AccuPower® Taq PCR PreMix uses Bioneer's patented technology that combines vacuum-dried components essential for PCR such as Taq DNA polymerases, dNTPs, and reaction buffers. Each tubes contain the mixture with sufficient amounts for one PCR run. Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer.
※Master Mix product is shipped in dry ice.
※PreMix product is shipped at room temperature.
※The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
Addition of stabilizing agents allows delivery at room temperature and enhanced stability compared to solution-type products.
Excellent sensitivity and amplification efficiency even with small amounts of DNA
Simplified procedure having all necessary components for one run vacuum-dried and packaged with sufficient amounts in each of the tubes to perform the PCR reaction immediately just by adding a template DNA and a primer
Minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variables by a mass production under ISO 9001 quality system
• Conventional PCR
• Primer extension
• TA cloning
• Gene sequencing
Figure. Comparison of PCR amplification efficiency between AccuPower® Taq PCR PreMix from Bioneer and other suppliers' PCR master mix.
Target Gene: Human insulin receptor gene; the cycling conditions for AccuPower® Taq PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
Lane M: 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1: 10 ng human genomic DNA
Lane 2: 1 ng human genomic DNA
Lane 3: 100 pg human genomic DNA
Lane 4: 10 pg human genomic DNA
