Cloning Custom Order

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Services related to gene synthesis require some time to ship. Please order separately from the other products.
Only orders before 16:00 KST will start on the same day. (If we need to receive your samples, it will start on the date of their arrival.)
COVID-19 / Inf A, B related gene synthesis and cloning service are not available.

Gene Synthesis Service [Order Notice]

Sequence Name

5' Add Sequence

3' Add Sequence

Sequence

Mutant Name

Mutant Sequence

Mutant Position [help]

Protein

Species

Library [help]

AccuGene Block Scale

End Type

Gene Synthesis Vector

Rapid Gene Synthesis Vector

Mutagenesis Vector

Codon Library Mutagenesis Vector

Gene to Protein Vector

Insert Type

Insert Size

Cloning Vector Name

Cloning Vector Size

Customer provided Vector

Cloning Vector Sequence

Product

Antibiotics Name

5' Enzyme Name

3' Enzyme Name

Forward Sequencing Primer Name

Reverse Sequencing Primer Name

Toxic

Gene Synthesis E-coli Stock Service

Gene Synthesis Plasmid Increase Service

Rapid Gene E-coli Stock Service

Rapid Gene Plasmid Increase Service

Mutant E-coli Stock Service

Mutant Plasmid Increase Service

Cloning E-coli Stock Service

Cloning Plasmid Increase Service

Codon Library E-coli Stock Service

Codon Library Plasmid Increase Service

His tag position

Disulfide bond

Storage buffer [help]

Gene synthesis product receipt [help]

Mutagenesis product receipt [help]

Codon Library Mutagenesis product receipt [help]

Comments

Gene Synthesis Invoice Information

Qty

Product Overview

overview

With great expertise and experience on molecular biological techniques, Bioneer can provide a simple, straightforward solution to your problems on cloning and sequencing problems.

Features and Benefits

Efficient

Save time and money through manufacturing by integrated production system from genetic raw material to high-speed oligo synthesizer
Accurate quality

100% guaranteed exact sequences using Automatic DNA sequencer (ABI 3730)
Comprehensiveness
Wide range of choices for compatible vectors according to the desired applications at a reasonable price and time

Workflow

1Online quotation confirmation and order

Check the quotation online.

After confirming it, add to the shopping cart and finalize your order.

2Oligo synthesis

Design oligo for gene synthesis and cloning.

3Cloning

Assemble oligo fragments and clone it into the appropriate vector.

4Screening

Select candidates and amplify the vector.

5Sequencing

Process sequencing step with guaranteed accuracy of cloned genes

6Data analysis

Proceed quality control tests based on sequencing data.

7Synthetic gene delivery

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

Gene Cloning Service
Available materials	Plasmid, PCR product
Service price	$200.00 / cloning (~ 1kb insert, ~7kb vector) *Cloning is possible for Vector provided by Bioneer.
Additional price	When using Cultured Cell	Add $50.00
	Insert	$50.00 / 500bp(When more than 1kb insert is requested)
	Vector	7~10Kb: Add $100.00
		10 kb~: inquire
Period required	1~8 kb(vector+insert)	Average 5~10 working days
	8~11 kb(vector+insert)	Average 10~15 working days
	11 kb~(vector+insert)	inquire
Additional service	Plasmid Increase Service ($100.00/100μg) *Excluding low copy plasmid service
	E. coli Cell stock supply service ($100.00)
	When combined with gene synthesis ($100.00 discount)

* The price and duration can increase depending on the structure and characteristics of the gene.
* If you request to purchase a commercial vector for the cloning service, the vector cost will be charged independently.
* If template DNA is required, please send 150-200 ng / ul, vol. vol.10ul or more samples in the form of plasmid DNA or 50 ng / μl, vol. 10 μl or more in the form of purified PCR products
* Sending the wrong sample will charge a fee of 50% of the total cost, so please check before the packaging.
* Canceling the order will charge 50% of total cost
* If the customer must halt the service due to personal reasons, we can do so for a maximum of 1 month. Afterwards, order will be canceled automatically with 50% charge of the total cost.

▶ How to send Template DNA

• Write the agency and client name clearly on the box. Then send your samples to the following address:

• 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea BIONEER CO. Synthetic Biology Team (Tel: +82-42-930-8793, 8515)

▶ Payment Information

• Payment Inquiry: +82-42-930-8777 (Customer Support Center)

▶ Consultation

• Service consultation
Tel + 82-1588-9788 (Customer Support Center), + 82-42-930-8793, 8515 (Synthetic Biology Team), or email geneorder@bioneer.co.kr

• Consultation time: Weekdays from 9:00 am to 6:00 pm, UTC +09:00 (closed on weekends and holidays)

FAQ

Question1How can I use this service?

All our services can be ordered online in our homepage.

After selecting the service and entering the required information, you can check the quotation for the cost. Your order will be completed upon the payment.

If you need additional information, send an email to geneorder@bioneer.co.kr

Question2How should I send my samples?

Samples must be least 10 μl with DNA concentration of 100 to 200 ng / μl. Sending the wrong sample will charge 50% of the total price. Please check again before the packaging.

Fill out the name of your agency and client on the box and send the sample to the following address.

8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea BIONEER CO. Synthetic Biology Team (Tel: +82-42-930-8793, 8515)

Question3How long will the service take?

Cloning service will take an average of 1~2 weeks (5 ~ 10 working days). We cannot guarantee the specific dates for the finalization as many of the samples are difficult to be cloned due to their characteristics. If we fail to do so for two weeks, we will inform you that the cloning is impossible and charge only the 50% of the total payment.

Question4Are plasmid amplification and direction cloning also avaliable in this service?

Yes, both are possible. However, while we offer directional cloning service for free, a fee of $100 will be charged per additional 100 µg of vector, requiring five more days for completion.

Question5If I choose after-synthesis service, is it also possible to recieve the synthesized products?

Yes, but you must select "Yes" in the "Gene Synthesis Product receipt" when ordering the after-synthesis service.

Otherwise, if you press "No," only the final products will be shipped.

Question6May I request for the sequence data and the sheet from the file?

Yes, include your order information and gene name and email to geneorder@bioneer.co.kr. We will send it after confirmation.

Question7How do I use finished products?

DNA will be shipped in lyophilized form. Completely dissolve by adding 20 μl of DW or TE buffer (Final 100 ~ 250 ng / μl)..

In lyophilized form, this product can be stored at the room temperature However, for the long-time storage in dissolved state, it must be done frozen at -20°C.