M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase capable of using an RNA molecule as a template to synthesize a double-stranded DNA. It is ideal for use of first-strand synthesis cDNA for Reverse Transcriptase PCR and qRT-PCR

※This product is shipped in dry ice.

As low as $72.00

Cat. No.

Product Overview

Features and Benefits

Optimized buffer delivery
Guarenteed reliable response by providing optimized for M-MLV reverse transcriptase
Reproducibility
Reproducible results with uniform quality products for each batch under the ISO 9001 quality system

Application

First strand cDNA synthesis from RNA, RT-PCR, and qRT-PCR

Technical/Specs

▶ Specifications

5' to 3' exonuclease activity	No
3' to 5' exonuclease activity	No
3' – A overhang	No
Strand displacement	Yes
Fragment size	Up to 9 kb

▶ Components

5X reaction buffer: Tris-HCl, KCl, MgCl₂(pH 8.1)

100 mM DTT

10 mM dNTPs mix: 2.5 mM of each dNTP

▶ Concentration

10,000 Units (200 U/μl)

▶ Storage conditions

50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50 mM (NH₄)₂SO₄, Stabilizers.

▶ Storage temperature

-20°C

▶ Unit Definition

One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A) oligo (dT) as template primer.

Literature/Support

◈ Manual

• Manual_MMLV Reverse Transcriptase

◈ MSDS

• MSDS_10 mM dNTPs

• MSDS_100 mM DTT

• MSDS_5X Reaction buffer for M-MLV Reverse Transcriptase

• MSDS_M-MLV Reverse Transcriptase

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

Ordering Info

Cat. No.	Product Description	Option	Price
E-3121	M-MLV Reverse Transcriptase	10,000 U(50 rxn)	$E-3121
E-3122	M-MLV Reverse Transcriptase	50,000 U(250 rxn)	$E-3122