AccuPower® RocketScript™ RT PreMix, RNase H Minus uses RocketScript™ RTase and RNase H Minus developed by Bioneer independently. By using unique genetic engineering technology, it is useful for cDNA synthesis of long target RNA by removing RNase H activity. It is excellent in extensibility and sensitivity, and can successfully synthesize cDNA even in 1 pg human total RNA. It also contains all the components necessary for cDNA synthesis, so RT-reactions can be performed directly by adding RNA to be amplified and D.W.
※This product is shipped in dry ice.
Time-efficient and economical synthesis of cDNA from secondary structure RNA with RocketScript™ RNase having excellent thermal stability capable of being active at even 70 ℃
Useful for cDNA synthesis up to 12.5 kb from long target RNA using enzymes developed by proprietary genetic engineering technology
High sensitivity and effective synthesis of cDNA even at scarce amount of 1 pg of human total RNA.
Synthesis of cDNA samples difficult to be done using traditional methods, such as those made with long size RNA (9 kb), possible only within 10 minutes
Minimized deviation between lots, then supplied as uniform quality product to provide reproducible experimental results by mass production under ISO 9001 quality system
Application
• First-strand synthesis of cDNA from RNA molecules (RT)
• Random priming reaction
• cDNA library construction
• Probe labeling
• mRNA 5‘-end mapping by primer extension analysis
• PCR
• Real-time qPCR
Figure. Performance of the AccuPower® RocketScript ™ RT PreMix, RNase H Minus in two-step RT-PCR.
Target mRNAs ranged from 500 bp to 11 kb were reverse transcribed by using AccuPower® RocketScript ™ RT PreMix, RNase H Minus. With each cDNA, PCR was performed by using AccuPower® ProFi Taq PCR PreMix (Cat. No. K-2631, Bioneer). Template sizes are indicated above the gel image.
M1; 1 kb DNA Ladder (Cat. No. D-1040, Bioneer)
M2; Lambda DNA/Hind III Markers (Cat. No. D-1050, Bioneer)
