disorder including the factor of neurodevelopment, neurotransmitters, cell adhesion molecules, and cell signal transduction.
· Click here for gene information |
| Breast Cancer PCR Library |
Breast Cancer PCR Library is for researching the expression of key genes involved in the dysregulation of signal transduction and other normal biological processes during breast carcinogenesis and in breast cancer cell lines.
· Click here for gene information |
| Cardiac Hypertrophy PCR Library |
Cardiac Hypertrophy PCR Library is for researching the expression of key genes involved in myocardium responding to a wide array of intrinsic and extrinsic stimuli, including hypertension, myocardial infarction, cardiac arrhythmias, valvular disease, endocrine disorders, increased workload, injury, and contractile abnormalities resulting from mutant sarcomeric proteins by hypertrophic growth.
· Click here for gene information |
| Cholera PCR Library |
Cholera PCR Library is for researching the expression of key genes involved in life cycles of cholera which induces vomiting, hypovolemic shock and acidosis.
· Click here for gene information |
| Colorectal Cancer and Metastasis PCR Library |
Colorectal Cancer and Metastasis PCR Library is for researching the expression of key genes involved in colorectal tumors arisen as a result of the mutational activation of oncogenes coupled with the mutational inactivation of tumor suppressor genes without a major role for gene amplification or rearrangement.
And also, it contains metastatical state arisen as a result of the mutational activation of oncogenes coupled with the mutational inactivation of tumor suppressor genes without a major role for gene amplification or rearrangement. · Click here for gene information |
| Diabetes PCR Library |
Diabetes PCR Library is for researching the expression of key genes related to the onset, development, and progression of diabetes.
· Click here for gene information |
| Fibrosis PCR Library |
Fibrosis PCR Library is for researching the expression of key genes involved in dysregulated tissue remodeling during the repair and healing of wounds which have three distinctive phases; inflammation, granulation and tissue remodeling.
· Click here for gene information |
| HIV Infection and Immunity PCR Library |
HIV Infection and Immunity PCR Library is for researching the expression of key genes involved in the host response to HIV infection by including the genes involved in innate and adaptive immunity related to HIV infection along with the key cellular cofactors for HIV replication.
· Click here for gene information |
| Huntington's Disease PCR Library |
Huntington's Disease PCR Library is for researching the expression of key genes directly or potentially involved in Huntington's disease (HD).
· Click here for gene information |
| Hypertension PCR Library |
Hypertension PCR Library is for researching the expression of key genes from biological pathways regulating blood vessel constriction and dilation in response to a variety of signals.
· Click here for gene information |
| Liver Cancer PCR Library |
Liver Cancer PCR Library is for researching the expression of key genes involved in the progression of hepatocellular carcinoma (HCC), as well as other forms of hepatocarcinogenesis.
· Click here for gene information |
| Lymphoma and Leukemia PCR Library |
Lymphoma and Leukemia PCR Library is for identifing the expression patterns of specific genes and signaling pathways known to be involved or implicated in human hematopoietic neoplasms as well as in normal hematopoietic differentiation and function.
· Click here for gene information |
| Mood Disorder PCR Library |
Mood Disorder PCR Library is for researching the expression of key genes for monitoring gene expression or copy number variation associated with bipolar affective disorder, but also contains significant gene overlap with schizophrenia and other mood disorders.
· Click here for gene information |
| Multiple Sclerosis PCR Library |
Multiple Sclerosis PCR Library is for researching the expression of key genes involved in multiple sclerosis (MS) progression which is an autoimmune disease of the central nervous system, progressively destroys axonal myelin sheaths and oligodendrocytes. Symptoms primarily result from the loss of myelin and accompanying inflammation.
· Click here for gene information |
| Neurodegeneration PCR Library |
Neurodegeneration PCR Library is for researching the expression of genes that have been identified as important in many neurodegenerative diseases including Alzheimer's, Parkinson's, Huntington's, ALS, prion disease and others.
· Click here for gene information |
| Obesity PCR Library |
Obesity PCR Library is for researching the expression of key genes associated with obesity-related genes that are directly involved in the regulation of energy intake and expenditure. These genes encode for orexigenic and anorectic peptides, hormones, receptors , central and peripheral signaling molecules involved in energy expenditure.
· Click here for gene information |
| Osteoporosis PCR Library |
Osteoporosis PCR Library is for researching the expression pattern of genes implicated in osteoporosis and osteogenesis by monitoring the Wnt and TGF- β signaling pathways which play an important role in the formation of bone and bone mineral density.
· Click here for gene information |
| Pancreatic Adenocarinoma PCR Library |
Pancreatic Adenocarinoma PCR Library is for researching the expression of key genes involved in pancreatic ductal adenocarcinoma evolved from a progressive cascade of cellular, morphological and architectural changes from normal ductal epithelium through pre-neoplastic lesions termed PanIN (Pancreatic Intraepithelial Neoplasia).
· Click here for gene information |
| Parkinson's Disease PCR Library |
Parkinson's Disease PCR Library is for researching the expression of key genes directly or potentially involved in Parkinson's disease (PD) which is neurodegenerative disorder caused by loss of dopaminergic neurons.
· Click here for gene information |
| Prion PCR Library |
Prion PCR Library is for researching the expression of key genes involved in prion disease, which is highly fetal neurodegenerative disorder, is caused by the toxic prion protein(PrP).
· Click here for gene information |
| Prostate Cancer PCR Library |
Prostate Cancer PCR Library is for researching the expression of key genes involved in prostate cancer development including deregulated genes detected routinely in molecular analysis of prostate cancer samples and in high-throughput microarray profiling studies, as well as genes known to have differentially methylated promoters in prostate cancer.
· Click here for gene information |
| Rheumatoid Arthritis PCR Library |
Rheumatoid Arthritis PCR Library is for researching the expression of key genes involved in RA (Rheumatoid Arthritis), which is a chronic debilitating autoimmune disease of unknown etiology affecting diarthrodial joints. Hallmarks of the synovial abnormalities in RA are synovial lining cell proliferation, neoangiogenesis, and inflammatory cell infiltration including the myeloid, macrophage, and lymphoid lineages.
· Click here for gene information |
| Antigen Presentation and Processing PCR Library |
Antigen Presentation and Processing PCR Library is for researching the expression of key genes which is important for dendritic cell activation and maturation, such as cytokines, chemokines, their receptors are included on this array along with other related cell surface receptors and signal transduction molecules.
· Click here for gene information |
| Antiviral Immune Response PCR Library |
Antiviral Immune Response PCR Library is for researching the expression of key genes involved in the innate antiviral immune responses. Anti-viral innate immune response is triggered by pattern recognition receptors (PRRs), such as toll-like (TLRs), Nod-like (NLRs), and RIG-I-like receptors, resulting in inducing the expression of inflammatory cytokines including alpha and beta interferons.
· Click here for gene information |
| Autophagy PCR Library |
Autophagy PCR Library is for researching the expression of key genes involved in autophagy, an intracellular catabolic process that destroys a cell's own damaged proteins and organelles via the lysosome.
· Click here for gene information |
| B cell Signaling Pathway PCR Library |
B Cell Signaling Pathway PCR Library is for researching the expression of key genes involved in B cell activation and signaling that occurs through the B cell receptor (BCR).
· Click here for gene information |
| Chemokine and Receptors PCR Library |
Chemokine and Receptors PCR Library is for researching the expression of key genes encoding chemokines and their receptors, such as members of the C-C and C-X-C subfamilies and their receptors as well as other related genes.
· Click here for gene information |
| Cytokine and Receptors PCR Library |
Cytokine and Receptors PCR Library is for researching the expression of key genes encoding important cytokines, such as interferons and interleukins, bone morphogenetic proteins (BMP), members of the TGF-ß family, platelet-derived and vascular endothelial growth factors, tumor necrosis factors.
· Click here for gene information |
| FoxP3 and Regulators PCR Library |
FoxP3 and Regulators PCR Library is for researching the expression of key genes involved in FoxP3 signaling transduction which is selectively expressed in T regulatory cells and is a master regulator of their development and function.
· Click here for gene information |
| Helper T Cell Differentiation and Regulators PCR Library |
Helper T Cell Differentiation and Regulators PCR Library is for researching the expression of key genes regulating the commitment of precursor T cells to differentiate into specific effector subtypes including the gene of cytokine, cytokine receptor, transcription factor, and other signaling molecule regulating differentiation into Th1 or Th2 cells as well as specific markers for these subtypes.
· Click here for gene information |
| Immune Complement PCR Library |
Immune Complement PCR Library is for researching the expression of key genes involved in immune complement which is major components of the classical, alternative and lectin pathways, including accessory molecules and multiple complement control proteins.
· Click here for gene information |
| Immune System Phenotyping and Regulators PCR Library |
Immune System Phenotyping and Regulators PCR Library is for researching the expression of immune cell surface markers for discriminating the major immune cell lineages, such as B-cells, T-cells, NK cells, mast cells, basophils, red blood cells, dendritic cells and macrophages. Markers are also included for many lineage subtypes, such as marginal zone B cells, B1 B cells, T-regs, Th-17, Th1, Th2 and NKT cells.
· Click here for gene information |
| Immunological NF-κB Signaling Pathway PCR Library |
Immunological NF-κB Signaling Pathway PCR Library is for researching the expression of key genes involved in the activation and signaling of NF-κB.
· Click here for gene information |
| Immunology PCR Library |
Immunology PCR Library is for researching the expression of key genes involved inthe immune system and immune related disorders including signaling molecules, apoptotic mediators, immune complement, cytokines / chemokines (and their receptors), MHC molecules, interferon response genes, secreted lytic molecules, immunoglobulins, immune transcription factors and surface molecules.
· Click here for gene information |
| Inflammation and Regulators PCR Library |
Inflammation and Regulators PCR Library is for researching the expression of key genes involved in inflammatory response.
· Click here for gene information |
| Innate and Adaptive Immune Response PCR Library |
Innate and Adaptive Immune Response PCR Library is for researching the expression of key genes involved in the host response to bacterial infection and sepsis by including genes related to IL-1R and TLR genes involved in the detection of pathogens.
· Click here for gene information |
| Interferon α, β Response and Regulators PCR Library |
Interferon α, β Response and Regulators PCR Library is for researching the expression of key genes involved in the interferon-α and interferon-β immune responses.
· Click here for gene information |
| Leukocyte Migration PCR Library |
Leukocyte Migration PCR Library is for researching the expression of key genes involved in functions, such as cytoskeletal remodeling, cell-cell interaction/adherence, and intracellular transduction.
· Click here for gene information |
| Phagocytosis PCR Library |
Phagocytosis PCR Library is for researching the expression of key genes involved in phagocyte-microbe contaction, intracellular signals, cytoskeletal rearrangement, alterations in membrane trafficking, activation of microbial killing mechanisms, production of pro- and anti-inflammatory cytokines and chemokines, activation of apoptosis, and production of molecules required for efficient antigen presentation to the adaptive immune system.
· Click here for gene information |
| T Cell Activation and Regulators Pathway PCR Library |
T Cell Activation and Regulators Pathway PCR Library are for researching the expression of key genes representing T cell activation, a key part of adaptive immunity.
· Click here for gene information |
| T Cell Tolerance and Anergy PCR Library |
T Cell Tolerance and Anergy PCR Library is for researching the expression of key genes involved in T-cell anergy and immune tolerance by including cell and B-cell regulators, cytokines and receptors, TNF superfamily members and their receptors, and transcriptional factors.
· Click here for gene information |
| T helper 17 Cells and Regulators PCR Library |
T Helper 17 Cells and Regulators PCR Library is for researching the expression of key genes involved in the functioning and characterization of Th17 cells, such as Th17- specific chemokines/cytokines and their receptors, important signaling molecules, genes induced by IL17, key surface markers and transcription factors that lead to Th17 development.
· Click here for gene information |
| Toll-like Receptor Pathway PCR Library |
Toll-like Receptor Pathway PCR Library is for researching the expression of key genes which are important to TLR-mediated signal transduction and innate immunity mediated by pattern recognition receptors (PRRs).
· Click here for gene information |
| Amino Acid Metabolism PCR Library |
Amino Acid Metabolism PCR Library is for researching the expression of key genes involved in biosynthesis and degradation of functional amino acids.
· Click here for gene information |
| Fatty Acid Metabolism PCR Library |
Fatty Acid Metabolism PCR Library is for researching the expression of key genes involved in the regulation and enzymatic pathways of fatty acid metabolism which is the process of energy generation and storing. Activated fatty acid is produced by breaking down of triacylglycerols, then it is transported into the mitochondria, where acetyl-CoA is obtained by α-oxidation. During resting states, cells store excess energy by re-systhesizing fatty acids.
· Click here for gene information |
| Glucose Metabolism PCR Library |
Glucose Metabolism PCR Library is for researching the expression of key genes involved in the regulation and enzymatic pathways of glucose and glycogen metabolism.
· Click here for gene information |
| Oxidation and Regulation PCR Library |
Oxidation and Regulation PCR Library is for researching the expression of key genes involved in oxidative stress including glutathione peroxidases (GPx), peroxiredoxins (TPx). reactive oxygen species (ROS) metabolism factors, oxidative stress responsive genes and genes involved in superoxide metabolism such as superoxide dismutases (SOD).
· Click here for gene information |
| 14-3-3 Induced Intracellular Signaling Pathway PCR Library |
14-3-3 Induced Intracellular Signaling Pathway PCR Library contains genes associated with 14-3-3 induced intracellular signaling. The 14-3-3 proteins are a family of conserved adaptor and scaffolding proteins. The 14-3-3 proteins are able to bind a multitude of functionally wide array of cellular proteins, including kinases, phosphatases and transmembrane receptors.
· Click here for gene information |
| ABC Transporters PCR Library |
ABC (ATP-binding cassette) Transporters PCR Library contains genes associated with superfamily of membrane proteins that translocate a variety of substrates across intra- and extra-cellular membranes. ABC transporters related diseases include cystic fibrosis, type II diabetes, cholesterol and bile transport defects, drug response phenotypes, anemia, retinal degeneration, and neurological disease. ABC transporters play important roles in absorption, distribution, metabolism, excretion and toxicology.
· Click here for gene information |
| AHR Signaling Pathway PCR Library |
AHR Signaling Pathway PCR Library contains genes associated with the bHLH (basic Helix-Loop-Helix)- PAS (PerARNT-Sim) family of transcriptional regulators that control a variety of developmental and physio- logical events, including neurogenesis, tracheal and Salivary duct formation, toxin metabolism, circadian rhythms, response to hypoxia and hormone receptor function.
· Click here for gene information |
| AMPK Signaling Pathway PCR Library |
AMPK Signaling Pathway PCR Library is for researching the expression of key genes involved in AMPK signaling which is a 3-subunit protein that acts as a master switch for energy sensing and homeostasis during many metabolic processes. It includes glucose uptake, β-oxidation of fatty acids and modulation of insulin secretion that occurs as a result of nutrient and stress signals.
· Click here for gene information |
| Androgens Signaling Pathway PCR Library |
Androgens Signaling Pathway PCR Library is for researching the expression of key genes involved in androgen synthesis including regulators of androgen production, receptors and signal transduction proteins which mediate their effects, and targets of androgen signaling.
· Click here for gene information |
| BMP Signaling Pathway PCR Library |
BMP Signaling Pathway PCR Library contains genes associated with BMPs (Bone Morphogenetic Proteins) which are a large subclass (more than 20 members) of the TGF-Beta (Transforming Growth Factor-Beta) super-family. These are active in many tissues under normal physiological conditions and are regulated through reversible interactions with extracellular antagonists, including noggin, chordin, follistatin, and DAN.
· Click here for gene information |
| BRCA1 Signaling Pathway PCR Library |
BRCA1 Signaling Pathway PCR Library contains genes associated with BRCA1 pathways. The maintenance of genome integrity is essential for all life, but is particularly important to long-lived multicellular organisms, which are susceptible to cancer. BRCA1 is keys for recognizing and repairing DNA damage.
· Click here for gene information |
| cAMP Signaling Pathway PCR Library |
cAMP Signaling Pathway PCR Library contains genes that are responsive to cAMP or calcium ion (Ca2+). This Library also contains genes whose promoters contain SRE or SRE-like enhancer sequences, the CRE enhancer sequence, and other Ca2+ responsive elements.
· Click here for gene information |
| Cell Death Signaling Pathway PCR Library |
Cell Death Signaling Pathway PCR Library contains key genes that are important for the central mechanisms of cellular death, such as apoptosis, autophagy, and necrosis.
· Click here for gene information |
| CMV and MAPK Signaling Pathways PCR Library |
CMV and MAPK Signaling Pathways PCR Library is for researching the expression of key genes involved in CMV and MAPK signaling pathway which is important for cell processes, such as growth, differentiation, and proliferation..
· Click here for gene information |
| CREB Signaling Pathway PCR Library |
CREB Signaling Pathway PCR Library is for researching the expression of key genes involved in CREB signaling pathway which is important in the process of consolidating a new memory and the dynamic complexity of information. These processes within neuronal networks are greatly increased by activity-dependent changes in gene expression within individual neurons.
· Click here for gene information |
| DNA Damage Signaling Pathway PCR Library |
DNA Damage Signaling Pathway PCR Library is for researching the expression of key genes involved in DNA damage signaling pathways.
· Click here for gene information |
| EGF / PDGF Signaling Pathway PCR Library |
EGF / PDGF Signaling Pathway PCR Library is for researching the expression of key genes involved in EGF(Epidermal Growth Factor) / PDGF(Platelet-Derived Growth Factor)-mediated signal transduction pathways.
· Click here for gene information |
| eNOS Signaling Pathway PCR Library |
eNOS Signaling Pathway PCR Library is for researching the expression of key genes involved in NO (Nitric Oxide) signaling pathway including a short-lived free radical gas related with diverse physiological and pathological processes.
· Click here for gene information |
| Erythropoietin Signaling Pathway PCR Library |
Erythropoietin Signaling Pathway PCR Library is for researching the expression of key genes involved in erythropoiesis which is a major pathway by which a pluripotent hematopoietic stem cell gives rise to mature end stage cells.
· Click here for gene information |
| Estrogens Signaling Pathway PCR Library |
Estrogens Signaling Pathway PCR Library is for researching the expression of key genes related in breast cancer regulation and estrogen receptor-dependent signal transduction including the enzymes involved in synthesis of estrogens and the estrogen receptors whose expression mediates the effects of estrogens.
· Click here for gene information |
| FAS Signaling Pathway PCR Library |
FAS Signaling Pathway PCR Library is for researching the expression of key genes involved in Fas signaling pathway which is related to a death domain-containing member of the TNFR (Tumor Necrosis Factor Receptor) superfamily. It has a central role in the physiological regulation of programmed cell death and has been implicated in the pathogenesis of various malignancies and diseases of the immune system.
· Click here for gene information |
| FGF Signaling Pathway PCR Library |
FGF Signaling Pathway PCR Library is for researching the expression of key genes involved in angiogenesis which is plays a key role in many physiological and pathological processes, such as ovulation, embryogenesis, wound repair, inflammation, malignant tumor growth, retinopathies, rheumatoid arthritis, and angiogenesis-dependent diseases.
· Click here for gene information |
| GAP Junctions Signaling Pathway PCR Library |
GAP Junctions Signaling Pathway PCR Library is for researching the expression of key genes involved in Gap junction channel which is spanned between two plasma membranes and is formed by the alignment of two hemichannels with oligomerized structural subunit proteins, called Cxs (Connexins).
· Click here for gene information |
| G-protein coupled receptor Signaling Pathway PCR Library |
G-Protein Coupled Receptor Signaling Pathway PCR Library is for researching the expression of key genes encoding the most important G-Protein Coupled Receptors (GPCR) which regulate a number of normal biological processes and play roles in the pathophysiology of many diseases upon dysregulation of their downstream signal transduction activities.
· Click here for gene information |
| Growth Factors Signaling Pathway PCR Library |
Growth Factors Signaling Pathway PCR Library is for researching the expression of key genes related to growth factors which play a vital role in various normal biological processes, such as embryogenesis, wound healing and inflammation. This array contains angiogenic growth factors and regulators of apoptosis.
· Click here for gene information |
| GTPase Signaling Pathway PCR Library |
GTPase Signaling Pathway PCR Library is for researching the expression of key genes encoding the small GTP-binding proteins which comprise a group of signaling molecules. These are activated a variety of growth factors, cytokines, adhesion molecules, hormones, integrins, G-proteins and other biologically active substances. They regulate a wide range of biological processes, including reorganization of the actin cytoskeleton, transcriptional regulation, vesicle trafficking, morphogenesis, neutrophil activation, phagocytosis, activation of the NADPH oxidase, mitogenesis, apoptosis and tumorigenesis.
· Click here for gene information |
| Hedgehog Signaling Pathway PCR Library |
Hedgehog Signaling Pathway PCR Library is for researching the expression of key genes involved in the hedgehog signaling pathway which is the most well-known morphogens involved in the developmental pattern formation of various organs, such as the nervous system, muscle, the heart and the lungs.
· Click here for gene information |
| HOX Gene PCR Library |
HOX Gene PCR Library is for researching the expression of key genes involved in multicellular organismal development, such as a group of homeodomain-containing transcription factors.
· Click here for gene information |
| Hypoxia Signaling Pathway PCR Library |
Hypoxia Signaling Pathway PCR Library is for researching the expression of key genes associated with hypoxia-related signaling which is directly involved in the response to hypoxia and oxidative stress.
· Click here for gene information |
| IGF1R Signaling Pathway PCR Library |
IGF1R Signaling Pathway PCR Library is for researching the expression of key genes involved in IGF1R signaling which is a genetically controlled means of cellular self-destruction leading to dismantling and packaging of cell material for removal by phagocytosis.
· Click here for gene information |
| ILK Signaling Pathway PCR Library |
ILK Signaling Pathway PCR Library is for researching the expression of key genes associated with ILK signaling, which is involved in cell adhesion, filament rearrangement and cell migration.
· Click here for gene information |
| iNOS Signaling Pathway PCR Library |
iNOS Signaling Pathway PCR Library is for researching the expression of key genes involved in iNOS signaling.
· Click here for gene information |
| Insulin Signaling Pathway PCR Library |
Insulin Signaling Pathway PCR Library is for researching the expression of key genes involved in insulin-responsive and signaling pathway.
· Click here for gene information |
| Intracellular Calcium Signaling Pathway PCR Library |
Intracellular Calcium Signaling Pathway PCR Library is for researching the expression of key genes involved in cellular activities in virtually all cell types that are regulated by common intracellular signaling systems. The calcium is one of important ubiquitous intracellular messenger, it controls diverse range of cellular processes, such as gene transcription, muscle contraction and cell proliferation.
· Click here for gene information |
| JAK / STAT Signaling Pathway PCR Library |
JAK / STAT Signaling Pathway PCR Library is for researching the expression of key genes involved in JAK- and STAT-mediated signaling, receptors that activate them, nuclear co-factors and co-activators associated with the STAT proteins, STAT-inducible genes and negative regulators of the pathway.
· Click here for gene information |
| LDL Signaling Pathway PCR Libraryy |
LDL Signaling Pathway PCR Library is for researching the expression of key genes involved in LDL (Low Density Lipoproteins) pathway which is the major carriers of cholesterol in humans. This pathway plays an important role in lipoprotein metabolism and in cellular cholesterol homeostasis.
· Click here for gene information |
| Lipoprotein Signaling and Cholesterol Metabolism Pathway PCR Library |
Lipoprotein Signaling and Cholesterol Metabolism Pathway PCR Library is for researching the expression of key genes involved in lipoprotein transport and cholesterol metabolism.
· Click here for gene information |
| MAP Kinase Signaling Pathway PCR Library |
MAP Kinase Signaling Pathway PCR Library is for researching the expression of key genes involved in the MAP kinase (MAPK) signaling pathway.
· Click here for gene information |
| Melatonin Signaling Pathway PCR Library |
Melatonin Signaling Pathway PCR Library is for researching the expression of key genes involved in melatonin signaling by secreted hormone mainly from the pineal gland or epiphysis and retina.
· Click here for gene information |
| mTOR Signaling Pathway PCR Library |
mTOR Signaling Pathway PCR Library is for researching the expression of key genes involved in the mammalian target of rapamycin (mTOR) signaling pathway which integrates responses from a wide variety of signals (nutrients, hormones, growth factors and cellular stresses) to regulate cell growth, metabolism and survival.
· Click here for gene information |
| Neurotransmitter Receptors and Regulators PCR Library |
Neurotransmitter Receptors and Regulators PCR Library is for researching the expression of key genes involved in modulating the biological processes of neurotransmitter biosynthesis, uptake, transport and signaling through neurotransmitter receptors.
· Click here for gene information |
| Neurotrophins and Receptors PCR Library |
Neurotrophins and Receptors PCR Library is for researching the expression of key genes involved in neuronal processes which include neurotrophins and neuropeptides along with their receptors.
· Click here for gene information |
| NF-kB Signaling Pathway PCR Library |
NF-kB Signaling Pathway PCR Library is for researching the expression of key genes involved in NF-κB-mediated signal transduction which mediates the regulation of viral replication, autoimmune diseases, inflammatory response, tumorigenesis and apoptosis.
· Click here for gene information |
| NGF Signaling Pathway PCR Library |
NGF Pathway Signaling PCR Library is for researching the expression of key genes involved in NGF signaling patyway which has the effects on neuronal growth and survival. These are mediated by a high affinity cell surface receptor TRKA (Tyrosine Kinase Receptor-A) and a low affinity neurotrophic receptor, p75(NTR) (p75 Neurotrophic Receptor).
· Click here for gene information |
| nNOS Signaling Pathway PCR Library |
nNOS Signaling PCR Library is for researching the expression of key genes involved in nNOS signaling pathway.
· Click here for gene information |
| NO(Nitric Oxide) Signaling Pathway PCR Library |
NO (Nitric Oxide) Signaling Pathway PCR Library is for researching the expression of key genes whose expression is controlled by or involved in signaling by the second messenger nitric oxide (NO) and contained genes related with nitric oxide biosynthesis, superoxide metabolism and response to oxidative stress.
· Click here for gene information |
| Notch Signaling Pathway PCR Library |
Notch Signaling Pathway PCR Library is for researching the expression of key genes involved in Notch signaling which has many binding and receptor processing molecules. This signaling is related to apoptosis, cell cycle, neurogenesis and the regulation of transcription as well as genes involved in cell regulation, proliferation, and differentiation.
· Click here for gene information |
| Nuclear Receptors, Coregulators and Regulator Pathway PCR Library |
Nuclear Receptors and Coregulators Pathway PCR Library is for researching the expression of key genes encoding nuclear receptors and their coregulators.
· Click here for gene information |
| p53 Signaling Pathway PCR Library |
p53 Signaling Pathway PCR Library is for researching the expression of key genes related to p53-mediated signal transduction. This signal transduction involves the processes of apoptosis, cell cycle, cell growth, proliferation, differentiation and DNA repair.
· Click here for gene information |
| PDGF Signaling Pathway PCR Library |
PDGF Signaling Pathway PCR Library is for researching the expression of key genes involved in PDGF signaling pathway which is a member of a large family of growth factors including VEGF (Vascular Endothelial Growth Factor), PlGF (Placental Growth Factor), CTGF (Connective Tissue Growth Factor) and PDGF-like factor secreted by human vascular endothelial cells and fibroblasts.
· Click here for gene information |
| PI3K-AKT Signaling Pathway PCR Library |
PI3K-AKT Signaling Pathway PCR Library is for researching the expression of key genes involved in PI3K-AKT signaling which includes inactivation of Gsk3, the accumulation of β-catenin, regulation of actin organization, cell migration and BAD phosphorylation.
· Click here for gene information |
| PKA Signaling Pathway PCR Library |
PKA Signaling Pathway PCR Library is for researching the expression of key genes involved in PKA signaling pathway which regulates the processes as diverse as growth, development, memory and metabolism.
· Click here for gene information |
| PPAR Signaling Pathway PCR Library |
PPAR Signaling Pathway PCR Library is for researching the expression of key genes encoding Peroxisome Proliferator-Activated Receptors (PPAR) which are inducible transcription factors belonging to a superfamily of nuclear hormone receptors. Gene expression profile with this Library can provide information associated with a wide variety of functions, such as fatty-acid oxidation, synthesis of cholesterol, steroids and other lipids.
· Click here for gene information |
| Protein Kinase Pathways PCR Library |
Protein Kinase Pathways PCR Library is for researching the expression of key genes involved in protein kinase pathway which has the ability to reversibly phosphorylate proteins, which in turn regulates their function.
· Click here for gene information |
| Protein Phosphatases PCR Library |
Protein Phosphatases PCR Library is for researching the expression of key genes encoding important phosphatases which is key regulatory proteins mediated by protein kinases. And it serves as a very important complement to kinases, and also, attenuates activated signal transduction pathways.
· Click here for gene information |
| RAS Signaling Pathway PCR Library |
RAS Signaling Pathway PCR Library is for researching the expression of key genes involved in Ras signaling which is normally activated in response to the binding of extracellular signals, such as growth factors, RTKs (Receptor Tyrosine Kinases), TCR (T-Cell Receptors) and PMA (Phorbol-12 Myristate-13 Acetate).
· Click here for gene information |
| RHOA Signaling Pathway PCR Library |
RHOA Signaling Pathway PCR Library is for researching the expression of key genes encoding the Rho which is a member of the Ras superfamily of small GTP-binding proteins that play a central role in diverse biological processes, such as actin cytoskeleton organization, microtubule dynamics, gene transcription, oncogenic transformation, cell cycle progression, adhesion and epithelial wound repair.
· Click here for gene information |
| Signal Transduction Pathway PCR Library |
Signal Transduction Pathway PCR Library is for researching the expression of key genes involved in representative of signal transduction pathways.
· Click here for gene information |
| SMAD Signaling Pathway PCR Library |
SMAD Signaling Pathway PCR Library is for researching the expression of key genes involved in SMAD signaling pathway which is activated by TGF-Beta (Transforming Growth Factor-Beta) superfamily. This plays an important role in a variety of pathophysiologic processes including angiogenesis, vascular remodeling, atherogenesis and regulating cellular responses, such as growth, proliferation, differentiation, migration, adhesion, survival and specification of developmental fate.
· Click here for gene information |
| TGF-β Signaling Pathway PCR Library |
TGF-β Signaling Pathway PCR Library is for researching the expression of key genes responsive to TGF-β signal transduction which comprise TGF- β, Bone Morphogenic Protein (BMP), activin and nodal. These pathways regulate multiple cellular processes, such as development, differentiation, cellular proliferation and migration, apoptosis, and cell cycle regulation.
· Click here for gene information |
| Thrombin Signaling Pathway PCR Library |
Thrombin Signaling Pathway PCR Library is for researching the expression of key genes associated with thrombin signaling and candidates for endogenous control genes.
· Click here for gene information |
| TNFR Pathway PCR Library |
TNFR Pathway PCR Library is for researching the expression of key genes involved in TNFR signaling pathway which plays important roles in various physiological and pathological processes, including cell proliferation, differentiation, apoptosis, modulation of immune responses, and induction of inflammation.
· Click here for gene information |
| Transcription Factors (Non-HOX) PCR Library |
Transcription Factors (Non-HOX) PCR Library is for researching the expression of key genes encoding transcription factors containing one or more DNA binding domain(s) which binds to either enhancer or promoter regions of DNA adjacent to the genes that they regulate.
· Click here for gene information |
| Transcription of RNA PCR Library |
Transcription of RNA PCR Library is for researching the expression of key genes involved in transcription and transcriptional factors which do the process through DNA sequence that enzymatically copied by an RNA polymerase to produce a complementary RNA.
· Click here for gene information |
| TNFR Pathway PCR Library |
Tumor Necrosis Factor (TNF) Signaling Pathway PCR Library is for researching the expression of key genes whose expression is controlled by or involved in the TNF ligand and TNF receptor signaling pathways.
· Click here for gene information |
| VEGF Signaling Pathway PCR Library |
VEGF Signaling PCR Library is for researching the expression of key genes involved in signal transduction and cell signaling downstream of vascular endothelial growth factors (VEGF) and their receptors which is critical for regulating new blood vessel formation including angiogenesis, arteriogenesis or vasculogenesis.
· Click here for gene information |
| WNT Signaling Pathway PCR Library |
WNT Signaling Pathway PCR Library is for researching the expression of key genes involved in signal transduction which plays an important role in maintenance processes, such as homeostasis and cell cycle regulation.
· Click here for gene information |
| Embryonic stem cell PCR Library |
Embryonic Stem Cell PCR Library is for researching the expression of key genes involved in the maintenance of pluripotency and the self-renewal status of embryonic stem cells.
· Click here for gene information |
| Hematopoietic Stem cells(HSCs) PCR Library |
Hematopoietic Stem Cells (HSCs) PCR Library is for researching the expression of key genes involved in the development of blood-cell lineages from HSCs through progenitor stem cells.
· Click here for gene information |
| IPS PCR Library |
IPS(Induced Pluripotent Stem Cells) PCR Library is for researching the expression of key genes involved in pluripotency and early differentiation.
· Click here for gene information |
| Mesenchymal Stem Cells PCR Library |
Mesenchymal Stem Cells PCR Library is for researching the expression of key genes involved in maintaining pluripotency and self-renewal status which differentiates into a variety of cell types, such as osteoblasts, chondrocytes, myocytes, adipocytes and beta-pancreatic islets cells.
· Click here for gene information |
| Neuronal Stem cell PCR Library |
Neuronal Stem Cell PCR Library is for researching the expression of key genes involved in neurogenesis and neural stem cell differentiation which are related to the regulation of cell cycle, cell proliferation, differentiation, motility and migration.
· Click here for gene information |
| Stem cell PCR Library I, II, III |
Stem Cell PCR Library I, II, III are for researching the expression of key genes related to the identification, growth and differentiation of stem cells.
· (Stem Cell PCR Library I )Click here for gene information · (Stem Cell PCR Library II )Click here for gene information · (Stem Cell PCR Library III ) Click here for gene information |
| Cardiotoxicity PCR Library |
Cardiotoxicity PCR Library is for researching the expression of key genes involved in drug and chemical-induced cardiac injury.
· Click here for gene information |
| Drug Metabolism PCR Library |
Drug Metabolism PCR Library is for researching the expression of key genes critical in the metabolism of drugs, toxic chemicals, hormones and micronutrients which are important to pharmacology, endocrinology and food science.
· Click here for gene information |
| Drug Transportation PCR Library |
Drug Transportation PCR Library is for researching the expression of key genes encoding transporter proteins which play an important role in pharmacology, affecting entry and extrusion of drugs into and out of cells.
· Click here for gene information |
| Hepatotoxicity PCR Library |
Hepatotoxicity PCR Library is for researching the expression of key genes implicated as potential biomarkers of liver toxicity by including steatosis, phospholipidosis, non-genotoxic hepato-carcinogenicity and necrosis, as well as generalized hepatotoxicity biomarker.
· Click here for gene information |
| Nephrotoxicity PCR Library |
Nephrotoxicity PCR Library is for researching the expression of key genes implicated as potential biomarkers of kidney toxicity.
· Click here for gene information |
| Neurotoxicity PCR Library |
Neurotoxicity PCR Library is for researching the expression of key genes involved in drug and chemical-induced neurotoxic responses.
· Click here for gene information |
| Toxicology Pathway PCR Library |
Toxicology Pathway PCR Library is for researching the expression of key genes involved in at least 13 different biological pathways which are activated by response to toxic drugs. Profiling the gene expression level using this library could help to identify toxicological responses against treatment of some candidate drugs to human cell line.
· Click here for gene information |
| Biosynthesis PCR Library | |
| DNA & RNA Processing PCR Library | |
| Transport PCR Library | |
| Zinc Finger Proteins PCR Library |
| Unclassified Genes |
| Affiliation / Research topics |
| Department of Molecular Dermatology, Kyung Hee University / Gene Expression by Treatment of Whitening Substance |
 Reviews:Overall, I am satisfied with your service. I liked how the person in charge could provide us detailed information both before and after the service. |
| Department of Plastic and Reconstructive Surgery, Myongji Hospital / Infection control and tissue proliferation by specific substances |
| Reviews: I was having a difficult time to communicate with my fellow school researchers about my qPCR project. It wasn’t going well, but Bioneer helped us by fulfilling our needs and undergoing the service as we wanted. From this, we realized how experienced this institute was. |
| Medicine Cancer Institute of Seoul National University / Signaling pathway in gastric cancer cells |
| Reviews: We think that Bioneer's qPCR array is very attractive in that it is possible to make an optional request for sample preparation before array, and to customize the gene to be analyzed without shifting it to the signaling pathway. It is a big advantage that the screening can be done in a short time because the difference in expression is calculated using the statistical value in about one week after the sample is submitted. You can also analyze the difference between the samples by giving raw data like Ct value to the result file. I think this service can provide researchers a lot of data in a short time. |
| Medicine Cancer Institute of Seoul National University / Gene expression by cytokine treatment of oncogene overexpressing cells |
| Reviews: For these kinds of service, I believe the most important aspects are not only how inovative the technology is, but also how cooperative the service provider is. qPCR array team did a great job explaining all my queries and constantly giving us feedbacks about its progress. Moreover, parts of the results were separately done by us using qPCR, and surprisingly, it was similar to the ones I got from Bioneer. |
| Department of Oral Pathology, College of Dentistry, Chosun University / Gene expression between wild cells and anticancer-resistant cells in oral cancer cells |
| Reviews: When we checked the results again in our laboratory, I like how reproducible those were. |
| Physiology classroom of Cha University / Src family kinase anti-cancer effect |
| Reviews: In order to clarify the relationship between the gene expression pattern and the anticancer effect of src family kinase in pancreatic cancer, mRNA expression patterns of pancreatic cancer cell line MIA PaCa and Capan src family gene were requested. We were very satisfied how the results were similar to the ones we got from our laboratory. We believe we will use more services provided by Bioneer as we continue our researches. Thank you for providing us with well-organized data. |
| Korea Research Institute of Bioscience and Biotechnology Protein Research Center / Cancer related NF-kB signaling |
| Reviews: I explained everything from the design stage to the result, and I was satisfied with the service. Bioneer clearly explained to us everything from the designing of protocols to gaining of results. Overall, I am satisfied with the service. |
| Korea Research Institute of Bioscience and Biotechnology Chemical Biology Research Center / Regulation of central gene related to welfare genetics |
| Reviews: Overall, I felt satisfied with the results. |
* Above contents are some of reviews gained from our past customers with their consents.
Beside those institutes, we have provided our service to Gangnam Cha Hospital CHA Cancer Institute, College of Pharmacy Kunkuk University, Department of Microbiology, College of Medicine, Gyeongsang National University, Department of Life Science, Catholic University of Daegu, Life Science Research Institute of Asan Medical Center, Department of Radiology of Asan Medical Center, Sookmyung Women's University Immunology Laboratory, Department of Internal Medicine, Yonsei University College of Medicine, Korea Institute of Oriental Medicine etc.
▶ List of articles using bioneer's qPCR array service
| Publication year | Title | Author | Journal |
| 2021 | Sex-related Differences in Glial Fibrillary Acidic Protein-positive GABA Regulate Neuropathology Following Pilocarpine-induced Status Epilepticus. | Hong, G. P., Kim, M. H., & Kim, H. J. | Neuroscience, 472, 157-166. |
| 2021 | Mitochondrial-encoded MOTS-c prevents pancreatic islet destruction in autoimmune diabetes | Kong, B. S., Min, S. H., Lee, C., & Cho, Y. M. | Cell Reports, 36(4), 109447. |
| 2021 | O-Linked N-Acetylglucosamine Modification of Mitochondrial Antiviral Signaling Protein Regulates Antiviral Signaling by Modulating Its Activity. | Seo, J., Park, Y. S., Kweon, T. H., Kang, J., Son, S., Kim, H. B., ... & Cho, J. W. | Frontiers in immunology, 11, 3681. |
| 2020 | Particulate matter less than 10 μm (PM10) activates cancer related genes in lung epithelial cells. | Kang, D., Jung, I. B., Lee, S. Y., Park, S. J., Kwon, S. J., Park, D. H., & Son, J. W. | Inhalation Toxicology, 32(13-14), 487-493. |
| 2020 | Genome based quantification of VHSV in multiple organs of infected olive flounder (Paralichthys olivaceus) using real-time PCR. | Kim, H., Baek, K. W., Ko, E. J., Luan, N. T., Lim, Y., Roh, H. J., ... & Cha, H. J. | Genes & Genomics, 42, 773-777. |
| 2018 | Additive effect of oral LDD175 to tamsulosin and finasteride in a benign prostate hyperplasia rat model. | Choi, B. R., Kim, H. K., Soni, K. K., Karna, K. K., Lee, S. W., So, I., & Park, J. K. | Drug design, development and therapy, 12, 1855. |
| 2018 | VEGF siRNA delivery by a cancer-specific cell-penetrating peptide. | Lee, Y. W., Hwang, Y. E., Lee, J. Y., Sohn, J. H., Sung, B. H., & Kim, S. C. | Journal of microbiology and biotechnology, 28(3), 367-374. |
| 2018 | Additive effect of oral LDD175 to tamsulosin and finasteride in a benign prostate hyperplasia rat model. | Choi, B. R., Kim, H. K., Soni, K. K., Karna, K. K., Lee, S. W., So, I., & Park, J. K. | Drug design, development and therapy, 12, 1855. |
| 2017 | Montanide IMS 1312 VG adjuvant enhances the efficacy of immersion vaccine of inactivated viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus. | Hwang, J. Y., Kwon, M. G., Kim, Y. J., Jung, S. H., Park, M. A., & Son, M. H. | Fish & shellfish immunology, 60, 420-425. |
| 2016 | Set7/9, a methyltransferase, regulates the thermogenic program during brown adipocyte differentiation through the modulation of p53 acetylation. | Son, M. J., Kim, W. K., Park, A., Oh, K. J., Kim, J. H., Han, B. S., ... & Bae, K. H. | Molecular and cellular endocrinology, 431, 46-53. |
| 2016 | FOXC2 and CLIP4: a potential biomarker for synchronous metastasis of≤ 7-cm clear cell renal cell carcinomas. | Ahn, J., Han, K. S., Heo, J. H., Bang, D., Kang, Y. H., Jin, H. A., ... & Ham, W. S. | Oncotarget, 7(32), 51423. |
| 2015 | Negative pressure wound therapy of chronically infected wounds using 1% acetic acid irrigation. | Jeong, H. S., Lee, B. H., Lee, H. K., Kim, H. S., Moon, M. S., & Suh, I. S. | Archives of plastic surgery, 42(1), 59. |
| 2015 | Influence of gastrointestinal stress on autoinducer-2 activity of two Lactobacillus species. | Yeo, S., Park, H., Ji, Y., Park, S., Yang, J., Lee, J., ... & Holzapfel, W. | FEMS microbiology ecology, 91(7). |
| 2015 | Intracellular annexin A2 regulates NF-κ B signaling by binding to the p50 subunit: implications for gemcitabine resistance in pancreatic cancer. | Jung, H., Kim, J. S., Kim, W. K., Oh, K. J., Kim, J. M., Lee, H. J., ... & Bae, K. H. | Cell death & disease, 6(1), e1606-e1606. |
| 2014 | Ectopic expression of CaWRKY1, a pepper transcription factor, enhances drought tolerance in transgenic potato plants. | Moon, S. J., Han, S. Y., Kim, D. Y., Kim, B. G., Yoon, I. S., Shin, D., ... & Byun, M. O. | Journal of Plant Biology, 57(3), 198-207. |
| 2014 | Autoinducer-2 associated inhibition by Lactobacillus sakei NR28 reduces virulence of enterohaemorrhagic Escherichia coli O157: H7. | Park, H., Yeo, S., Ji, Y., Lee, J., Yang, J., Park, S., ... & Holzapfel, W. | Food Control, 45, 62-69. |
| 2013 | Abrogation of galectin-4 expression promotes tumorigenesis in colorectal cancer. | Kim, S. W., Park, K. C., Jeon, S. M., Ohn, T. B., Kim, T. I., Kim, W. H., & Cheon, J. H. | Cellular Oncology, 36(2), 169-178. |
| 2013 | Cholesterol induces cardiac hypertrophy by activating the AKT pathway. | Lee, H., Yoo, Y. S., Lee, D., & Song, E. J. | The Journal of steroid biochemistry and molecular biology, 138, 307-313. |
| 2013 | Cancer upregulated gene 2, a novel oncogene, confers resistance to oncolytic vesicular stomatitis virus through STAT1-OASL2 signaling. | Malilas, W., Koh, S. S., Srisuttee, R., Boonying, W., Cho, I. R., Jeong, C. S., ... & Chung, Y. H | Cancer gene therapy, 20(2), 125-132. |
| 2012 | Indirubin-3-monoxime exhibits anti-inflammatory properties by down-regulating NF-κB and JNK signaling pathways in lipopolysaccharide-treated RAW264. 7 cells. | Kim, J. K., & Park, G. M. | Inflammation Research, 61(4), 319-325. |
