AccuRapid™ TA Cloning kit (20 reactions)

AccuRapid™ TA cloning Kit offers quickly and convenient insertion of Taq-amplified PCR products directly into T-vector with T-overhang on each of its 3’-end.

※This product is shipped in dry ice.

$200.00

Cat. No.

K-7170

Product Overview

Overview

TA Cloning is efficient methods for the cloning of 3’ dA-tailed PCR products generated with Taq DNA polymerase. The PCR products and primers can be used directly without any restriction sites or modifications, and the restriction enzymes are not necessary. AccuRapid™ TA cloning Kit offers quickly and convenient insertion of Taq-amplified PCR products directly into T-vector with T-overhang on each of its 3’-end.

Features and Benefits

Fast & Efficient cloning

15-minute reaction, enables cloning of various size of insert DNA.
Blue/white screening
Provides blue/white selection function through LacZα gene
Convenient exision of inserts
Easy exision of inserts using BamH I with a single restriction digestion
Convenient sequencing
M13 primer sites for sequencing

Technical/Specs

▶ Components

Components	Concentration	Volumes
pBHA-T vector	25 ng/μl	40 μl
Control Insert	70 ng/μl	20 μl
AccuRapid™ 2X Reaction Buffer	-	100 μl
T4 DNA Ligase	200 U/μl	20 μl
Storage Conditions: -20^oC

▶ Vector map

▶ Experimental data

Figure 1. Comparison of clones harboring correct insert after TA Cloning between AccuRapid™ TA cloning kit and other supplier’s TA cloning kit.

The AccuRapid™ TA cloning system gave a high number of recombinants clone across a broad range of insert sizes (500bp – 2,000bp).

Figure 2. The restriction enzyme digestion of recombinant plasmids which obtained by AccuRapid™ TA Cloning kit.

M: 1kb DNA ladder
1 : pBHA-T vector
2 : Insert 1 (500 bp)
3 : Insert 2 (1,000 bp)
4 : Insert 3 (2,000 bp)
5 : Digested with BamH I (pBHA-T haboring Insert 1)
6 : Digested with BamH I (pBHA-T harboring Insert 2)
7 : Digested with BamH I (pBHA-T harboring Insert 3)
The AccuRapid™ TA Cloning kit provides a versatile polylinker with flanking BamH I sites for easy excision of inserts