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Gene synthesis service of the first and the largest scale in KOREA
Our products guarantee 100% accuracy of your ordered DNA or protein sequences with minimized time. Bioneer utilizes its independently developed "HT-oligo™ Synthesizer" for the most advanced technology to synthesize an oligo. From 1995, our company has started gene synthesis for the first time in Korea and supplied countless amounts of synthetics genes. With our continuous endeavor and long experience to reduce the delivery time and cost, we are always trying our best to provide the best service as possible
• Accuracy
Guaranteed exact plasmid DNA sequences with Bioneer's Gene synthesis service
• Rapid
Gene synthesis avaliable only within minimum of 5 days
• AccuGeneBlock Service
Quick and easy service providing DNA fragments of 1 Kbp or less for synthetic biology research
Gene Synthesis
Rapid Gene Synthesis
AccuGeneBlock
Delivery Form
Plasmid DNA
Plasmid DNA
DNA Fragmant (PCR product)
Gene length
1 bp ~
1 ~ 1000 bp
100 ~ 1000 bp
Turnaround Time
5 ~ working days
Guaranteed 5, 8 working days
5 ~ 6 working days
Features and Benefits
Rapid and economical
Fast and reasonable price by constructing integrated production system from raw materials (phosphoramidite, reagents and solvents) to high-speed oligo synthesizer
Accurate quality
Guaranteed 100% sequence using Automatic DNA sequencer (ABI 3730)
Codon optimization service
Accumulated experience and techniques for optimization of protein expression and codon
Comprehensive vector choice
Wide range of choices for compatible vectors according to the desired applications at a reasonable price and time
* By using the world's first protein automatic synthesizer ExiProgen™ developed by Bioneer, maximum of 16 proteins can be obtained with fully automated protocols only within six hours from synthetic genes
Vectors can be chosen when ordering the gene synthesis or cloning service, allowing to save money by eliminating the need of buying commercial vectors and to easily get the plasmid of your desired form.
By selecting the pBT7-N-His and pBT7-C-His vector having the best compatibility with the Cell Free Protein Expression System, Bioneer’s automatic device ExiProgen™ or manual type kit AccuRapid can be used for rapid protein synthesis and purification with ease.
1 ~ 2 µg of vacuum dried plasmid *Low copy plasmid is 0.5 ~ 1 µg
Cloning Vector
pBHA
Basic vector
Other (Bioneer vector)
$50.00
Subcloning Price
$150.00 (If ordered with commercial or custom vector of your choice)
Plasmid Increase Service
*Low copy plasmid (Inquire)
> 4 μg
> 100 μg
> 300 μg
> 500 μg
$23
$54
$154
Inquire
* Price and delivery period are raised for gene segments containing complex structures such as high or low GC content, repeat sequences or homopolymeric runs * If you want to use subcloning with commercial vector, your order will be continued to Gene Cloning Service. * Only the synthesized genes with 100% sequence match will be shipped after the sequencing procedure * Genes that produces toxic substances or inhibits growth in E.coli cell system will be delivered in the form of PCR products or plasmid DNA, even during the sequence check during the synthesis process. * If the gene synthesis is ordered for the protein expression or purification, Bioneer's ExiProgen™ service can be used to directly obtain the proteins. (Go to ExiProgen™ page for more details )
* Order Cancellation Policy: 50% of the total charge will be billed if the service is cancelled within 5 days
80% of the total charge will be billed if the service is cancelled after 5 days No charges for refunds after the proposed period (excluding delivery time). It does not apply to 'Difficult Gene'
AccuGeneBlock Service
Synthetic price
100~500 bp
$95.00
501~1000 bp
$95.00 ( + $29.00/100 bp)
Synthesis period
100~500 bp
5 working days
501~1000 bp
6 working days
Delivery Form
500 ng ~ 1 µg of lyophilized PCR product
Additional Service
Increased Quantity
5 µg / $76.00
* Synthesis may be limited if the gene contains high or low GCs, repeat sequences, or homo-polymeric runs. (Please use Gene synthesis service) * 100bp~1000bp genes can be synthesized. * PCR products are provided with 3'A tailing, but blunt ends can be also selected.
Rapid Gene Synthesis Service
Synthesis period
1~500 bp
5 working days
501~1000 bp
8 working days
Synthetic price
Inquire
Delivery Form
1 ~ 2 µg of vacuum dried plasmid
Cloning Vector
pBHA (Basic vector)
Plasmid Increase Service
> 4 μg
> 100 μg
> 300 μg
> 500 μg
$23
$54
$154
Inquire
* Synthesis containing complex structures such as high or low GC, repeated sequences, homo-polymeric runs, etc. may be limited. (General Gene Synthesis service available) * Genes synthesis that inhibit growth or contain toxicity to E. coli cell system may be limited. (General Gene Synthesis service available), We do not guarantee the synthesis period if confirmed during synthesis. * If the synthesis is completed after the guarantee period, 40% of discount will be applied to the estimated price.
▶ Consultation
• Consultation service : Tel. +82-42-930-8777(Customer Support Center) or +82-42-930-8793, 8515 (Synthetic Biology Team), Please email geneorder@bioneer.com
• Consultation time: Weekdays from 9:00 am to 6:00 pm (closed on weekends and holidays)
1. Open Bioneer homepage(www.bioneer.co.kr) and click on Gene Synthesis Service Page.
2. Log in.
3. Enter the necessary information and confirm the quotation.
4. Click Add to Cart button; then place your final order. Synthesis will start on the same day if the orders are placed before 16:00. Otherwise, it will start on the next day. This service is unavaliable during the holiday period.
There is no limit in length. However, genes that go over 15Kb will take a long time.
Ordering longer than 15,000 bp, please input your genes DNA sequence into our order form, and submit geneorder@bioneer.com
Ordered genes are provided in the form of 1 to 2 µg dried plasmid DNA cloned in the basic vector.
Please refer to the table below to see the price of gene synthesis having simple sequences.
The cost and delivery time will be extended for genes with structures complicated for synthesis such as having a high GC content ratio, or tandem repeat, inverted repeat, or homo-polymeric sequence.
Furthermore, synthesis of genes inhibiting growth or having toxicity to E. coli will increase the service price (by 20%) and time. The extended period is unable to be estimated.
Gene Synthesis Service
Gene Length (bp)
Synthetic price(Normal sequence)
Synthesis Time
400 bp
$180
Average 5~10 working days
400 ~ 1200 bp
$0.39/bp
Average 5~10 working days
1201 ~ 2000 bp
Average 10~17 working days
2001 ~ 3000 bp
Average 15~25 working days
3001 ~ 15000 bp
$0.40 ~/bp
Average 20~30 working days
150001 bp ~
$0.50 ~/bp
Inquire
The synthesis period shown in the quotation is the average synthesis period.
This service synthesizes oligonucleotides and utilizes staggered hybridization, ligation, and PCR reactions for gene synthesis. Having repetitive sequences makes the hybridization processes difficult, increasing the difficulty ot the synthesis.
In this case, additional oligonucleotide designs are required with further synthesis and tests. Thus, additonal fees will cost, greatly varying on the length and type of the repeat sequence.
Yes, it can be done through a seperate cloning service. While ordering this service, select "Gene synthesis & Cloning" and specify the what you need (such as providing vector samples to us, enzyme sites, etc.). After checking the price, you can finalize your orders.
Furthermore, we offer 50% discount if the cloning service is ordered with the gene synthesis service.
Normally, the plasmids should last a year at -20℃.
Storage Condition
Shelf Life (Month)
at RT in water
2
at 4℃ in water
9
at -20℃ in water
18
at -20℃ (Dry)
24
▶ AccuGeneBlock synthesis
This service is only capable of synthesizing linear double-stranded DNA with a length of 1 kb or less without high GC conents or any tandem repeats, inverted repeats, or homo-polymeric sequences.
If your genes have features that inhibit the synthesis such as high & low GC, repeats, or inverted sequences, please use Gene Synthesis service.
Please use the gene synthesis service instead if you cannot please the order because of the above reason.
If you can not place your order for the above reason, please use Gene synthesis service instead of AccuGeneBlock service.
▶ Codon optimization
Yes, we provide the best codon optimization using our internal software. If you want codon optimization, please fill in the comments section when ordering.
▶ Risk sequence
Synthetic genes may cause severe issues if used with malicious intents. We try our best to prevent such kind of abuse through meticulous screening to stop distribution of harmful genes having potential to threat human health, agriculture, and livestock industries (Nature Biotechnology, 2009. Vol. 27(9). 800-081). To this end, we are also participating in the Harmonized screening protocol of the International Gene Synthesis Consortium (IGSC).
We categorize those containing Select Agents and Toxin sequences described in the Harmonized screening protocol of the International Gene synthesis Consortium (IGSC) as "Risk sequences."
Yes you may. However, we only provide risk sequences to bona fide research agents with meticulous screening. Buyers must sign a written consent form to indicate that they agree to use our Risk sequences only for research purposes.
▶ Ordering and others
All services are available online on our website.
After selecting the desired services and writing the required information, you may check the quotation to finalize your orders. Order will be confirmed once your payment is complete.
We do have a policy for a mass synthesis order. Please contact us separately.
If you want to change the sequence:
Sequence Change Length
Price
Both ends (5` / 3`) 9bp or less
$50.00/end
Within gene 20bp or less
$150.00(point mutation price applied)
Order cancellation period
Cancel Price
Within 5 days after ordering
50% of the total price
After 5 days of order
80% of total price
After guarantee period 1) Proposed period: Date for finalized gene synthesis except for delivery time. 2) This offer does NOT apply to ‘Difficult gene’.
Free
Yes, both are possible. However, while we offer directional cloning service for free, the plasmid increase service will incur additional fees and time.
Add 20ul (Final 250ng / ul) of DW or TE buffer to the delivered DNA. (It is recommended to add DW or TE buffer to tubes and store at 4℃ for 10 minutes.)
For the long-term storage, refrigerate it at -20°C. However, you can store at the room temperature if the DNA is in dried state.
We safely store your ordered genetic information along with the finished products for six months. Contact us to immediately discard them after the synthesis.