DUALED Blue/White Transilluminator

DUALED Blue / White Transilluminator is designed innovatively with highly sensitive dual light sources to observe nucleic acids stained fluorescently with dyes such as GreenStar™ Nucleic Acid Staining Solution I (Cat. No. C-9036, Bioneer) using 470 nm blue LED light. This product can be also used for visualizing proteins on SDS-PAGE gel stained with Coomassie Brilliant Blue staining solution using white LED light. Furthermore, with the use of LED light harmless to human health, it can provide safe lab environment during agarose gel cutting and data imaging analysis.

$800.00

Cat. No.

A-6020

Product Overview

Features and Benefits

Dual LED light sources
- Blue LED light: Visualizing nucleic acids dyed with fluorescent staining reagents
- White LED light: Visualizing proteins dyed with Coomassie blue staining solution on SDS-PAGE gel
Magnetic filters
Angle adjustments (0 °, 60 °) and detachment features for convenient sample observation and gel cutting
Bottom-Up LED Illumination
Clear observation of results without the background
Three-stage brightness adjustment
Light intensity adjustment into 3 different modes
Automatic shut-down function
Economical power saving with automatic shut-down function if left unused for more than 5 minutes
Convenient design
Lightweight and compact design for easy moving and storing

Application

Identifying nucleic acid
Cloning, PCR, gel cutting, purification, sequencing
Identifying proteins
SDS-PAGE, western blot X-ray film

Technical/Specs

▶ Components

Number	Components
①	DUALED Blue/White Transilluminator base
②	Amber filter cover
③	Blue uniform plate
④	White uniform plate
⑤	Power cord
⑥	Gel-cutting knife and replacement blade
⑦	Mini darkroom

▶ Product Specifications

Specifications
Instrument dimensions, W x L x H (cm)	18.5 x 22 x 3
Viewing surface dimensions, W x L (cm)	12 x 18
LED wavelength (nm)	470 nm
Lid / filter	Amber (580 nm)
LED life (hours)	> 30,000
Certifications	CE/ETL
Input voltage	100-240 Vac
Input current	2.0 A
Automatic power-off	5 min
Unit weight (kg)	2.27 kg
The number of LED lamps	36 blue-light LED lamps/24 white-light LED lamps
Package set	Gel-cutting knife, replacement blade, mini darkroom

▶ Experimental method

• Blue Light Mode

Agarose Gel Cutting	Agarose Gel Imaging

• White Light Mode

SDS-PAGE Gel Imaging

▶ Experimental data

Blue Light Mode (Nucleic Acid Analysis)

Fig. 1. Check the performance of the blue-light LED using the GreenStar™ Natural Acid Staining Solution I (Cat. No. C-9036, Bioneer).

White Light Mode (Protein Analysis)

Fig. 2. Check white-light LED performance using Coomassie Blue staining solution (without destaining step)

Literature/Support

◈ Manual

• Manual_DUALED Blue/White Transilluminator

◈ Brochure

• BIONEER - Instruments & Devices

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 13485 - certificate

Ordering Info

Cat. No.	Product Description
A-6020	DUALED Blue/White Transilluminator

FAQ

Checking nucleic acids

How do I set this equipment for DNA verification?

Set this equipment in this following order. You can film with your smartphone afterwards.
DUALED Blue/White Transilluminator base → Blue uniform plate → Agarose gel → Amber filter cover → Mini darkroom

Are the blue uniform plate and the amber filter cover crucial when checking the DNA bands?

Yes, those must be used. While the blue uniform plate is used for filtering LED light to 470 nm wavelength bands for excitation of fluorescent dye components attached to the DNA samples, the amber filter cover is for blocking the excitation spectrum for clear visualization of fluorescent without any light interference.

Is the mini darkroom necessary during DNA observation?

It’s not essential, but it allows more clear observation through blocking reflected ambient light.

Can I cut the DNA bands directly from an agarose gel on the blue uniform plate?

Yes, its upper part where the blade touches is made with scratch-resistant materials. However, it is advised to handle with care as the blade may crack the glass if pressed too hard.

The intensity of DNA stained with fluorescent dyes seems to be low.

Make sure that the fluorescent dyes have an excitation wavelength of 400 ~ 500 nm
Try increasing the sample concentration as its insufficiency may hinder the DNA visualization
Adjust the sample fluorescent intensity by using the 3 step brightness control button on the main unit of transilluminator base
Change brightness, contrast, and exposure time to adjust the fluorescence intensity when taking images using smartphones.
Use Mini Darkroom which can provide more clear visualization of DNA by blocking the reflected ambient light

Checking proteins

How do I set this equipment for DNA verification?

Set this equipment in this following order. You can film with your smartphone afterwards.
DUALED Blue/White Transilluminator base → White uniform plate → SDS-PAGE gel → Mini darkroom

What is the purpose of the white uniform plate?

It protects the DUALED Blue/White Transilluminator base from staining by dye and seeping of moisture through the base. Furthermore, it enhances the visualization of SDS-PAGE data.

Can the protein bands on the SDS-PAGE gel dyed with Coomassie blue staining be visible without destaining?

Yes, those can be seen easily without the staining process by adjusting the camera brightness, contrast, and exposure time.

Using the mini darkroom allow clear visualization through protection from the surrounding light.

Regarding the customer service / warranty (A/S)

How does the warranty work on the defective machines?

The warranty lasts for one year from the purchase date. During this period, any faulty products or parts will be repaired or replaced for a free of charge. Please contact our customer service center by calling 1588-9788 or sending an email to sales@bioneer.co.kr for details.
However, free warranty will not be supported under those circumstances:
- In the case of disassembling, changing, or breaking the device user’s own will (in such cases, the repair may be charged or denied)
- In the event of natural disaster
- In the case of machine failure caused by user’s carelessness or mishandling
- In the case of repair by a person other than an authorized agent or a BIONEER employee
- In case of using accessories supplied by people from other than BIONEER
- In case of being damaged by replacement of spare parts
If the warranty period has expired, there will be charges for repairs or replacement of defective products. Please call customer service at Tel. 1588-9788 or email at sales@bioneer.co.kr and specify the details. We will contact you back after our inspection.

If you have any additional questions, please contact us at sales@bioneer.co.kr