S. pombe Validation Primer set ver 3.0 to ver 4.0 Upgrade Pkg (278 primers)
This product consists of 2 sets of 278 primers. It is used to confirm the quality of S. pombe haploid deletion mutants ver 3.0 to ver 4.0 Upgrade Pkg by PCR. It consists of mixture of a specific primer for the target gene and a common primer for the KanMX module, a deletion cassette. so you can easily prepare PCR mixture by one pipetting.
Features and Benefits
Deletion mutants of each S.pombe gene
Similar physiological process with mammalian cells
Human cancer-related genes with over 30% of homology
Rapid cell cycle(~3h) with simplified analysis of molecular biological mechanism and pathway
Phenotype analysis possible with it's recessive mutant type
Unknown genomic function analysis through functional complementation
Drug target screening at the genomic level in living cells available
Schizosaccharomyces pombe (S. pombe) Genome-wide Deletion Mutant Library was developed in collaboration with Bioneer, Korea Research Institute of Bioscience and Biotechnology (KRIBB) and Cancer Research UK. However, Bioneer is the only institute with exclusive business license. It is a powerful research tool for scale gene function analysis, drug target identification, validation studies, and global cell function studies. It can also be used for gene function analysis and drug screening, such as gene expression and synthetic lethal analysis. Schizosaccharomyces pombe has higher gene homology with mammals including humans than germinated yeast. Furthermore, cell division of Schizosaccharomyces pombe is similar to that of mammals. Therefore, it is considered to be a more ideal model than other animals. Thus, Schizosaccharomyces pombe (S. pombe) Genome-wide Deletion Mutant Library was produced by homologous recombination, replacing the target gene with a selection cassette containing KanMX4.
- The Heterozygous diploid deletion mutants library consists of a total of 4,845 variants, accounting for approximately 98% of the total genome (including 4,914 total ORFs).
- The Homozygous diploid deletion mutants library consists of a total of 3,420 variants.
Each strain has a specific barcode on both sides of the selection marker (KanMX4), which provides an easy means of analyzing large amounts of gene function and drug target screening in the entire strain pool. In addition, by analyzing the expression traits of each individual strain, it can be used effectively to identify the function of genes.
Application #1 (Anti-fungal)
Figure 1. Diagrams showing a new discovered pmm1 gene as a new target of terbinafine for eliminating side effects by treating the antibiotic terbinafine in the whole library
Figure 2. A diagram showing the sensitivity of S. pombe system to terbinafine being 1000-times more than that of S. cerevisiae system, also meaning more specificity.
(Pombe gene is more specific for this antimicrobial agent)
Application #2 (Anti-hyperlipidemic)
Figure 3. The diagram showing that HMG1 gene, the action point of simvastatin, a therapeutic agent for the treatment of hyperlipidemia, was isolated from the fission yeast S. pombe and was not detected in the germination yeast S. cerevisiae.
(S. pombe can be found to be more effective in screening for this hyperlipidemic drug)
Application #3 (General toxicity)
Figure 4. Diagrams presenting that a hazardous substance troglitazone was showing toxicity at a concentration of about 10 uM in fission yeast, whille rosiglitazone, a safe drug, was not.
(In the early stage of drug development, general toxicity is evaluated, meaning that decision-making time for drug development will be shortened while the fission yeast system can be effectively used to prevent recalls from side effects.)