AccuPower® GreenStar™ qPCR PreMix
AccuPower® GreenStar™ qPCR PreMix uses dsDNA binding dye in real-time PCR to accurately quantify the target gene samples from various kinds of samples quickly and accurately. It is applied with Bioneer’s patent enzyme-mediated HotStart technology to enhance reaction specificity and PCR amplification efficiency. Furthermore, all the essential components are vacuum dried in each tube to prevent cross-contamination.
This product is applied with PyroHotStart technology, Bioneer’s patent technology, to minimize non-specific reactions by utilizing pyrophosphate and pyrophosphatase enzymes during the zero cycle, increase PCR reaction efficiency through eliminating PCR inhibitor PPi, making it capable to amplify even a trace amount of template DNA.
(a) Inhibition of PCR reaction in the zero cycle
After preparing the PCR mixture, PPi is used to prevent the formation of non-specific reactants before reaching the pre-denaturation temperature.
Also, since DNA polymerases requires Mg ions to activate, PPi, having high affinities with Mg2+ ions, traps them to inhibit the PCR.
PPase included in the mixture is activated when the temperature rises during the denaturation step to decompose PPi into Pi for dissociating the Mg2+ ions.
The isolated ions bind with DNA polymerases to activate, allowing the PCR reaction to proceed.
(c) Annealing & Extension
The activated DNA polymerases amplifies the target DNA as the PCR cycle progresses.
Features and Benefits
Wide choice of real-time PCR instruments for optimized results
Simplified products as all reactants essential for real-time PCR included in each tubes to readily start by adding template DNA, probe & primer for target gene, and D.W.
Enhanced stability having more resistance to degradation than the solution type products with a stabilizer included in the Real-Time PCR reaction mixture
Minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables with mass production under ISO 9001 quality system
• Real-Time Quantification of DNA/cDNA targets.
• Quantification of gene expression
• Microbial/Viral Pathogen Detection.
Figure. Real-Time PCR Data of AccuPower® Greenstar™ qPCR PreMix
AccuPower® Greenstar™ qPCR PreMix provides at least 7 orders of magnitude in dynamic range (10 fg ~ 10 ng/reaction). All data were obtained using Exicycler™ 96 Real-time Quantitative Thermal Block.
(A) : Amplification curve. Lambda DNA primers were added into AccuPower® Greenstar™ qPCR PreMix. A series of Lambda DNA positive control diluents were tested.
(B) : Standard curve. Data were obtained using Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co.).
(C) : Melting curve analysis of AccuPower® Greenstar™ qPCR PreMix. - The melting curve shows that only single amplified PCR product was obtained in all template range.