ExiPrep™ 16 Plus

ExiPrep™ 16 Plus extracts nucleic acids and purifies protein automatically up to 16 samples. Through optimized experimental protocols, it not only allows to get reproducible results, but also wide compatibility with various types of nucleic acids and protein samples. Furthermore, this instrument has excellent space utilization thanks to its small size. 

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SKU
A-5030

Features and Benefits

  • Built-in optimized nucleic acid extraction protocol

    Simplified usage and reproducible results with built-in nucleic acid extraction and protein purification protocols optimized for various samples and targets

  • High-purity nucleic acid extraction

    Extraction of highly pure nucleic acids by attaching magnetic particles to the bottom of each well equally and rapidly by magnetic blocks attached to the instrument
    Increased elution efficiency allowing complete evaporation of alcohols by its heating block

  • Prevention of contamination

    Substantial blocking of cross-contamination, which may occur in other wells by the aerosols generated from the tip, through the sophisticated designs drop-contamination prevention device located at the bottom of the filter tip

  • Automatic UV sterilization

    Sterilization to prevent infection for the users by the samples through the UV lamp installed inside the instrument

  • Easy-to-use

    User-friendly UI capable of checking the experimental progress rate real-time without the need of seperate PC by equipping with a 13.3-inch touch screen on the front of the device

  • Excellent Precision

    Minimized error range, that may occur during the automatic dispensing of the solution, through installment of an adjustable syringe motor up to 0.1 mm capable of stably distributing in a volume of 25 ㎕ ~ 1000 ㎕ for application to various protocols and obtaining of experimental results

Application

Division Application
University and Business Institute Nucleic acid extraction and protein purification from various samples
University Hospital Research Institute Nucleic acid extraction for research and development from blood, tissues and various biological samples
Food companies, restaurants Nucleic acid extraction required for GMO/food poisoning test in a short time from various foodstuffs
Military, police Nucleic acid extraction necessary for biologic terrorism causative bacteria and food poisoning causative bacteria

Workflow

ExiPrep16Plus

Specifications

Dimensions (cm) 32 (W) x 48.7 (D) x 53.5 (H)
Weight (kg) 22
Voltage / Frequency 100 - 240 VAC, 50/60 Hz
Heat block 40 - 90°C
Temperature range 15 - 35°C
Humidity range 20 - 80%, no condensation
Operating system Standalone
Communications TCP/IP
User interface 320 x 240 touch screen graphic LCD
UV sterilization 15 minute cycle

* Specifications of this product are subject to change without notice to improve product performance.

Comparisons

ExiPrep™ 16 Plus ExiProgen
DNA/RNA Extraction O O
Protein Synthesis X O
Protein Purification O
TFT touch screen O O
Sample 16 samples 16 samples X Number of instrument
UV sterilization O O
Heater & magnetic block O O
Contamination shield of catridge O O
TCP/IP network X Numerous instrument can be connected
Cooling system for sample store X O
Automatic aliquoting X O
Power supply 24 VDC, 2.7A(60W) 24 VDC, 7.5A(180W)
weight 22 kg 27 kg

* Unlike ExiProgen™, ExiPrep™ 16 Plus does not offer storing function.
  you can use ExiPrep™ 16 pluss in the cold chamber or cold room.

Experimental Data


1Genomic DNA from cultured mammalian cell (HeLa)

  • Sample volume: 1X106 cells
  • Protocol number: 109
  • Total prep time: about 1 hr 30 min.
  • Average yield: 4-8 μg
ExiPrep16Plus

Agarose gel analysis

ExiPrep16Plus

Figure 1. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X106 cells of cultured HeLa cell and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.8 and 2.0 (not shown).



2Genomic DNA from bacteria (E.coli)


  • Sample volume: 1X109 cells
  • Protocol number: 109
  • Total prep time: about 1 hr 40 min.
  • Average yield: 8-12 µg
ExiPrep16Plus

Agarose gel analysis

ExiPrep16Plus

Figure 2. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X109 cells of E.coli cells and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).



3Genomic DNA from tissue (bovine skeletal muscle)


  • Sample volume: ~ 25 mg
  • Protocol number: 102
  • Total prep time: about 1 hr 30 min.
  • Average yield: Up to 20 µg
ExiPrep16Plus

Agarose gel analysis

ExiPrep16Plus

Figure 3. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 25 mg of bovine tissues and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).



4Genomic DNA from plant tissue (spinach leaf)


  • Sample volume: ~ 100 mg
  • Protocol number: 104
  • Total prep time: about 1 hr
  • Average yield: Up to 5 µg
ExiPrep16Plus

Agarose gel analysis

ExiPrep16Plus

Figure 4. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 100 mg of spinach leaves and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).



5RNA extraction from cultured mammalian cells (HeLa).


  • Sample volume: 1.0 X 106 cells
  • Protocol number: 202
  • Average yield: Up to 10 µg
  • Average RQS: 9.5 or more
ExiPrep16Plus

Electrophoresis result with LabChip GX

ExiPrep16Plus

Figure 5. Results of capillary electrophoresis of total RNA extracted from HeLa cell (1X106 cells) using LabChip GX (Caliper life science, USA). Average RQS (RNA Quality Score) were at least 9.5 shown.



Real-time RT-PCR results of total RNA extracted from cultured cell

ExiPrep16Plus

Figure 6. Real-time RT-PCR results using an AccuPower® RocketScrpt ™ RT PreMix (K-2101, Bioneer)와 AccuPower® Dualstar ™ qPCR PreMix (K-6110, Bioneer) and run on Exicycler ™ 96 Real-Time PCR System (A-2060, Bioneer) from total RNA extracted from HeLa cell (1X106 cells)




Cat. No. Product Description Price
A-5030 ExiPrep ™ 16 Plus Inquire

Related Products

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K-4217 ExiPrep™ Plus Rice Genomic DNA Kit, 96 reactions
K-4244 ExiPrep™ Plus Plant total RNA Kit, 96 reactions
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Cat. No. Accessories
A-5030-A Accessories Set for ExiPrep™ 16 Plus (A2,3,4,6,7,10)
A-5030-A1 Multi Puncher (option)
A-5030-A2 Setup Tray
A-5030-A3 Disposable Tip Rack
A-5030-A4 Elution Tube Rack
A-5030-A6 Waste Tray
A-5030-A7 Hole Puncher (6-hole)
A-5030-A9 AC Adapter for ExiPrep16 Plus
A-5030-A10 Contamination Shield
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