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AccuPower® PCR PreMix (96 T, 50 μl)
AccuPower® PCR PreMix uses Bioneer’s patented technology to individually vacuum dry components necessary for PCR such as DNA polymerase, dNTPs, reaction buffer, etc. A chemical stabilizer is also included for retaining the activity of the products for 1 month at a room temperature (25 °C) and over 2 years in a freezer.
※ This product is shipped at room temperature.
※ The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.
Features and Benefits
- Stability
Addition of stabilizing agents allows delivery at room temperature and enhanced stability compared to solution-type products
- User-friendly
Amounts of reactants required for one PCR cycle included in each of the PCR tubes, allowing anyone to perform PCR tests simply by adding template DNA and primers
- Sensitivity
Excellent sensitivity and amplification efficiency even with small amounts of DNA
- Reproducibility
Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variables
Application
• Conventional PCR
• Primer extension
• TA cloning
• Gene sequencing
Experimental data
Figure. Comparison of heat-stability of AccuPower® PCR PreMix
AccuPower® PCR PreMix is incubated at 95℃ with various time.
Kit: AccuPower® PCR PreMix (Batch-1/2)
A; 30 min, B; 60 min, C; 90 min
Lane 1; Human genomic DNA 10 ng
Lane 2; Human genomic DNA 1 ng
Lane 3; Human genomic DNA 100 pg
Lane 4; Human genomic DNA 10 pg
M ;100 bp plus DNA ladder (Bioneer Cat. No: D-1035)