AccuPrep® Nano-Plus Plasmid Mini Extraction Kit (200 reactions)

AccuPrep^® Nano-Plus Plasmid Extraction Kit uses the alkaline lysis method applied with innovative cell debris and protein removal technology to effectively reduce the time for plasmid DNA extraction.

Extraction time: Mini prep (10 min), Midi prep (40 min), Maxi prep (60 min)

Spin column type

Inquire

Cat. No.

K-3111G

Product Overview

Features and Benefits

Effective purification
A new concept of plasmid DNA extraction kit applied with Bioneer's patented technology to effectively remove cell debris particles and proteins
Rapid high-quality, high-yield productivity
Purification only within 10 minutes.
Comprehensiveness
A wide range of isolation from high- to low-copy number plasmid
High binding capacity
Up to 30 µg using silica based DNA binding column
Rich contents
Endonuclease A Denaturation Buffer (PB Buffer) for endA+ strain

Application

• Gene cloning

• Sequencing

• Transformation

• Transfection

• in-vitro transcription / translation

Technical/Specs

▶ Specifications

Feature	Specification
Starting culture volume	1 - 10 ml
Preparation time	< 10 min
Elution volume	50 - 100 μl
Expected DNA yield*	Up to 20 μg
Expected DNA purity	A_260/280 > 1.8

^* Differences in the quantified values may occur depending on the types of plasmids.

▶ Components

Components	K-3111G
PNA1 Buffer (Resuspension)	60 ml X 1 ea
P2 Buffer (Lysis)	60 ml X 1 ea
PA3 Buffer (Neutralization)	80 ml X 1 ea
PB Buffer (Endonuclease A denaturation)	75 ml X 1 ea
WB2 Buffer (Washing)	16 ml X 2 ea
EA Buffer (Elution)	25 ml X 1 ea
BSTB Solution	36 ml X 1 ea
RNase A Powder, lyophilized	6 mg X 1 ea
AccuPrep^® Binding Column-II plus	200 ea
Collection Tube (for filtration)	200 ea
User's Guide	1 ea

▶ Procedure

▶ Experimental data

accupower

Figure 1. Reduced total prep. time

The centrifugation time for obtaining cleared lysate (the solution containing plasmid DNA) by PNA1 Buffer (Resuspension) of this product has been shortened from traditional 10 minutes to just 1 minute thanks to the nanoparticles inside the solution. AccuPrep^® Nano-Plus Plasmid Mini Extraction Kit allows the extraction of plasmid DNA on mini-scale (up to 20 ug) only within 10 minutes.

- B; AccuPrep^® Nano-Plus Plasmid Mini Extraction Kit
- Q, P; Competitors

Figure 2. Improved prep. yield
AccuPrep^®Nano-Plus Plasmid Mini Extraction Kit is capable of providing high yields of both high- and low-copy number of highly pure plasmid DNA.

- B; AccuPrep^® Nano-Plus Plasmid Mini Extraction Kit
- P, Q; Competitors

Figure 3. Restriction enzyme digestion test

• Plasmid DNA isolated with AccuPrep^® Nano-Plus Plasmid Mini Extraction Kit and a competitors' kit was verified and compared using agarose gel electrophoresis after restriction enzyme digestions.

- pBS; pBluescript SK(+) with XbaI
- pBI121; pBI121 with PstI
- Lane M; Molecular marker (λ-DNA/HindIII + EcoRI, Cat. No. D-1070, Bioneer)
- Lane B; AccuPrep^® Nano-Plus Plasmid Mini Extraction Kit
- Lane Q, P; Competitors

• Plasmid DNA isolated with AccuPrep^® Nano-Plus Plasmid Mini Extraction Kit was verfied using agarose gel electrophoresis after digesting with various restriction enzymes.

- Left; pBluescript SK(+)
- Right; pBI121
- Lane M; Molecular marker (Cat. No. D-1040, Bioneer)

Literature/Support