AccuPower® HotStart Pfu PCR PreMix
AccuPower® HotStart Pfu PCR PreMix is applied with Bioneer's enzyme-mediated HotStart patent technology to enhance response specificity and PCR amplification, along with reduction of non-specific reactions such as mis-priming and primer-dimer that may occure during Pfu DNA polymerase reaction at low temperatures. In addition, the proof-reading (3 '→ 5' exonuclease) function of Pfu DNA polymerase reduces the error in DNA amplification reaction, making it suitable for cloning experiments for protein production.
Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer.
※ This product is shipped at room temperature.
※ The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
Features and Benefits
High fidelity (error rate= 1.9X10-6) with minimized mutation during DNA amplification
Selection of specific sequences by applying PyroHotStart technology to the proof-reading function of Pfu DNA polymerase, allowing the amplified products to be used in experiments that require accurate sequences such as cloning and mutagenesis after simple PCR purification processes
Simplified procedure by requiring only the addition of template DNA, primer sets, and D.W., with all necessary components and DNA polymerases included and mixed in each of the tubes packaged with sufficient amount for 1 PCR run, along with application of Hotstart technology for amplifying specific targets accurately to minimize tedious steps such as Gel extraction for cloning
Stable enzyme activities allowing long-term storage by including stabilizer and dried in the PCR reaction mixture
Minimized deviation between lots through a mass production under ISO 9001 quality system, allowing reproducible results even for replicates tested repeatedly under same conditions and variables
• Gene cloning with blunt ends
• Site-directed mutagenesis
• High fidelity amplification
• High specificity PCR
• cDNA amplification
Figure. Comparison of PCR amplification efficiency between AccuPower® Hotstart Pfu PCR PreMix from Bioneer and other suppliers' PCR master mix
Target : human insulin receptor gene. The cycling conditions for AccuPower® Hotstart Pfu PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 30 sec, 55°C for 30 sec and 72°C for 2min. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
Lane M ; 100 bp DNA Ladder(Cat. No. D-1030,Bioneer)
Lane 1 ; 10 ng of human genomic DNA
Lane 2 ; 1 ng of human genomic DNA
Lane 3 ; 100 pg of human genomic DNA
Lane 4 ; 10 pg of human genomic DNA