AccuPower® GoldHotStart Taq PCR PreMix applies GoldHotStart Taq DNA polymerase to not only enhance specificity and PCR amplification, but also reduce non-specific reactions such as mis-priming and primer-dimer formation that may occur at a low temperature. In addition, the components necessary for PCR such as DNA polymerases, dNTPs, reaction buffers, etc. are thoroughly mixed and vacuum-dried, each packaged with amounts sufficient for a single PCR run.
※ Master Mix product is shipped in dry ice
Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer.
※PreMix product is shipped at room temperature. The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
※MasterMix product is shipped in dry ice.
Minimized generation of non-specific amplification products in the zero cycle by GoldHotStart Taq DNA polymerase and accurate amplification of correct target seqences by activated HotStart Taq polymerase
Excellent sensitivity and amplification efficiency with mimimized non-specific reactions by detecting even a trace amount of samples
Simplified procedure by requiring only the addition of template DNA, primer sets, and D.W., with all necessary components and DNA polymerases included and mixed in each of the tubes packaged with sufficient amount for 1 PCR run, along with tracking dye and sedimentation agents also included for electrophoresis not requiring the addition of sample loading buffer
Enhanced stability to maintain stable enzymatic activities for long-term storage by including stabilizer and dried in the PCR reaction mixture
Comprehensive compatible types of samples for amplification such as gDNA templates, low-copy targets, etc.
Reproducible experimental results by mass production in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product
• High specificity PCR
• High sensitivity PCR
• gDNA template PCR
• Low-copy target PCR
• Multiple primer pairs PCR
• cDNA template PCR
• TA cloning
Figure. Comparison of PCR amplification efficiency between AccuPower® GoldHotStart Taq PCR PreMix from Bioneer and other supplier’s Hotstart PCR master mix.
The cycling conditions for AccuPower® GoldHotStart Taq PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 30 sec, 55°C for 30 sec and 72°C for 30 sec. PCR reactions using other supplier’s PCR master mix were performed according to each supplier's protocol.
Target : human insulin receptor gene.
Lane M; 100 bp DNA Ladder(Cat. No. D-1030, Bioneer)
Lane 1; 10 ng of human genomic DNA
Lane 2; 1 ng of human genomic DNA
Lane 3; 100 pg of human genomic DNA
Lane 4; 10 pg of human genomic DNA