Our use of cookies We use cookies to give you the best online experience. By using our website you agree to our use of cookies in accordance with our Cookie Policy
BOINEER’s Ni-NTAMagnetic Beads are coated with high-density of Nickel-nitrilotriacetic acid (Ni-NTA) on the surface of Silica Magnetic Beads. Histidine residues in the His tag have a strong affinity for nickel ions, and Nitrilotriacetic acid (amino-polycarboxylic acid, N(CH2CO2H)3) works as a chelating agent. Ni-NTA Magnetic beads can purify 6x His-tagged proteins. Using the MagListo™ Magnetic Separation Rack (TM-1000, TM-1010, TM-1011, TM-1020, TM-1030) or other magnetic separation racks, biomolecules attached to the beads can be easily purified by magnetic force.
BOINEER’s Ni-NTAMagnetic Beads are coated with high-density of Nickel-nitrilotriacetic acid (Ni-NTA) on the surface of Silica Magnetic Beads. Depending on the size, Biotin Magnetic Beads are available as micrometer-sized AccuBead™ (1 - 5 μm) and nanometer-sized AccuNanoBead™ (400 nm). All magnetic beads developed by BIONEER have a pure magnetite (iron(Ⅱ, Ⅱ) oxide, Fe3O4) core. Compared to other beads that use polymer cores, BIONEER’s magnetic beads have higher magnetic saturation, allowing them to be collected more quickly by a magnet. This enables faster separation and purification of target biomolecules. Additionally, BIONEER’s technology allows for more functional groups to be attached to the surface, increasing the efficiency of experiments by capturing more target biomolecules with the same volume as magnetic beads. Histidine residues in the His tag have a strong affinity for nickel ions, and Nitrilotriacetic acid (amino-polycarboxylic acid, N(CH2CO2H)3) works as a chelating agent. Ni-NTA Magnetic beads can purify 6x His-tagged proteins. Using the MagListo™ Magnetic Separation Rack (TM-1000, TM-1010, TM-1011, TM-1020, TM-1030) or other magnetic separation racks, biomolecules attached to the beads can be easily purified by magnetic force.
Features & Benefits
A high magnetic density of 80.8 emu/g derived from a pure magnetite(Fe3O4) core
Rapid magnetic capture within 5 seconds with a high magnetic density
(Black - BIONEER AccuNanoBead™, Brown - Competitor's polymer core product)
A high binding capacity of target molecules because of highly dense functional groups and smaller size
Microplastics-free as no plastic cores like polystyrene
Applications
Isolating and purifying Histidine-tagged peptides and proteins from samples
Add Magnetic beads after loading protein samples in the tube. Then, gather magnetic beads by external magnetic field through magnetic separation rack, and wash the proteins by washing solutions. After several washing steps, elute the proteins from the magnetic beads. Protein purification using magnetic beads allows for faster protein separation compared to traditional methods.
Figure 1. The process of protein purification using magnetic beads
▶ Electron microscopy image (FE-SEM and TEM)
Figure 2. FE-SEM images of AccuNanobead™. Through them, it is possible to confirm that AccuNanobead™ is close to a spherical shape.
▶ Size distribution
Figure 3. Size distribution of AccuNanoBead™. It can be seen that the average size of AccuNanoBead™ is about 400 nm.
▶ Comparison of magnetic beads shapes
Figure 4. Comparison of BIONEER's nano-sized magnetic beads(a) and competitors' magnetic beads(b~e) through electon microscopy images. It shows BIONEER's magnetic beads are more round shape than competitors' magnetic beads. (a) BIONEER's magnetic beads (b) Company A's magnetic beads (c) Company B's magnetic beads (d) Company C's magnetic beads (e) Company D's magnetic beads
▶ Protein Purification Efficiency
Protein yields after purification by BIONEER's Ni-NTA magnetic beads are summarized in Table 1. It shows BIONEER's Ni-NTA beads yield 77% more proteins. The proteins used in the experiements was 17kDa of His-Tagged protein, and yields of target proteins are the same as the amount of loading protein.
Loading Protein (mg)
Elution Protein (mg)
Purification Yield (5)
0.4
0.31
77.1
Table 1. Protein Purification Efficiency of Ni-NTA Magnetic Beads(30 mg)
Figure 5. SDS-PAGE results ofpurified 17 kDa of His-Tagged Protein by BIONEER's Ni-NTA magnetic beads. (Gel - 15% Polyacrylamide Gel, Loading volume - 10 μl).
