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With great expertise and experience on molecular biological techniques, Bioneer can provide a simple, straightforward solution to your problems on cloning and sequencing problems.
Features and Benefits
Efficient
Save time and money through manufacturing by integrated production system from genetic raw material to high-speed oligo synthesizer
Accurate quality
100% guaranteed exact sequences using Automatic DNA sequencer (ABI 3730)
Comprehensiveness
Wide range of choices for compatible vectors according to the desired applications at a reasonable price and time
$250.00 / cloning (~ 1kb insert, ~7kb vector) *Cloning is possible for Vector provided by Bioneer.
Additional price
Insert
$50.00 / 500bp(When more than 1,001 bp insert is requested)
Vector
7~10Kb: Add $100.00
10 kb~: inquire
Period required
1~8 kb(vector+insert)
Average 5~10 working days
8~11 kb(vector+insert)
Average 10~15 working days
11 kb~(vector+insert)
inquire
Additional service
Plasmid Increase Service ($100.00/100μg) *Excluding low copy plasmid service
When combined with gene synthesis ($100.00 discount)
* The price and duration can increase depending on the structure and characteristics of the gene. * If you request to purchase a commercial vector for the cloning service, the vector cost will be charged independently. * If template DNA is required, please send 150-200 ng / ul, vol. vol.10ul or more samples in the form of plasmid DNA or 50 ng / μl, vol. 10 μl or more in the form of purified PCR products * Sending the wrong sample will charge a fee of 50% of the total cost, so please check before the packaging. * Canceling the order will charge 50% of total cost * If the customer must halt the service due to personal reasons, we can do so for a maximum of 1 month. Afterwards, order will be canceled automatically with 50% charge of the total cost. * If the sample is PCR product, we do not guarantee 100% reference sequence information.
TA Cloning Service
Available Materials
Purified A-tailing PCR product (50 ng/μl, vol.10 μl or more)
Service price
$99.00/cloning (~ 1 kb insert)
4 sequencing data of different candidates, No sample
Vector: pBHA-T vector (2,931 bp)
Additional price
$50.00/500bp (more than 1,001 bp insert)
Period required
Average 5~10 working days
Additional service
Provide additional sample
$30.00/1 clone (plasmid 2~5 μg)
Rescreening (4 more sequencing data of the candidates)
$50.00/cloning (~1 kb insert)
Plasmid Increase Service
$100.00/100 μg
▶ How to send Template DNA
• Write the agency and client name clearly on the box. Then send your samples to the following address:
• 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea BIONEER CO. Synthetic Biology Team (Tel: +82-42-930-8793, 8515)
▶ Payment Information
• Payment Inquiry: +82-42-930-8777 (Customer Support Center)
▶ Consultation
• Service consultation Tel + 82-1588-9788 (Customer Support Center), + 82-42-930-8793, 8515 (Synthetic Biology Team), or email geneorder@bioneer.co.kr
• Consultation time: Weekdays from 9:00 am to 6:00 pm, UTC +09:00 (closed on weekends and holidays)
All our services can be ordered online in our homepage.
After selecting the service and entering the required information, you can check the quotation for the cost. Your order will be completed upon the payment.
Samples must be least 10 μl with DNA concentration of 100 to 200 ng / μl. Sending the wrong sample will charge 50% of the total price. Please check again before the packaging.
Fill out the name of your agency and client on the box and send the sample to the following address.
8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea BIONEER CO. Synthetic Biology Team (Tel: +82-42-930-8793, 8515)
Cloning service will take an average of 1~2 weeks (5 ~ 10 working days). We cannot guarantee the specific dates for the finalization as many of the samples are difficult to be cloned due to their characteristics. If we fail to do so for two weeks, we will inform you that the cloning is impossible and charge only the 50% of the total payment.
Yes, both are possible. However, while we offer directional cloning service for free(excluding TA Cloning), a fee of $100 will be charged per additional 100 µg of vector, requiring five more days for completion.
The T-vector used for TA Cloning is the pBHA-T vector, a component of AccuRapid™ TA Cloning Kit (K-7170, Bioneer).
In the TA Cloning Service, Insert DNA is inserted into the part marked PCR product in the vector map below.
Yes, but you must select "Yes" in the "Gene Synthesis Product receipt" when ordering the after-synthesis service.
Otherwise, if you press "No," only the final products will be shipped.
Yes, include your order information and gene name and email to geneorder@bioneer.co.kr. We will send it after confirmation.
DNA will be shipped in lyophilized form. Completely dissolve by adding 20 μl of DW or TE buffer (Final 100 ~ 250 ng / μl)..
In lyophilized form, this product can be stored at the room temperature However, for the long-time storage in dissolved state, it must be done frozen at -20°C.
