AccuPower® RT-PCR PreMix & Master Mix

AccuPower^® RT-PCR PreMix utilizes one-step RT-PCR capable of undergoing economical sequential reactions of cDNA synthesis and two-step PCR protocols in a single tube using low-copy RNA (viral RNA) or mRNA. Furthermore, as the each tube contains all the necessary components sufficient for a single run, cross contaminations by the repetitive use of master mix can be avoided.

Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer.

※PreMix product is shipped at room temperature. The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.

※MasterMix product is shipped in dry ice.

As low as $157.00

Cat. No.

Product Overview

Features and Benefits

Easy-of-use
Simplified protocols that allows continous performances of cDNA synthesis and PCR reaction in one tube, only requiring addition of the template RNA and its specific primer
Stability
Addition of stabilizer and RNase inhibitor for high resistance to degradation
Reproducibility
Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables

Application

• First-strand synthesis of cDNA from RNA molecules (RT)

• RT-PCR

• cDNA library construction

• Gene-expression analysis

Experimental data

Figure. Specific amplification of 5'- UTR region of HCV with AccuPower^® RT/PCR PreMix.
Specific amplification of 5' - UTR region of HCV with AccuPower^® RT-PCR PreMix. Human DNA was amplified to generate 0.1-1.5 kb PCR fragments using 1 U of polymerase. The comparison was performed under the same experimental conditions.

Lane 1; DNA Ladder (BM corp.)
Lane 2; negative control
Lane 3, 4; HCV positive serum
Lane 5; HCV negative serum

Technical/Specs

▶ Specifications

5' to 3' exonuclease	No
3' to 5' exonuclease	No
3' – A overhang	Yes
Fragment size	Up to 5 kb

▶ Experimental data

Figure 1. Sensitivity comparison between AccuPower^® RT/PCR PreMix and Supplier I RT-PCR Kit.
Each 10⁹ copy – 10³ copy of PSTVd used for RT/PCR and the same amount of RT/PCR products used for electrophoresis

Lane1; 10⁹ copy Lane2; 10⁸ copy Lane3; 10⁷ copy Lane4; 10⁶ copy
Lane5; 10⁵ copy Lane6; 10⁴ copy Lane7; 10³ copy Lane8; NTC

Figure 2. Comparison of reproducibility test for AccuPower^® RT/PCR PreMix batch 1,2,3 and batch 4 products using serial diluted human total RNA.

Lane 1; 10 ng human total RNA from HeLa cell
Lane 2; 1 ng human total RNA from HeLa cell
Lane 3; 100 pg human total RNA from HeLa cell
Lane 4; 10 pg human total RNA from HeLa cell

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

• ISO 13485 - certificate

Ordering Info

Cat. No.	Product Description	Option			Price
K-2055	AccuPower^® RT-PCR PreMix & Master Mix	PreMix type	0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes	20 µl reaction	$K-2055
K-2057			0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes	50 µl reaction	$K-2057
K-2055-B			0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes	20 µl reaction	$K-2055-B
K-2057-B				50 µl reaction	$K-2057-B
K-2056			0.5 ml thin-wall tubes with attached cap / 100 tubes	50 µl reaction	$K-2056
K-2262-1			thin-wall 96-well flat plate	10 µl reaction	$K-2262-1
K-2262-4			thin-wall 96-well flat plate	20 µl reaction	$K-2262-4
K-2262-2			thin-wall 96-well full-skirted plate	10 µl reaction	$K-2262-2
K-2262-5			thin-wall 96-well full-skirted plate	20 µl reaction	$K-2262-5
K-2262-3			thin-wall 96-well semi-skirted plate	10 µl reaction	$K-2262-3
K-2262-6			thin-wall 96-well semi-skirted plate	20 µl reaction	$K-2262-6
K-2084-1			thin-wall 384-well full-skirted plate	5 µl reaction	$K-2084-1
K-2084-2				10 µl reaction	$K-2084-2
K-2084-3				20 µl reaction	$K-2084-3
K-2264		MasterMix type	1 ml of 2X Master mix solution		$K-2264