AccuPower® Dual-HotStart™ RT-PCR PreMix (with UDG) is a one-step RT-PCR product that applied Pyro-Hotstart RT technology and Hotstart PCR technology to substantially improve the problems of non-specific reverse reaction and effectively synthesize cDNA even when the template RNA is in secondary structure or is in scarce amounts. Application of Uracil DNA glycosylase systems prevents carryover contaminations. As 1 tube contains sufficient amounts of mixture comprised with components necessary for RT-PCR , not only the process can start convienantly just by adding the template RNA, the primer, and D.W., but the tubes can also be dispensed afterwards, preventing cross-contamination caused by the repeated use of a master mix product.
※This product is shipped in dry ice.
Application of uracil DNA glycosylase system allows prevention of carryover contaminations caused by aerosols containing nucleotides from repetitive PCR reactions. Uracil DNA Glycosylase (UDG) hydrolyzes the N-glycosidic bonds linking uracil and deoxyribose, breaking down the leftover templates inserted with uracil. Before the PCR, activate the enzymes at 37℃ for 2 minutes to remove the DNA aerosols. During the cycles at the higher temperature, they are deactivated.
The world's first Dual-HotStart ™ RT-PCR reaction using Pyro-Hotstart RT reaction and Hotstart PCR optimized to accurately detect only the target gene.
• Low copy viral/bacterial pathogen load determination in an earlier stage
• Low copy mRNA amplification
• Low copy target RNA quantification
• RNA amplification for microarray and NGS
Figure. Sensitivity of AccuPower® Dual-Hotstart ™ RT-PCR PreMix(with UDG).
