ToxinCleanic Endotoxin Removal Kit

ToxinCleanic Endotoxin Removal Kit can be used with spin or gravity column method which may be used up to five times. This kit allows quick removal of endotoxin (2,000,000 EU per 1 ml of resin; 1 EU = 0.1 ng ) from protein with ease.

※This product is shipped with ice pack.

$360.00

Cat. No.

K-7800

Product Overview

Overview

Lipopolysaccharide (LPS) is well known as one of endotoxin. LPS consist of lipid and polysaccharide, which forms outer wall of gram negative bacteria. LPS stimulates immune cells to induce inflammation. It is constantly excreted during cellular growth to cause environmental pollution. Removal of LPS is known to be rigorous process. It can only be removed at temperature of 200℃ or exposure to 0.1 M of strong acid/alkaline for several hours. This kit can easily remove maximum of 2,000,000 EU endotoxins (1EU = 0.1 ng) per 1 ml of resin from protein samples.

Feature and Benefit

Convenience
Easy removal of endotoxins from protein samples just by letting them pass through the resins
Stability
Reusable up to five times
High binding capacity
Remove maximum of 90% (≥ 2,000,000 EU/ml of resin) endotoxins from samples

Application

• Sample purification

• Antibody/immune related research

• Cellular experiment

Technical/Specs

▶ Component

Component	Specification	Storage temp.
ToxinCleanic Endotoxin Removal Resin	10 ml X 1 ea (50% slurry in 20% EtOH)	2 - 8℃
10X Regeneration Buffer	10 ml X 1 ea	2 - 8℃
Manual	1 ea	-

▶ Specification

Category	Details
Matrix	Agarose resin
Volume	10 ml (50% slurry in 20% EtOH)
Min. Binding Capacity	> 2,000,000 EU/ml of resin
Storage and Stability	Resin and buffer can be stored at 2 - 8°C until the expiration date. Do not freeze or vortex vigorously.

▶ Experimental data

Figure 1. FITC fluorescence values are measured for each fractions of LPS-FITC conjugation samples

(Input: LPS-FITC 10⁶ EU/ml - 2 ml, Fraction volume - 1 ml, Resin volume: 1 ml (50% slurry), Column volume - 1 ml).

The analysis of the flow fraction shows that the fluorescent of LPS conjugated with FITC (fluorescent dye) has significantly decreased over 90% comparing the measurements taken before and after the flow through.

Figure 2. Concentration of collected IgG for each fraction

(Input: IgG 500 ug/1 ml, Flow fraction volume: 1 ml, Column volume: 1 ml)

The analysis of the flow fraction shows that the most of Human IgG concentration (≥ 85%) has been recovered. (280 nm Abs. is used to measure concentration of IgG)

Literature/Support