AccuPower® PyroHotStart Taq PCR PreMix is applied with Bioneer's enzyme-mediated HotStart patent technology to enhance response specificity and PCR amplification. Taq DNA polymerase reduces non-specific reactions like the mis-priming and primer dimer formation that may occur at a low temperature. In addition, the components necessary for PCR such as DNA polymerases, dNTPs, reaction buffers, etc. are thoroughly mixed and vacuum-dried, each packaged with amounts sufficient for a single PCR run.
Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer.
※PreMix product is shipped at room temperature.
※The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
Minimized generation of non-specific amplification products, maximized PCR reaction efficiency, and effective amplification of template DNA present in a small amount through the applicaiton of PyroHotStart technology
Enhanced stability for maintaining stable enzymatic activities for a long-term storage by including stabilizer and dried in the PCR reaction mixture
Simplified procedure with all necessary components and DNA polymerases added in the PCR tubes to perform the PCR reaction immediately just by adding the template DNA, primer sets, and D.W., along with tracking dye and sedimentation agents for electrophoresis without the need of sample loading buffer
Reproducible experimental results provided through mass production of one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product
(a) Inhibition of PCR reaction in the zero cycle
After preparing the PCR mixture, PPi is used to prevent the formation of non-specific reactants before reaching the pre-denaturation temperature.
Also, since DNA polymerases requires Mg ions to activate, PPi, having high affinities with Mg2+ ions, traps them to inhibit the PCR.
PPase included in the mixture is activated when the temperature rises during the denaturation step to decompose PPi into Pi for dissociating the Mg ions.
The isolated ions bind with DNA polymerases to activate, allowing the PCR reaction to proceed.
(c) Annealing & Extension
The activated DNA polymerases amplifies the target DNA as the PCR cycle progresses.
• High specificity PCR
• High sensitivity PCR
• gDNA template PCR
• Low-copy target PCR
• Multiple primer pairs PCR
• cDNA template PCR
• TA cloning
Figure. Comparison of PCR amplification specificity between AccuPower® PyroHotStart Taq PCR PreMix from Bioneer and other supplier's HotStart PCR master mix.
PCR reactions were performed according to each supplier's protocol. The PrP gene was amplified from human genomic DNA with two different primer sets, separately.
This data shows that AccuPower® PyroHotStart Taq PCR PreMix has higher amplificition efficency and specificity than other supplier's HotStart PCR master mix.
Lane M; 100 bp DNA Ladder (Cat. No.D-1030, Bioneer)
Lane 1; 100 ng DNA, PrP primer set (500 bp)
Lane 2; 10 ng DNA, PrP primer set (500 bp)
Lane 3; 100 ng DNA, PrP primer set (705 bp)
Lane 4; 10 ng DNA, PrP primer set (705 bp)