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AccuTarget™ GFP Positive Control siRNA
AccuTarget™ Positive control siRNAs show high efficiency knockdown effects on target genes. It provides efficient siRNA for GAPDH, a widely used Housekeeping gene, and also provides Positive Control siRNA for GFP and Luciferase as a reporter system.
Overview
- AccuTarget ™ PC siRNA (Human GAPDH, GFP, Luciferase): KD effect of highly efficient target gene is verified
- AccuTarget ™ NC siRNA (Human, Mouse, Rat common) : The siRNA sequence has low homology to target genes
- Fluorescently labeled siRNA allows simply to monitor siRNA delivery.
- AccuTarget ™ Control siRNA Sets (PC+ NC): Simple and inexpensive control sets can be purchased together.
- Various scale (5, 10, 20 nmole) and purification (BioRP, HPLC) can be selected.
Features and Benefits
- HIgh Efficiency
Positive control siRNA with knockdown efficiency being over 90%.
- Convenient monitoring
Easy validation for transfection rate of negative control set by the use of fluorescent labels
- Financial price
Maximized efficiency considering the research cost
▶ Experimental data
• Positive and Negative Control siRNA
A) Northern Blotting | B) qRT-PCR |
Figure 1. Effects of Human GAPDH Positive Control siRNA.
HeLa cells were transfected separately with AccuTarget ™ Human GAPDH Positive Control and Negative Control siRNA using Lipofectamine 2000 (Invitrogen) at a final concentration of 100 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to northern blot and real-time PCR analysis. As can be seen from Fig.1-B, about 3% GAPDH mRNA remained.
• GFP Control siRNA
Figure 2. HeLa cells in a 24-well plate were cotransfected with 200 ng of CMV-GFP plasmid and 10 nM of GFP siRNA using Lipofectamine 2000 transfection reagent. Next day, the expression of GFP was observed by using a Nikon Eclipse TS100 epifluorescence microscope. In contrast to bright green fluorescence of GFP protein in NC-siRNA-transfected cells, no fluorescence was detected from GFP-siRNA-transfected cells, indicating efficient knockdown of GFP by using our positive control GFP-siRNA.
• Luciferase Control siRNA
Figure 3. HeLa cells in a 6-well plate were cotransfected with 400 ng of CMV-luc plasmid and 10 nM of luciferase siRNA using lipofectamine 2000 transfection reagent. Next day, cells were harvested and assayed for luciferase activity. As shown in Fig. 3, cotransfection with our positive control luciferase siRNA led to efficient knockdown of luciferase activity (85% - 95% knockdown compared to luciferase activity of NC-siRNA-transfected cells).
• Mouse Positive Control siRNA
Figure 4. Effects of Mouse Lamin A/C Positive Control siRNA.
Hepa1-6 cells were transfected separately with AccuTarget ™ Mouse Lamin A/C Positive Control and Negative Control siRNA using Lipofectamine RNAimax (Invitrogen) at a final concentration of 1 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to real-time PCR analysis.
Figure 5. Effects of Mouse Ppib (Cyclophilin B) Positive Control siRNA.
Hepa1-6 & NIH 3T3 cells were transfected separately with AccuTarget ™ Mouse Ppib (Cyclophilin B) Positive Control and Negative Control siRNA using Lipofectamine RNAimax (Invitrogen) at a final concentration of 5 & 1 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to real-time PCR analysis.
◈ Manual
• AccuTarget™ Genome-wide Predesigned siRNA library
• Annealing of single-stranded RNA for siRNA
• AccuTarget™ Real-Time PCR Primer Library
◈ Brochure
• All about Bioneer's Custom Services
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
▶ AccuTarget ™ Positive Control siRNAs
Cat. No. | Product Description | Purification | Guaranteed Yield | Price |
SP-1001 | AccuTarget ™ GAPDH Positive Control siRNA | BioRP | 5 nmole | $SP-1001 |
SP-1002 | 10 nmole | $SP-1002 | ||
SP-1003 | 20 nmole | $SP-1003 | ||
SP-1011 | HPLC | 5 nmole | $SP-1011 | |
SP-1012 | 10 nmole | $SP-1012 | ||
SP-1013 | 20 nmole | $SP-1013 | ||
SP-2001 | AccuTarget ™ GFP Positive Control siRNA | BioRP | 5 nmole | $SP-2001 |
SP-2002 | 10 nmole | $SP-2002 | ||
SP-2003 | 20 nmole | $SP-2003 | ||
SP-2011 | HPLC | 5 nmole | $SP-2011 | |
SP-2012 | 10 nmole | $SP-2012 | ||
SP-2013 | 20 nmole | $SP-2013 | ||
SP-3001 | AccuTarget ™ Luciferase Positive Control siRNA | BioRP | 5 nmole | $SP-3001 |
SP-3002 | 10 nmole | $SP-3002 | ||
SP-3003 | 20 nmole | $SP-3003 | ||
SP-3011 | HPLC | 5 nmole | $SP-3011 | |
SP-3012 | 10 nmole | $SP-3012 | ||
SP-3013 | 20 nmole | $SP-3013 | ||
SP-4001 | AccuTarget ™ Mouse Lamin A/C Positive Control | BioRP | 5nmole | $SP-4001 |
SP-4002 | 10nmole | $SP-4002 | ||
SP-4003 | 20nmole | $SP-4003 | ||
SP-4011 | HPLC | 5nmole | $SP-4011 | |
SP-4012 | 10nmole | $SP-4012 | ||
SP-4013 | 20nmole | $SP-4013 | ||
SP-5001 | AccuTarget ™ Mouse cyclophilin B c Positive Control | BioRP | 5nmole | $SP-5001 |
SP-5002 | 10nmole | $SP-5002 | ||
SP-5003 | 20nmole | $SP-5003 | ||
SP-5011 | HPLC | 5nmole | $SP-5011 | |
SP-5012 | 10nmole | $SP-5012 | ||
SP-5013 | 20nmole | $SP-5013 |
▶ AccuTarget ™ Negative Control siRNAs
Cat. No. | Product Description | Purification | Guaranteed Yield | Price |
SN-1001 | AccuTarget ™ Negative Control siRNA | BioRP | 5 nmole | $SN-1001 |
SN-1002 | 10 nmole | $SN-1002 | ||
SN-1003 | 20 nmole | $SN-1003 | ||
SN-1011 | HPLC | 5 nmole | $SN-1011 | |
SN-1012 | 10 nmole | $SN-1012 | ||
SN-1013 | 20 nmole | $SN-1013 | ||
SN-1021 | AccuTarget ™ Fluorescein-labeled Negative Control siRNA | HPLC | 5 nmole | $SN-1021 |
SN-1022 | 10 nmole | $SN-1022 | ||
SN-1023 | 20 nmole | $SN-1023 |
▶ AccuTarget ™ Control siRNA Sets
Cat. No. | Product Description | Guaranteed Yield | Purification | Target | Price |
SS-1001 | AccuTarget ™ GAPDH Control siRNA Set | 5 nmole positive control + 2 nmole negative control |
BioRP | GAPDH | $SS-1001 |
SS-1002 | GFP | $SS-1002 | |||
SS-1003 | Luciferase | $SS-1003 | |||
SS-1011 | HPLC | GAPDH | $SS-1011 | ||
SS-1012 | GFP | $SS-1012 | |||
SS-1013 | Luciferase | $SS-1013 |