One-day Protein Synthesis Service

Bioneer’s One-day Protein Synthesis Service provides synthesis result of recombinant proteins within a single day. This service uses automatic protein synthesis systems (ExiProgen™ Protein Synthesis System) to synthesize various proteins such as enzymes, growth factors, hormones, and antigen proteins.

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Cat. No.

S-2600

Product Overview

Saving Time with Bioneer's One-day Protein Synthesis Service

Features and Benefits

Rapid production
Protein expression within at least just 1 week using E. coli cell-free protein expression and Ni-affinity purification.
Advanced expression technologies

Expression of proteins (proteins enzyme, growth factor, hormone, antigen protein, antibody, toxic proteins, etc.) difficult to be done in the existing in vivo technology

Workflow

▶ Example of protein synthesis

1 Enzyme for scientific research

* RNase H (Ribonuclease H),
  DUSP3(Dual specific phospatase 3),
  CAT (Chloramphenicol acetyl transferase),
  MMLV RTase(M-MLV Reverse Transcriptase)

* Calml3 (Calmoduline like protein),
  hGH (Human Growth Hormone),
  FGF2 (Fibroblast growth factor 2),
  hLIF (Leukemia inhibitory factor)

Literature/Support

◈ MSDS

• One-day Protein Synthesis Service MSDS

◈ Brochure

• All about Bioneer's Custom Services

◈ Quality Assurance

Bioneer is the holder of Quality Management Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

Amount of sample	E. coli expression vector¹⁾	10 µg over (A_260/280 : 1.7~2.0, A_260/230 : >1.5)
Cost of synthesis	$300
	Note) If protein synthesis fails, you will pay only set-up charge ($100).
Delivery form²⁾	1. Data report – E-mail 2. Protein - 0.1~1.0 mg/ml of solution
Period of synthesis³⁾	1 day

¹⁾ If amount and purity of plasmid DNA are inconsistent with sample condition, an increase in quantity service of DNA will proceed and additional cost will be incurred.
²⁾ We will provide synthesized protein from once a reaction. If you want more protein, the service can be converted into "Standard Protein synthesis Service"
³⁾ Shipping period of sample is excluded from the period.

▶ Quotation and order process

1 Download the order sheet from the link on web site.

2 Fill out the form and email the form to: export1@bioneer.com

* Please fill in the personal information columns (contact information). We need it to fine-tune the service and expedite the order.

3 We will review the order and send quote to you via email.

4 If you decide to go ahead with the service, please send the DNA sample to Bioneer headquarters.

Address: 8-11 Munpyeongseoro, Daedeok-gu, Daejeon 306-220, Republic of Korea.

▶ Consultation Time

Contact
sales@bioneer.co.kr (Tel. +82-42-930-8777)
proteinorder@bioneer.com
Weekdays from 9:00 am to 6:00 pm (closed on weekends and holidays)

FAQ

Question1 How can I order protein synthesis service?

After filling and sending the order form according [Quotation and order process] on the Ordering Information page, please send the template DNA samples according to the conditions below.

DNA type	*E. coli* expression vector (ex) pET etc T7-based vetor)
DNA size¹⁾	0.3 kb ~ 4.0 kb
DNA amount	10 ug or more (A_260/280 : > 1.7, A_260/230 : >1.5)

¹⁾ DNA size represents the size of the ORF (Open Reading Frame) except the vector..

Question2 What is the available protein size for your service?

Our company has synthesis data for molecular weights of 10~150 kDa.

If your requested protein is out of range, a fee of $100 will be charged as additional experiments will be required,

Question3 If I do not have the template DNA, is this service unavailable for me?

If you don’t have the template DNA for your desired proteins, you may try using Gene to Protein service which not only synthesizes genes, but also provide protein synthesis service.

In this case, you must pay an additional cost for the gene expression.

Please refer to Gene to Protein Service order page for more details.

Question4What is the composition of the buffer containing the protein sample? What if I want to change it?

The protein storage buffer composition is as the following:

50 mM Tris-Cl (pH 7.6), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.05% NaN₃, 50% Glycerol.

If you want to recieve your proteins in a specific buffer, please state its name in the order form. However, please note that additional time and costs will be required.

Question5 What kit is used for protein synthesis?

Refer to the table below.

Protein Type	Cat. No.	Product Description
Normal Protein	K-7300	ExiProgen EC Protein Synthesis Kit