GreenStar™ Nucleic Acid Staining Solution I

GreenStar™ Nucleic Acid Staining Solution I serves as an alternative to Ethidium Bromide (EtBr) for a safe nucleic acid staining reagent harmless to humans. It is provided with 200X concentration with 50 mL volume and can be used about 100 times. This product is used for pre-casting gel and post staining. With its high sensitivity, nucleic acids can be clearly seen even in concentration below 5 ng/band. The wavelengths for excitation and emission are 496 nm and 522 nm. Either UV-transilluminator or DUALED Blue / White Transilluminator is used for visualization of stained nucleic acids, but the latter is recommended for higher sensitivity.

※This product is shipped in dry ice.

$59.00
In stock
SKU
C-9036

Features and Benefits

  • Safety

    Harmless fluorescent dyes capable of serving as a safe replacement for EtBr solutions which, on the other hand, have a risk of DNA mutation

  • High sensitivity

    Highly sensitive fluorescent dyes allowing observation of nucleic acids even in miniscule quantities less than 5 ng / band (DUALED Blue / White Transilluminator recommended)

  • Low DNA damage

    Minimized DNA damage during the staining process through the use of DUALED Blue / White Transilluminator during the observation of nucleic acid 

  • Cost-efficiency 

    Low price compared to other products while using traditional UV-transilluminator or DUALED Blue / White Transilluminator without the need of additional equipments. 

  • Convenience

    Same procedure of gel pre-casting and nucleic acid post-staining as using EtBr solution; de-staining stage during the post-staining step is not required

  • Reproducibility

    Meticulous production after stringent QC processes under ISO 9001 quality systems providing reproducible test results.

Applications

  • Cloning

  • PCR

  • Sequencing

  • Electrophoresis

Components

Component Specifications Storage temperature
Main product 50 ml x 1 ea (200X) -20℃ (Daylighting required)
Manual 1 ea -

 




 Experimental data

- Pre-casting gel

Figure 1. Comparison of fluorescence sensitivities of 1X GreenStar™ Nucleic Acid Staining Solution I and 0.5 μg/ml EtBr using a 100 bp Plus DNA Ladder (Cat. No. D-1035, Bioneer) (pre-casting gel).

 - Post-staining gel

Figure 2. Comparison of fluorescence sensitivities of  5X GreenStar™Nucleic Acid Staining Solution I with 100 bp Plus DNA Ladder (Cat. No. D-1035, Bioneer) and a competitor's product (T company, post-staining gel). 

Manual

Manual_GreenStar™ Nucleic Acid Staining Solution I

MSDS

MSDS_GreenStar™ Nucleic Acid Staining Solution I

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate

Cat. No. Product Description Price
C-9036 GreenStar™ Nucleic Acid Staining Solution I, 50 ml $59.00

▶ During pre-casting of gels

  • Completely dissolve 1 g of agarose in 100 mL 0.5X TBE or 1X TAE Buffer to make a 1% agarose, 1X pre-casting gel. Then, add 500 μl of GreenStar ™ Nucleic Acid Staining Solution I and mix well. Pour into a gel mold and wait until it hardens. After using the gel for electrophoresis, clear bands of nucleic acids can be observed through DUALED Blue/White Transilluminator.
  • This is because the optimal wavelength of EtBr for excitation is near the UV range while GreenStar™ Nucleic Acid Staining Solution I is near the blue spectrum band. Therefore, stained DNA produces stronger fluorescence under UV transilluminator for the former stain while the latter is more visible under DUALED Blue/White Transilluminator. Nevertheless, if the stained DNA is viewed under the equipments capable of producing light under the solution’s optimal spectrum band, it can be seen that the GreenStar™ Nucleic Acid Staining Solution I can visualize the bands more clearly than EtBr.
  • Cause: This may be due to the amphoteric ions included in the GreenStar ™ Nucleic Acid Staining Solution I staining solution. The binding of fluorescence dye with the DNA reduces mobility during the electrophoresis process. This is exceptionally the case when a large amount of nucleic acids are loaded into the gel, as the dye is not completely bound to the samples. With the mixture of bound and unbound samples, the DNA band separation may not be performed well.
  • Suggestion 1: As stated in the manual, it is recommended that when being loaded on a pre-cast gel, the DNA samples do not to go over the concentration of 20 ng/band.
  • Suggestion 2: If DNA samples must go over concentration of 20 ng/band for the electrophoresis, it is highly recommended to use an agarose gel without fluorescent dyes, and then undergo the staining process afterwards. 
  • The amphoteric ions included in the fluorescent dye of GreenStar™ Nucleic Acid Staining Solution I lower the speed of DNA fragments. The additional time required depends on the size of the agarose gel, but using the solution typically takes about extra 10 minutes than applying EtBr.
  • Due to the fluorescent dye included in the solution, GreenStar™ Nucleic Acid Staining Solution I may be broken down in proportion to the amount of light irradiation. If the gel will not be used immediately, store at -20℃ to 4℃ in a dark condition to reduce its photodegradation. same type of buffer and electrophoresis buffer used to make the agarose gel. When using buffers of different composition, the concentration of DNA loaded on the gel may decrease as ions of the gel and buffer solution diffuse.
  • During the agarose gel preparation process, buffers for its manufacture and the electrophoresis must be the same. If the buffer compositions for the two are different, the concentration of loaded DNA may decrease due to the diffusion process of the ions of the gel and the buffer solution.

 

▶ During post-casting of gels

  • Completely dissolve 1 g of agarose in 100 mL 0.5X TBE or 1X TAE Buffer to make a 1% agarose, 1X pre-casting gel. Then, add 500 μl of GreenStar ™ Nucleic Acid Staining Solution I and mix well. Pour into a gel mold and wait until it hardens. After using the gel for electrophoresis, clear bands of nucleic acids can be observed through DUALED Blue/White Transilluminator.
  • This is because the optimal wavelength of EtBr for excitation is near the UV range while GreenStar™ Nucleic Acid Staining Solution I is near the blue spectrum band. Therefore, stained DNA produces stronger fluorescence under UV transilluminator for the former stain while the latter is more visible under DUALED Blue/White Transilluminator. Nevertheless, if the stained DNA is viewed under the equipments capable of producing light under the solution’s optimal spectrum band, it can be seen that the GreenStar™ Nucleic Acid Staining Solution I can visualize the bands more clearly than EtBr.
  • Cause: The fluorescence intensity is inversely proportional to the thickness of the manufactured agarose gel: thicker the gel, lower the fluorescence intensity.
  • Suggestion 1:: Extend the dyeing time of the gel (10 ~ 40 minutes) or increase the concentration of the staining solution (1X - 10X Recommended, 1/20 - 1/200 Diluted)
  • Suggestion 2: Use gels with thickness less than 0.5 cm
  • Suggestion 3: Adjust the exposure time to get the desired image when using Transilluminator for gel photography
  • Due to the fluorescent dye included in the solution, GreenStar™ Nucleic Acid Staining Solution I may be broken down in proportion to the amount of light irradiation. If the gel will not be used immediately, store at -20℃ to 4℃ in a dark condition to reduce its photodegradation.
  • If dyeing performance deteriorates, it is recommended that you use the new staining solution.
  • During the agarose gel preparation process, buffers for its manufacture and the electrophoresis must be the same. If the buffer compositions for the two are different, the concentration of loaded DNA may decrease due to the diffusion process of the ions of the gel and the buffer solution.
  • Excessive staining may cause the bands to smear. This is due to the high sensitivity of GreenStar™ Nucleic Acid Staining Solution I. Reduce the dyeing time or the concentration of the staining solution.
  • Smearing may also occur if the DNA is stained in the degraded state

For more information, please contact sales@bioneer.co.kr .

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