- Completely dissolve 1 g of agarose in 100 mL 0.5X TBE or 1X TAE Buffer to make a 1% agarose, 1X pre-casting gel. Then, add 500 μl of GreenStar ™ Nucleic Acid Staining Solution I and mix well. Pour into a gel mold and wait until it hardens. After using the gel for electrophoresis, clear bands of nucleic acids can be observed through DUALED Blue/White Transilluminator.
GreenStar™ Nucleic Acid Staining Solution I
GreenStar™ Nucleic Acid Staining Solution I serves as an alternative to Ethidium Bromide (EtBr) for a safe nucleic acid staining reagent harmless to humans. It is provided with 200X concentration with 50 mL volume and can be used about 100 times. This product is used for pre-casting gel and post staining. With its high sensitivity, nucleic acids can be clearly seen even in concentration below 5 ng/band. The wavelengths for excitation and emission are 496 nm and 522 nm. Either UV-transilluminator or DUALED Blue / White Transilluminator is used for visualization of stained nucleic acids, but the latter is recommended for higher sensitivity.
※This product is shipped in dry ice.
Features and Benefits
Harmless fluorescent dyes capable of serving as a safe replacement for EtBr solutions which, on the other hand, have a risk of DNA mutation
- High sensitivity
Highly sensitive fluorescent dyes allowing observation of nucleic acids even in miniscule quantities less than 5 ng / band (DUALED Blue / White Transilluminator recommended)
- Low DNA damage
Minimized DNA damage during the staining process through the use of DUALED Blue / White Transilluminator during the observation of nucleic acid
Low price compared to other products while using traditional UV-transilluminator or DUALED Blue / White Transilluminator without the need of additional equipments.
Same procedure of gel pre-casting and nucleic acid post-staining as using EtBr solution; de-staining stage during the post-staining step is not required
Meticulous production after stringent QC processes under ISO 9001 quality systems providing reproducible test results.