EcoQprep™ Genomic DNA Kit

EcoQprep™ Genomic DNA Kit is a magnetic bead–based system for isolating high-quality genomic DNA from cultured cells, animal tissues, and plant tissues. Using specially designed magnetic nanobeads and the EcoQprep™ Magnetic Separation Rack, the kit provides a faster and simpler workflow than conventional spin column methods. Because it does not require centrifugation, it reduces energy consumption and minimizes plastic waste. The purified genomic DNA is ready for downstream applications such as PCR, qPCR, sequencing, genotyping, and cloning.

$130.00

Cat. No.

K-3701

Product Overview

Introduction

Features

Efficient magnetic bead-based extraction
Magnetic particles enable rapid nucleic acid separation with a magnet instead of centrifugation.
Eco-friendly nucleic acid extraction
Reduces plastic waste compared to spin column filters.
Compatible with a wide range of sample types
Extracts high-purity, high-yield genomic DNA from a wide range of samples, including whole blood, cultured cells, animal tissues, Gram-negative and Gram-positive bacteria, and forensic specimens.
Compatible with various scales
Processes Mini, Midi, and Maxi scales with a single kit, allowing for flexible handling of sample volumes ranging from small to large.
Reliable extraction from small sample volumes
Extracts DNA from as few as 1x10⁴cultured cells, making it ideal for processing small samples.
Fast processing time
Using magnetic particle technology, nucleic acid extraction takes approximately 5 minutes* for Mini, 10 minutes* for Midi, and 15 minutes* for Maxi, significantly improving experimental efficiency.
Minimal equipment needed
Only EcoQprep™ Magnetic Separation Rack(TM-1012) is required—no additional equipment needed.

^*Excluding pre-treatment time

Applications

• Gene cloning

• PCR

• Real-time PCR

• Southern blotting

• SNP genotyping

• Short Tandem Repeat (STR) analysis

Technical/Specs

▶ Specifications

Scale		Micro	Mini	Midi	Maxi
Whole blood	Amount of Starting Sample	100 μL	200 μL	2 mL	4 mL
Whole blood	Typical DNA Yield [μg]	< 5 μg	< 10 μg	< 80 μg	< 150 μg
Cultured cells	Amount of Starting Sample	~ 1 x 10⁴ cells	~ 1 x 10⁶ cells	~ 5 x 10⁶ cells	~ 1 x 10⁷ cells
Cultured cells	Typical DNA Yield [μg]	< 120 ng	< 12 μg	< 60 μg	< 120 μg
Animal tissue	Amount of Starting Sample	~ 10 mg	~ 25 mg	~ 100 mg	~ 250 mg
Animal tissue	Typical DNA Yield [μg]	< 5 μg	< 10 μg	< 40 μg	< 120 μg
Bacteria (Gram (-), (+))	Amount of Starting Sample	-	~ 1 x 10⁹ cells	~ 5 x 10⁹ cells	~ 1 x 10¹⁰ cells
Bacteria (Gram (-), (+))	Typical DNA Yield [μg]	-	< 15 μg	< 80 μg	< 150 μg
DNA purity		A_260/280> 1.8
Turnaround Time		> 5 min	> 5 min	> 10 min	> 15 min

Various kinds of forensic sample
Amount of Starting Sample	Dried body fluid spot or fingerprint (FTA card, paper, cloth, etc)	~ 7 mm or ~ 2 cm²
	Hair	1 cm
	Bone and teeth	~ 100 mg
	Chewing gum	~ 30 mg
	Cigarette butts	~ 2 cm²
	Buccal swab	Single piece of swab
	Urine	~ 15 mL
	Saliva	1-100 μL
Typical DNA Yield [μg]	~ 5 μg
DNA purity	A_260/280> 1.8
Turnaround Time	> 10 min

* Measurements may differ by sample type.

▶ Components

Components	Volume	Quantity*
TL Buffer	15 mL	1 ea
GB Buffer	15 mL	1 ea
WM1 Buffer	30 mL	1 ea
WB2 Buffer	10 mL	1 ea
WE Buffer	40 mL	1 ea
EA Buffer	25 mL	1 ea
Proteinase K powder, lyophilized	25 mg	1 ea
RNase A powder, lyophilized	24 mg	1 ea
Poly(A), lyophilized	1 mg	1 ea
Magnetic Nanobead	6 mL	1 ea
1.5 mL Tube	50 ea	1 pack
One Page Protocol	-	1 ea

* Mini-50 rxn, Midi-7 rxn, Maxi-3 rxn

▶ 실험 자료

M: 1 kb ladder (Cat. No. D-1040, Bioneer)
BIONEER: EcoQprep™ Genomic DNA Kit
A: Competitor A Kit

Sample	EcoQprep™		Competitor A
Sample	Yield (μg)	Purity (A_260/280)	Yield (μg)	Purity (A_260/280)
Whole blood (Human blood)	6.45 μg	1.85	5.53 μg	1.85
Animal tissue (porcrine skeletal muscle tissue)	5.48 μg	1.86	5.60 μg	1.85
HeLa Cell	24.1 μg	1.92	23.3 μg	2.00
E. coli	14.7 μg	1.83	15.5 μg	2.05

Figure 1. Comparison of Genomic DNA Extraction from Various Samples using EcoQprep™ Genomic DNA Kit and Competitor’s kit (column type).

(a) Whole blood: DNA extracted from 200 µL
(b) Animal tissue: DNA extracted from 15 mg of porcine skeletal muscle tissue
(c) HeLa cell: DNA extracted from 1x10⁶ cells/rxn
(d) Escherichia coli : DNA extracted from 1x10⁹ cells/rxn (DH5α)

M: 1 kb ladder (Cat. No. D-1040, Bioneer)
Mini, Midi: EcoQprep™ Genomic DNA Kit

Scale	Mini			Midi
Sample	Starting amount	Yield (μg)	Purity (A_260/280)	Starting amount	Yield (μg)	Purity (A_260/280)
Whole blood (Human blood)	100 μL	3 μg	1.80	1 mL	30.85 μg	1.82
Animal tissue (porcrine skeletal muscle tissue)	20 mg	5.48 μg	1.81	100 mg	23.9 μg	1.81
HeLa Cell	1 x 10⁶ cells	13.6 μg	1.83	5 x 10⁶ cells	75.3 μg	1.83
E. coli	1 x 10⁹ cells	14.7 μg	1.83	5 x 10⁹ cells	83.9 μg	1.80

Figure 2. Genomic DNA Extraction from Various Samples using EcoQprep™ Genomic DNA Kit at Mini and Midi scales.

Figure 3. Comparison of real-time qPCR data of genomic DNA from HeLa cell (1 x 10¹ - 1 x 10⁵) purified with EcoQprep™ Genomic DNA Kit and competitor’s kit (single column type).

(a) Amplication curve of GAPDH from HeLa cell (1 x 10¹ - 1 x 10⁵) genomic DNA purified with Bioneer EcoQprep™ Genomic DNA Kit.
The premix used was Accupower^® Plus DualStar™ qPCR PreMix (K-6600).
(b) Comparison of standard curves from qPCR results using genomic DNA purified with Bioneer EcoQprep™ DNA Kit and competitor A kit.

Sample	Whole blood	Saliva	Fingerprint
EcoQprep™	28.54	28.07	36.33
EcoQprep™	28.18	28.43	36.29
Competitor A	28.64	28.1	36.45
Competitor A	29.08	28.59	36.45

Figure 4. Genomic DNA Extraction from Paper Using EcoQprep™ Genomic DNA Kit Compared with a Competitor’s kit (column type).

After DNA extraction, real-time PCR is performed using the AccuPower® Plus DualStar™ qPCR PreMix with GAPDH as the target.
(a) Blood: DNA extracted from a 7 mm diameter punched sample of dried human whole blood (25 µL) on Whatman FTA card.
(b) Saliva: DNA extracted from a 7 mm diameter punched sample of dried human saliva (10 µL) on paper.
(c) Fingerprint: DNA extracted from a 7 mm diameter punched sample of fingerprint traces on paper.

Sample	Whole blood	Saliva
EcoQprep™	29.15	30.30
EcoQprep™	29.14	30.60
Competitor A	29.69	32.05
Competitor A	29.89	32.14

Figure 5. Genomic DNA Extraction from Fabric Using EcoQprep™ Genomic DNA Kit Compared with a Competitor’s kit (column type).

After DNA extraction, real-time PCR is performed using the AccuPower® Plus DualStar™ qPCR PreMix with GAPDH as the target.
(a) Blood: DNA extracted from a 7 mm diameter punched samples of human blood (10 µL) stains on fabric.
(b) Saliva: DNA extracted from a 7 mm diameter punched samples of human saliva (10 µL) stains on fabric.

Scale	Bone	Nail Clipping	Hair (with root)	Hair (without root	Cotton Swab	Chewing Gum
EcoQprep™	29.63	28.00	29.99	33.39	26.41	32.10
EcoQprep™	29.48	27.98	30.04	33.08	26.29	32.25
Competitor A	29.63	30.04	30.7	34.66	26.87	31.21
Competitor A	28.75	29.46	30.4	34.22	26.45	31.10

Figure 6. Genomic DNA Extraction Using EcoQprep™ Genomic DNA Kit Compared with a Competitor’s kit (column type).

After DNA extraction, real-time PCR is performed using the AccuPower® Plus DualStar™ qPCR PreMix with GAPDH as the target.
(a) Bone: DNA extraction from porcine bone 100 mg.
(b) Nail Clipping: DNA extraction from 15 mg of nail clipping.
(c) Hair: DNA extraction from 1 strand of hair with or without root.
(d) Cotton swab: DNA extraction from cotton swab with human saliva.
(e) Chewing Gum: DNA extraction from chewing gum with human saliva.

Literature/Support

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

Ordering Info

▶ Ordering Information

Cat. No.	Product	Option
K-3702	EcoQprep™ Plant Genomic DNA Kit (V1)	50 rxn

▶ Related Products

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