AccuPrep® Genomic DNA Extraction Kit (100 reactions)

AccuPrep^® Genomic DNA Extraction Kit allows rapid extraction of genomic DNA from blood, animal tissues, and cultured cells. The isolated genomic DNA can be used for various experiments such as gene cloning, PCR, quantitative real time PCR, southern blotting, and etc.

Spin column type

$124.00

Cat. No.

K-3032

Product Overview

Features and Benefits

Comprehensive sample compatibility
High-quality, high-yield genomic DNA extraction from various samples such as blood, animal tissues, cultured cells, etc.
Optimized for various animal tissue lysis
Lysis Buffer (TL Buffer) included
High binding capacity
Up to 30 µg using silica based DNA binding column
Convenience
Ethanol already added to the Wash Buffer

Application

• Gene cloning

• PCR

• Real-time PCR

• Southern blotting

• SNP genotyping

Technical/Specs

▶ Specifications

Sample	Amount	DNA Yield	^Purity
Whole blood	200 µl	3 - 6 µg	A_260/280 > 1.8
Buffy coat	200 µl	15 - 20 µg
Cultured cells	10⁴ - 10⁸ cells	15 - 20 µg
Mammalian tissue	25 - 50 mg	10 - 15 µg

^* Minor differences in the size may be found in the actual product.

▶ Components

Components	K-3032
TL Buffer (Tissue Lysis)	35 ml X 1 ea
GB Buffer (Binding)	30 ml X 1 ea
WA1 Buffer (1^st Washing)	40 ml X 1 ea
W2 Buffer (2^nd Washing)	80 ml X 1 ea
EA Buffer (Elution)	25 ml X 1 ea
RNase A powder, lyophilized	24 mg X 2 ea
Proteinase K, lyophilized	25 mg X 2 ea
AccuPrep^® Binding Column-I	100 ea
Collection tubes (for filtration)	100 ea
1.5 ml Tubes (for elution)	100 ea
User's Guide	1 ea

▶ Procedure

▶ Experimental data

Figure 1. Gel electrophoresis of whole blood genomic DNA

- Lane M1; Molecular weight marker, λ DNA/EcoR l + Hind lll markers (Cat. No. D-1070, Bioneer)
- M2; Molecular weight marker, 1 kb ladder (Cat. No. D-1040, Bioneer)
- Lane 1; Whole blood genomic DNA of two individual mice
- Lane 2; Whole blood genomic DNA of two individual rats
- Lane 3; Whole blood genomic DNA of two persons.
* 100 ng of intact DNA (left) and fragment DNA digested with Hind III (E-1721, Bioneer) enzyme (right)

Figure 2. PCR with p53 primer set after genomic DNA purification

- Lane M; Molecular marker, 100 bp ladder (D-1030, Bioneer)
- Lane 1~8; PCR product with p53 primer set

Literature/Support

◈ Manual

• Manual_AccuPrep^® Genomic DNA Extraction Kit

◈ Brochure

• AccuPrep^® nucleic acid extraction kit series

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

Cat. No.	Product Description	Option	Price
K-3032	AccuPrep^® Genomic DNA Extraction Kit	100 reactions	$124.00
KB-0111	Proteinase K Powder	25 mg X 2	$70.00