We use cookies to give you the best online experience. By using our website you agree to our use of cookies in accordance with our Cookie Policy
Proteinase K Powder for K-3031/3032/3036 (25 mg x 2)
This product is delivered in lyophilized form which is added in a solution for protein decomposition during DNA and/or RNA extraction process. It is highly recommended to store at 4℃ or -20℃ to mantain its enzymatic activities.
Features and Benefits
-
Comprehensive sample compatibility
High-quality, high-yield genomic DNA extraction from various samples such as blood, animal tissues, cultured cells, etc. -
Optimized for various animal tissue lysis
Lysis Buffer (TL Buffer) included -
High binding capacity
Up to 30 µg using silica based DNA binding column
Application
• Gene cloning
• PCR
• Real-time PCR
• Southern blotting
• SNP genotyping
▶ Specifications
Sample | Amount | DNA Yield | Purity |
Whole blood | 200 µl | 3 - 6 µg | A260/280 > 1.8 |
Buffy coat | 200 µl | 15 - 20 µg | |
Cultured cells | 104 - 108 cells | 15 - 20 µg | |
Mammalian tissue | 25 - 50 mg | 10 - 15 µg |
* Minor differences in the size may be found in the actual product.
▶ Components
Components | K-3032G |
TL Buffer (Tissue Lysis) | 35 ml X 1 ea |
GB Buffer (Binding) | 30 ml X 1 ea |
WA1 Buffer (1st Washing) | 40 ml X 1 ea |
WB2 Buffer (2nd Washing) | 16 ml X 1 ea |
EA Buffer (Elution) | 25 ml X 1 ea |
RNase A powder, lyophilized | 24 mg X 2 ea |
Proteinase K, lyophilized | 25 mg X 2 ea |
AccuPrep® Binding Column-I | 100 ea |
Collection tubes (for filtration) | 100 ea |
1.5 ml Tubes (for elution) | 100 ea |
User's Guide | 1 ea |
▶ Procedure
▶ Experimental data
Figure 1. Gel electrophoresis of whole blood genomic DNA
- Lane M1; Molecular weight marker, λ DNA/EcoR l + Hind lll markers (Cat. No. D-1070, Bioneer)
- M2; Molecular weight marker, 1 kb ladder (Cat. No. D-1040, Bioneer)
- Lane 1; Whole blood genomic DNA of two individual mice
- Lane 2; Whole blood genomic DNA of two individual rats
- Lane 3; Whole blood genomic DNA of two persons.
* 100 ng of intact DNA (left) and fragment DNA digested with Hind III (E-1721, Bioneer) enzyme (right)
Figure 2. PCR with p53 primer set after genomic DNA purification
- Lane M; Molecular marker, 100 bp ladder (D-1030, Bioneer)
- Lane 1~8; PCR product with p53 primer set
◈ Manual
• Manual_AccuPrep® Genomic DNA Extraction Kit
• One Page Protocol_AccuPrep® Genomic DNA Extraction Kit
◈ MSDS
• MSDS_AccuPrep® Genomic DNA Extraction Kit TL Buffer EN ver.3
• MSDS_AccuPrep® Genomic DNA Extraction Kit GB Buffer EN ver.2
• MSDS_AccuPrep® Genomic DNA Extraction Kit WA1 Buffer EN ver.2
• MSDS_AccuPrep® Genomic DNA Extraction Kit W2 Buffer EN ver.3
• MSDS_AccuPrep® Genomic DNA Extraction Kit EA Buffer EN ver.2
• MSDS_AccuPrep® Genomic DNA Extraction Kit RNase A EN ver.6
• MSDS_AccuPrep® Genomic DNA Extraction Kit Proteinase K EN ver.7
◈ Brochure
• AccuPrep® nucleic acid extraction kit series
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
Cat. No. | Product Description | Option | Price |
K-3032G | AccuPrep® Genomic DNA Extraction Kit | 100 reactions | $K-3032G |
KB-0111 | Proteinase K Powder | 25 mg X 2 | $KB-0111 |