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AccuPrep® Genomic DNA Extraction Kit (100 reactions)
AccuPrep® Genomic DNA Extraction Kit allows rapid extraction of genomic DNA from blood, animal tissues, and cultured cells. The isolated genomic DNA can be used for various experiments such as gene cloning, PCR, quantitative real time PCR, southern blotting, and etc.
Spin column type
Features and Benefits
-
Comprehensive sample compatibility
High-quality, high-yield genomic DNA extraction from various samples such as blood, animal tissues, cultured cells, etc. -
Optimized for various animal tissue lysis
Lysis Buffer (TL Buffer) included -
High binding capacity
Up to 30 µg using silica based DNA binding column
Application
• Gene cloning
• PCR
• Real-time PCR
• Southern blotting
• SNP genotyping
▶ Specifications
Sample | Amount | DNA Yield | Purity |
Whole blood | 200 µl | 3 - 6 µg | A260/280 > 1.8 |
Buffy coat | 200 µl | 15 - 20 µg | |
Cultured cells | 104 - 108 cells | 15 - 20 µg | |
Mammalian tissue | 25 - 50 mg | 10 - 15 µg |
* Minor differences in the size may be found in the actual product.
▶ Components
Components | K-3032G |
TL Buffer (Tissue Lysis) | 35 ml X 1 ea |
GB Buffer (Binding) | 30 ml X 1 ea |
WA1 Buffer (1st Washing) | 40 ml X 1 ea |
WB2 Buffer (2nd Washing) | 16 ml X 1 ea |
EA Buffer (Elution) | 25 ml X 1 ea |
RNase A powder, lyophilized | 24 mg X 2 ea |
Proteinase K, lyophilized | 25 mg X 2 ea |
AccuPrep® Binding Column-I | 100 ea |
Collection tubes (for filtration) | 100 ea |
1.5 ml Tubes (for elution) | 100 ea |
User's Guide | 1 ea |
▶ Procedure
▶ Experimental data
Figure 1. Gel electrophoresis of whole blood genomic DNA
- Lane M1; Molecular weight marker, λ DNA/EcoR l + Hind lll markers (Cat. No. D-1070, Bioneer)
- M2; Molecular weight marker, 1 kb ladder (Cat. No. D-1040, Bioneer)
- Lane 1; Whole blood genomic DNA of two individual mice
- Lane 2; Whole blood genomic DNA of two individual rats
- Lane 3; Whole blood genomic DNA of two persons.
* 100 ng of intact DNA (left) and fragment DNA digested with Hind III (E-1721, Bioneer) enzyme (right)
Figure 2. PCR with p53 primer set after genomic DNA purification
- Lane M; Molecular marker, 100 bp ladder (D-1030, Bioneer)
- Lane 1~8; PCR product with p53 primer set
◈ Manual
• Manual_AccuPrep® Genomic DNA Extraction Kit
• One Page Protocol_AccuPrep® Genomic DNA Extraction Kit
◈ MSDS
• MSDS_AccuPrep® Genomic DNA Extraction Kit TL Buffer EN ver.3
• MSDS_AccuPrep® Genomic DNA Extraction Kit GB Buffer EN ver.2
• MSDS_AccuPrep® Genomic DNA Extraction Kit WA1 Buffer EN ver.2
• MSDS_AccuPrep® Genomic DNA Extraction Kit W2 Buffer EN ver.3
• MSDS_AccuPrep® Genomic DNA Extraction Kit EA Buffer EN ver.2
• MSDS_AccuPrep® Genomic DNA Extraction Kit RNase A EN ver.6
• MSDS_AccuPrep® Genomic DNA Extraction Kit Proteinase K EN ver.7
◈ Brochure
• AccuPrep® nucleic acid extraction kit series
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
Cat. No. | Product Description | Option | Price |
K-3032G | AccuPrep® Genomic DNA Extraction Kit | 100 reactions | $K-3032G |
KB-0111 | Proteinase K Powder | 25 mg X 2 | $KB-0111 |