HotStart DNA polymerase inhibits PCR by using pyrophosphate (PPi) to trap Mg2+ ions essential for PCR, then breaking the PPi down by using heat resistant enzyme pyrophosphatase (PPase) as the temperature increases while processing the PCR reaction
※This product is shipped in dry ice.
Reduced generation of non-specific responses and primer-dimer formation during the mixing of PCR components by inhibiting DNA polymerase activity at the room temperature
Improved yield of target DNA through its high sensitivity and specificity
Reproducible results with uniform quality products for each batch under the ISO 9001 quality system
Figure. Multiplex PCR comparison of genomic DNA using 6 sets of primers.
Lane M : 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1 : 750 bp fragment
Lane 2 : 590 bp fragment
Lane 3 : 450 bp fragment
Lane 4 : 360 bp fragment
Lane 5 : 260 bp fragment
Lane 6 : 150 bp fragment
Lane 7 : Multiplex PCR with primers used for Lane 1 – 6