Protein Synthesis

Protein Synthesis Service

Rapid delivery of purified target proteins using a cell-free expression system

Bioneer provides rapid protein synthesis services using ExiProgen™, a fully automated system capable of expressing and purifying proteins in just 6 hours.

By applying a cell-free protein synthesis system, it is possible to synthesize not only enzymes, antigens, and antibodies, but also toxic proteins that are difficult to produce using cell culture methods.

The entire process, from gene synthesis to protein synthesis, is conducted in a one-stop manner, allowing you to receive custom genes and proteins based solely on genetic information, and verify synthesis results within a single day.

Standard Protein Synthesis Custom Order

Synthesizes and delivers 100 µg of protein within 7–14 days
Protein volume can be scaled up to large size

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One-day Protein Synthesis Custom Order

Provides protein synthesis results within a single day
Synthesized protein delivered immediately

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Gene to Protein Synthesis Custom Order

This service undergoes all the steps from gene synthesis to protein synthesis. N-term His tag & C-term His tag vectors can be selected. For pBT7-C-His vectors, please be aware of the stop codons.
Maximize target protein expression efficiency through GeneCrafter™'s precise AI-based codon optimization.
Design protein variants, recombinations, and de novo proteins that balance thermostability, solubility, and activity Unified Protein Engineering Platform™ (Whitepaper) evaluates every candidate through a 17-stage integrated pipeline. 24 internal modules spanning structure prediction, molecular dynamics, and free energy calculation screen candidates across multiple axes, filtering out designs that will not express or will aggregate before you pay to synthesize them. Built-in immunogenicity prediction and freedom-to-operate (FTO) pre-screening reduce the risk that research stage results require rework at commercialization.
A research-use purification whitepaper covering expression host, tag placement, protease choice, polishing, and QC criteria across nine protein classes, from soluble enzymes to membrane proteins, antibodies, and complexes.

Contract Research (CRO) Services

From design to synthesis, analysis, and validation: CRO services tailored to your research objectives: Bioneer offers therapeutic oligonucleotide design, protein-design-led mRNA engineering, and protein engineering tailored to your research objectives. Our proprietary design platforms and collaboration across specialist departments support contract research programs that connect candidate design with synthesis, quality analysis, and experimental validation.

mRNA Engineering

We first design the protein to be expressed, then optimize the mRNA that encodes it: Protein candidates are first designed and selected to meet the research objectives. We then optimize codon usage, secondary structure, and untranslated regions (UTRs) of the encoding mRNA while preserving the selected amino acid sequence. Proprietary tools including GeneCrafter™, IVTDesigner™, and UTRDesigner™ support candidate designs that consider translation efficiency and stability. DNA template preparation, mRNA synthesis, quality analysis, and expression validation can be integrated within the agreed project scope. Depending on the research objectives, circular RNA (circRNA) and self-amplifying RNA design using CircularDesigner™ and SaRNADesigner™ can also be discussed.

Protein candidate design and selection → mRNA sequence, structure, and UTR optimization → DNA template and mRNA synthesis → Quality analysis and expression validation

Codon Optimization · mRNA Structure Optimization · UTR Design · Circular RNA Design · Self-Amplifying RNA Design

Protein Engineering

From variant, recombinant, and de novo protein design to expression, purification, and functional validation: Using the Unified Protein Engineering Platform™, we design protein variants, recombinant proteins, and de novo protein candidates to meet your research objectives. Computational assessment of structure, function, stability, solubility, and expression potential helps prioritize candidates for experimental evaluation. Through integration with the Gene-to-Protein service, projects can combine gene synthesis, protein expression and purification, quality analysis, and functional validation.

Protein Engineering Whitepaper · Research-Use Protein Purification Whitepaper

Consultation and Service Scope: The scope, deliverables, timeline, and pricing for specialist design and CRO services are defined through project-specific consultation. Once the design is finalized, the relevant in-house departments proceed with oligonucleotide synthesis and the supply of research-use-only (RUO) products. Upon request, these services can also be connected with contract research organization (CRO) services, including quality analysis and experimental validation. Close collaboration among our specialized teams enables coordinated support from design and synthesis through analysis and experimental validation, tailored to each customer's objectives and project requirements. For an effective consultation, please include your research objectives, target or available sequence, requested service area, and desired deliverables.

CRO Service Inquiries: Email geneorder@bioneer.co.kr

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Cloning-free Gene to Protein Synthesis Custom Order

Provides genes and proteins in PCR product form within 15 days
Upgraded protein synthesis success rate through Codon optimization

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