MagListo™ 5M Plant Genomic DNA Extraction Kit (100 reactions)

MagListo™ 5M Plant Genomic DNA Extraction Kit allows extraction of total DNA using Magnetic Nanobeads and MagListo™ Magnetic Separation Rack from various plant samples such as leaves, roots, seeds. The isolated genomic DNA can be used for a wide range of experiments such as gene cloning, PCR, Quantitative real time PCR, southern blotting, etc.

Magnetic bead type

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Cat. No.

K-3605

Product Overview

Features and Benefits

Flexible sample choice
Isolation of highly-pure genomic DNA from plant samples such as leaves, stem, roots, seeds with high-yield
Great lysis optimization
Lysis Buffer included in the kit for various plant seeds and tissue cells
Convenience
Broad coverage of scales for Mini, Midi, and Maxi isolation protocols with just a single kit (Mini - within 5min, Midi - within 10min, Maxi - within 15min)
Cost-effective kit
No need of additional expensive instruments other than the kit and MagListo™ Magnetic Separation Rack

Application

• Gene cloning

• PCR

• Real-time PCR

• Southern blotting

• SNP genotyping

Technical/Specs

▶ Specifications

Scale	Mini (typical yield (μg))	Midi (typical yield (μg))	Maxi (typical yield (μg))
Plant tissue	Up to 100 mg	Up to 500 mg	Up to 1 g
Seed	Up to 50 mg	Up to 250 mg	Up to 500 mg
Elution volume	100 ul	500 ul	1 ml
Typical DNA yield*	5 - 15 ug	15 - 80 ug	30 - 150 ug
Expected purity	A_260/280> 1.7

* Differences in the quantified value may occur depending on the types of samples.

▶ Components

Components	K-3605*
PL Buffer	35 ml x 1 ea
PC Buffer	30 ml x 1 ea
PWM1 Buffer	60 ml x 1 ea
W2 Buffer	80 ml x 1 ea
WE Buffer	120 ml x 1ea
EA Buffer	25 ml x 1 ea
RNase A powder, lyophilized	24 mg x 2 ea
Proteinase K, lyophilized	25 mg x 2 ea
Magnetic Nanobead	11 ml x 1 ea
User's Guide	1 ea

*mini-100 rxn, midi-15 rxn, maxi-8 rxn

▶ Experimental data

Figure 1. Comparison of genomic DNA from plants purified with MagListo™ 5M Plant Genomic DNA Extraction Kit and that done with a competitor’s kit (single column type).

- M; Bioneer 1 kb ladder
- B; Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit
- Q; Competitor Q kit

Perilla; Genomic DNA from 100 mg leaves of Perilla frutescens var. japonica Regel
B; mean yield; 7.1 μg, mean purity; 1.80 Q; mean yield; 3.5 μg, mean purity; 1.85
Chinese cabbage; Genomic DNA from 100 mg leaves of Brassica rapa var. glabra Regel
B; mean yield; 5.9 μg, mean purity; 1.82 Q; mean yield; 4.7 μg, mean purity; 1.82
Zucchini; Genomic DNA from 100 mg tissue of Cucurbita pepo
B; mean yield; 3.6 μg, mean purity; 1.77 Q; mean yield; 3.0 μg, mean purity; 1.79
Onion; Genomic DNA from 100 mg tissue of Allium cepa
B; mean yield; 5.5 μg, mean purity; 1.80 Q; mean yield; 1.6 μg, mean purity; 1.84
Kidney bean; Genomic DNA from 50 mg seeds of Phaseolus vulgrais
B; mean yield; 7.7 μg, mean purity; 1.73 Q; mean yield; 6.6 μg, mean purity; 1.80

Figure 2. Comparison of genomic DNA from Brassica oleracea var. italic (broccoli) tissues purified with MagListo™ 5M Plant Genomic DNA Extraction Kit and that done with a competitor’s kit (single column type).

M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from broccoli (100 mg) purified with Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit, mean yield; 4.7 μg, mean purity; 1.85
3-4; (Mini)Genomic DNA broccoli (100mg) purified with competitor Q kit, mean yield; 3.7 μg, mean purity; 1.84
5-6; (Midi)Genomic DNA from broccoli (500mg), mean yield; 25.9 μg, mean purity; 1.86
7-8; (Maxi)Genomic DNA from broccoli (1g), mean yield; 48.2 μg, mean purity; 1.84

Figure 3. Comparison of genomic DNA from Cucumis sativus (cucumber) seeds purified with MagListo™ 5M Plant Genomic DNA Extraction Kit and that done with a competitor’s kit (single column type).

M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from cucumber seed (50 mg) purified with Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit, mean yield; 2.8 μg, mean purity; 1.68
3-4; (Mini)Genomic DNA from cucumber seed (50 mg) purified with competitor Q kit, mean yield; 1.9 μg, mean purity; 1.83
5-6; (Midi)Genomic DNA from cucumber seed (250 mg), mean yield; 12.45 μg, mean purity; 1.69
7; (Maxi) Genomic DNA from cucumber seed (500 mg), yield; 20.8 μg, purity; 1.71

Figure 4. Electrophoresis data of conventional PCR of plant genomic DNA purified with MagListo™ 5M Genomic DNA Extraction Kit and those done with a competitor’s kit (single column type).

M; Bioneer 100 bp ladder
1-2; 100 bp fragment of EMBRYONIC FLOWER 2 (EMF2) gene from broccoli genomic DNA purified with Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit
3-4; 100 bp fragment of EMBRYONIC FLOWER 2 (EMF2) gene from broccoli genomic DNA purified with competitor Q kit
5-6; 180 bp fragment of glyceraldehyde-3-phosphate dehydrogenase A (GAPDH) gene from cucumber seed genomic DNA purified with Bioneer MagListo™ 5M Plant Genomic DNA Extraction Kit
7-8; 180 bp fragment of glyceraldehyde-3-phosphate dehydrogenase A (GAPDH) gene from cucumber genomic DNA purified with competitor Q kit

Literature/Support